Direct identification of bifidobacteria from probiotic supplements
Bunešová V., Vlková E., Rada V., Hovorková P., Ročková Š., Kmeť V.:https://doi.org/10.17221/291/2013-CJFSCitation:Bunešová V., Vlková E., Rada V., Hovorková P., Ročková Š., Kmeť V.: (2014): Direct identification of bifidobacteria from probiotic supplements. Czech J. Food Sci., 32: 132-136.
The DNA of 14 probiotic supplements was isolated directly from various products without previous cultivation. The bifidobacteria composition declared by the manufacturer was determined by species-specific PCR. Such species found were in accordance with those listed on the products. This approach can be performed in less than 5 h and is applicable to other genera of probiotic bacteria. Bifidobacteria were also detected by culture-dependent analysis and MALDI-TOF MS. These methods drew the same results; however, they are more expensive and time consuming. So, we propose direct identification of bifidobacteria for routine quality control inspections of species composition in probiotic supplements.Keywords:
Bifidobacterium; species-specific identification