Quality control of chondroitin sulphate used in dietary supplements

https://doi.org/10.17221/326/2014-CJFSCitation:Václavíková E., Kvasnička F. (2015): Quality control of chondroitin sulphate used in dietary supplements. Czech J. Food Sci., 33: 165-173.
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Chondroitin sulphate (CS), a complex polysaccharide isolated from animal cartilage, is used as an ingredient in dietary supplements to support joint health and/or to treat osteoarthritis. Several low-quality CS raw materials were identified in marketed products of dietary supplements. For the evaluation of the quality of CS, we present here a capillary isotachophoretic method (cITP). As a leading electrolyte for the anionic analysis of CS, a mixture of 5 mM HCl + 10 mM glycine + 0.01% of 2-hydroxyethylcellulose of pH 2.8 was used. A solution of 10 mM citric acid served as the terminating electrolyte. On a series of CS raw material samples we proved that the cITP is a suitable method for this purpose.
References:
Choi Don Woong, Kim Mi Jung, Kim Hee Sung, Chang Soo Hyun, Jung Gi Sook, Shin Kyung Yong, Chang Seung Yeup (2003): A size-exclusion HPLC method for the determination of sodium chondroitin sulfate in pharmaceutical preparations. Journal of Pharmaceutical and Biomedical Analysis, 31, 1229-1236  https://doi.org/10.1016/S0731-7085(02)00731-8
 
Grøndahl Frøy, Tveit Heidi, Akslen-Hoel Linn Kristin, Prydz Kristian (2011): Easy HPLC-based separation and quantitation of chondroitin sulphate and hyaluronan disaccharides after chondroitinase ABC treatment. Carbohydrate Research, 346, 50-57  https://doi.org/10.1016/j.carres.2010.10.025
 
Grossman P.D., Colburn J.C. (eds) (1992): Capillary Electrophoresis: Theory and Practice. San Diego, Academic Press, Inc.
 
Grossman Paul D., Soane David S. (1991): Experimental and theoretical studies of DNA separations by capillary electrophoresis in entangled polymer solutions. Biopolymers, 31, 1221-1228  https://doi.org/10.1002/bip.360311010
 
Jin Pengfei, Ma Jie, Wu Xuejun, Zou Ding, Sun Chunhua, Hu Xin (2009): Simultaneous determination of chondroitin sulfate sodium, allantoin and pyridoxine hydrochloride in pharmaceutical eye drops by an ion-pair high-performance liquid chromatography. Journal of Pharmaceutical and Biomedical Analysis, 50, 293-297  https://doi.org/10.1016/j.jpba.2009.04.037
 
Malavaki Christina J., Asimakopoulou Athanasia P., Lamari Fotini N., Theocharis Achilleas D., Tzanakakis George N., Karamanos Nikos K. (2008): Capillary electrophoresis for the quality control of chondroitin sulfates in raw materials and formulations. Analytical Biochemistry, 374, 213-220  https://doi.org/10.1016/j.ab.2007.11.006
 
Okamoto Hitoshi, Nakajima Toshiaki, Ito Yuji, Shimada Kenji, Yamato Susumu (2004): Development of a novel analytical method for determination of chondroitin sulfate using an in-capillary enzyme reaction. Journal of Chromatography A, 1035, 137-144  https://doi.org/10.1016/j.chroma.2004.02.051
 
Sim Joon-Soo, Jun Gyungjin, Toida Toshihiko, Cho So Yean, Choi Don Woong, Chang Seung-Yeup, Linhardt Robert J., Kim Yeong Shik (2005): Quantitative analysis of chondroitin sulfate in raw materials, ophthalmic solutions, soft capsules and liquid preparations. Journal of Chromatography B, 818, 133-139  https://doi.org/10.1016/j.jchromb.2004.12.015
 
Tømmeraas Kristoffer, Wahlund Per-Olof (2009): Poly-acid properties of biosynthetic hyaluronan studied by titration. Carbohydrate Polymers, 77, 194-200  https://doi.org/10.1016/j.carbpol.2008.12.021
 
Václavíková E., Kvasnička F. (2013): Isotachophoretic determination of glucosamine and chondroitin sulphate in dietary supplements. Czech Journal of Food Sciences, 31: 55–65.
 
Volpi Nicola (2007): Analytical aspects of pharmaceutical grade chondroitin sulfates. Journal of Pharmaceutical Sciences, 96, 3168-3180  https://doi.org/10.1002/jps.20997
 
Volpi Nicola (2009): Quality of different chondroitin sulfate preparations in relation to their therapeutic activity. Journal of Pharmacy and Pharmacology, 61, 1271-1280  https://doi.org/10.1211/jpp.61.10.0002
 
Volpi Nicola, Maccari Francesca (2008): Quantitative and Qualitative Evaluation of Chondroitin Sulfate in Dietary Supplements. Food Analytical Methods, 1, 195-204  https://doi.org/10.1007/s12161-008-9020-9
 
Wang H., Loganathan D., Lindhardt R.J. (1991): Determination of the pKa of glucuronic acid and the carboxy groups of heparin by 13C-nuclear-magnetic-resonance spectroscopy. Biochemistry Journal, 278: 689–695.
 
Yamamoto S., Ohta D., Morikawa Y., Bunseki Kagaku (1982): Determination of sodium chondroitin sulfate in cosmetic products by capillary tube isotachophoresis. Bunseki Kagaku (Jap. Anal.), 31: 251–254.
 
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