Grapevine extracts and their effect on selected gut-associated microbiota: In vitro study

Rollová M., Gharwalova L., Krmela A., Schulzová V., Hajšlová J., Jaroš P., Kolouchová I., Maťátková O. (2020): Grapevine extracts and their effect on selected gut-associated microbiota: In vitro study. Czech J. Food Sci., 38: 137-143.

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The biological activity of polyphenol substances contained in food supplements prepared from Vitis vinifera can affect the microorganisms present in the digestive tract in terms of their representation and activity of the individual species. This study deals with resveratrol and two polyphenol-rich extracts (extract from V. vinifera canes and the commercial product Regrapex-R-forte) and their effect on selected gut microbiota (Bifidobacterium animalis subsp. lactis Bb-12, Lactobacillus acidophilus LA-5, Lactobacillus casei Lafti L-26, Citrobacter freundii DBM 3127, Escherichia coli DBM 3125). The effect of the studied agents on planktonic and biofilm growth of the microorganisms was determined as minimum inhibitory concentration (MIC80) and minimum biofilm inhibitory concentration (MBIC80), respectively. The extracts induced metabolic activity as well as total biofilm biomass production in probiotic strain L. acidophilus LA-5 while successfully inhibiting the growth of opportunistic pathogenic microorganisms C. freundii DBM 3127 and E. coli DBM 3125.

Andrews J.M. (2001): Determination of minimum inhibitory concentrations. Journal of Antimicrobial Chemotherapy, 48: 5–16.
Arcanjo N.O., Andrade M.J., Padilla P., Rodríguez A., Madruga M.S., Estévez M. (2019): Resveratrol protects Lactobacillus reuteri against H2O2-induced oxidative stress and stimulates antioxidant defenses through upregulation of the dhaT gene. Free Radical Biology and Medicine, 135: 38–45.
Bostanghadiri N., Pormohammad A., Chirani A.S., Pouriran R., Erfanimanesh S., Hashemi A. (2017): Comprehensive review on the antimicrobial potency of the plant polyphenol resveratrol. Biomedicine & Pharmacotherapy, 95: 1588–1595.
Gharwalova L., Hutar D., Masak J., Kolouchova I. (2018): Antioxidant activity and phenolic content of organic and conventional vine cane extracts. Czech Journal of Food Sciences, 36: 289–295.
Goto K., Kanaya S., Ishigami T., Hara Y. (1999): The effects of tea catechins on fecal conditions of elderly residents in a long-term care facility. Journal of Nutritional Science and Vitaminology, 45: 135–141.
Houille B., Besseau S., Courdavault V., Oudin A., Glevarec G., Delanoue G., Guerin L., Simkin A.J., Papon N., Clastre M., Giglioli-Guivarc’h N., Lanoue A. (2015): Biosynthetic origin of e-resveratrol accumulation in grape canes during postharvest storage. Journal of Agricultural and Food Chemistry, 63: 1631–1638.
Chatterjee A., Yasmin T., Bagchi D., Stohs S.J. (2003): The bactericidal effects of Lactobacillus acidophilus, garcinol and Protykin compared to clarithromycin, on Helicobacter pylori. Molecular and Celluar Biochemistry, 243: 29–35.
Kolouchová I., Maťátková O., Paldrychová M., Kodeš Z., Kvasničková E., Sigler K., Čejková A., Šmidrkal J., Demnerová K., Masák J. (2018): Resveratrol, pterostilbene, and baicalein: Plant-derived anti-biofilm agents. Folia Microbiologica, 63: 261–272.
Kolouchová I., Melzoch K., Šmidrkal J., Filip V. (2005): The content of resveratrol in vegetables and fruit. Chemické listy, 99: 492–495. (In Czech)
Larrosa M., Yanez-Gascon M.J., Selma M.V., Gonzalez-Sarrias A., Toti S., Ceron J.J., Tomas-Barberan F., Dolara P., Espin J. C. (2009): Effect of a low dose of dietary resveratrol on colon microbiota, inflammation and tissue damage in a DSS-induced colitis rat model. Journal of Agricultural and Food Chemistry, 57: 2211–2220.
Lavelli V., Sri Harsha P.S., Torri L., Zeppa G. (2014): Use of winemaking by-products as an ingredient for tomato puree: The effect of particle size on product quality. Food Chemitry, 152: 162–168.
Lee H.C., Jenner A.M., Low C.S., Lee Y.K. (2006): Effect of tea phenolics and their aromatic fecal bacterial metabolites on intestinal microbiota. Research in Microbiology, 157: 876–884.
Li Z., Summanen P.H., Komoriya T., Henning S.M., Lee R.P., Carlson E., Heber D., Finegold S.M. (2015): Pomegranate ellagitannins stimulate growth of gut bacteria in vitro: Implications for prebiotic and metabolic effects. Anaerobe, 34: 164–168.
Ma G., Chen Y. (2020): Polyphenol supplementation benefits human health via gut microbiota: A systematic review via meta-analysis. Journal of Functional Foods, 66: 103829.
O’Connor D.J., Wong R.W., Rabie A.B. (2011): Resveratrol inhibits periodontal pathogens in vitro. Phytotherapy Research, 25: 1727–1731.
Paldrychová M., Kolouchová I., Vaňková E., Maťátková O., Šmidrkal J., Krmela A., Schulzová V., Hajšlová J., Masák J. (2019): Effect of resveratrol and Regrapex-R-forte on Trichosporon cutaneum biofilm. Folia Microbiologica 64: 73–81.
Peeters E., Nelis H.J., Coenye T. (2008): Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. Journal of Microbiological Methods, 72: 157–165.
Ravindran R., Jaiswal A.K. (2016): Exploitation of food industry waste for high-value products. Trends in Biotechnology, 34: 58–69.
Riss T.L., Moravec R.A., Niles A.L., Duellman S., Benink H.A., Worzella T.J., Minor L. (2013): Cell Viability Assays. In: Sittampalam G.S., Coussens N.P., Brimacombe K. et al. (eds): Assay Guidance Manual. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences: 1–6
Sabaeifard P., Abdi-Ali A., Soudi M.R., Dinarvand R. (2014): Optimization of tetrazolium salt assay for Pseudomonas aeruginosa biofilm using microtiter plate method. Journal of Microbiological Methods, 105: 134–140.
Sankar S.A., Lagier J.C., Pontarotti P., Raoult D., Fournier P.E. (2015): The human gut microbiome, a taxonomic conundrum. Systematic and Applied Microbiology, 38: 276–286.
Taguri T., Tanaka T., Kouno I. (2006): Antibacterial spectrum of plant polyphenols and extracts depending upon hydroxyphenyl structure. Biological and Pharmaceutical Bulletin, 29: 2226–2235.
Yamakoshi Y., Tokutake S., Kikuchi M., Kubota Y., Konishi H., Mitsuoka T. (2001): Effect of proanthocyanidin-rich extract from grape seeds on human fecal flora and fecal odor. Microbial Ecology in Health and Disease, 13: 25–31.
Zhou L., Wang W., Huang J., Ding Y., Pan Z., Zhao Y., Zhang R., Hu B., Zeng X. (2016): In vitro extraction and fermentation of polyphenols from grape seeds (Vitis vinifera) by human intestinal microbiota. Food & Function, 7: 1959–1967.
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