Determining the optimal method for DNA isolation from fruit jamsá T., Křížová B., Ovesná J. (2018): Determining the optimal method for DNA isolation from fruit jams. Czech J. Food Sci., 36: 126-132.
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DNA extraction is a crucial step in PCR analysis especially when analysing food samples that can be degraded and can potentially contain PCR-inhibiting substances. In this study, we compared the suitability of three DNA extraction methods – two kits: DNeasy® Plant Mini Kit and NucleoSpin® Food, and the CTAB method – for DNA extraction from commercial fruit jams. Fourteen jams with different contents of fruit, sugar and other additives were extracted in triplicate using the above-mentioned methods directly and after a washing step. The concentration and optical density were analysed using UV spectrophotometry and the amplifiability of the obtained DNA was evaluated using a PCR assay targeting a sequence coding for chloroplast tRNA-Leu. Samples isolated using the NucleoSpin® Food kit contained non-amplifiable DNA in eight cases, and samples isolated using the CTAB method could not be quantified. The DNeasy® Plant Mini Kit thus proved to be the most suitable method, since well-amplifiable DNA was obtained for all the analysed samples.

Allen G C, Flores-Vergara M A, Krasynanski S, Kumar S, Thompson W F (2006): A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide. Nature Protocols, 1, 2320-2325
Anvoh K.Y.B., Bi A. Zoro, Gnakri D. (2009): Production and Characterization of Juice from Mucilage of Cocoa Beans and its Transformation into Marmalade. Pakistan Journal of Nutrition, 8, 129-133
Arleo M., Ruibal F., Pereyra J., Miquel E., Fernández M., Martínez C. (2012): A DNA-based approach to discriminate between quince and apple in quince jams. International Food Research Journal, 19: 1471–1477.
Clarke M.-A.L., Dooley J.J., Garrett S.D., Brown H.M. (2008): An investigation into the use of PCR–RFLP profiling for the identification of fruit species in fruit juices. Food Standards Agency. Project code: Q01111.
Chapela María José, Sotelo Carmen G., Pérez-Martín Ricardo I., Pardo Miguel Ángel, Pérez-Villareal Begoña, Gilardi Patricia, Riese Juan (2007): Comparison of DNA extraction methods from muscle of canned tuna for species identification. Food Control, 18, 1211-1215
Deer D.M., Lampel K.A., González-Escalona N. (2010): A versatile internal control for use as DNA in real-time PCR and as RNA in real-time reverse transcription PCR assays. Letters in Applied Microbiology, 50, 366-372
Defernez Marianne, Wilson Reginald H (1995): Mid-infrared spectroscopy and chemometrics for determining the type of fruit used in jam. Journal of the Science of Food and Agriculture, 67, 461-467
Bernardo G. Di, Galderisi U., Cipollaro M., Cascino A. (2005): Methods To Improve the Yield and Quality of DNA from Dried and Processed Figs. Biotechnology Progress, 21, 546-549
Pinto Angela Di, Forte VitoTony, Guastadisegni Maria Corsignano, Martino Carmela, Schena Francesco Paolo, Tantillo Giuseppina (2007): A comparison of DNA extraction methods for food analysis. Food Control, 18, 76-80
Egbekun M.K., Nda-Suleiman E.O., Akinyeye O. (1998): Utilization of fluted pumpkin fruit (Telfairia occidentalis) in marmalade manufacturing. Plant Foods for Human Nutrition, 52: 171–176.
Fügel R., Carle R., Schieber A. (2005): Quality and authenticity control of fruit purées, fruit preparations and jams – a review. Trends in Food Science & Technology, 16: 433–441.
Ganopoulos Ioannis, Argiriou Anagnostis, Tsaftaris Athanasios (2011): Microsatellite high resolution melting (SSR-HRM) analysis for authenticity testing of protected designation of origin (PDO) sweet cherry products. Food Control, 22, 532-541
Hedman J., Rådström P. (2013): Overcoming Inhibition in Real-Time Diagnostic PCR. In: Wilks M. (ed.): PCR Detection of Microbial Pathogens. New York, Humana Press: 17–48.
Holden Marcia J., Haynes Ross J., Rabb Savelas A., Satija Neena, Yang Kristina, Blasic Joseph R. (2009): Factors Affecting Quantification of Total DNA by UV Spectroscopy and PicoGreen Fluorescence. Journal of Agricultural and Food Chemistry, 57, 7221-7226
Jaakola Laura, Suokas Marko, Häggman Hely (2010): Novel approaches based on DNA barcoding and high-resolution melting of amplicons for authenticity analyses of berry species. Food Chemistry, 123, 494-500
Mafra Isabel, Ferreira Isabel M. P. L. V. O., Oliveira M. Beatriz P. P. (2008): Food authentication by PCR-based methods. European Food Research and Technology, 227, 649-665
Mekuria Genet T., Collins Graham G., Sedgley Margaret (2015): Genetic variability between different accessions of some common commercial olive cultivars. The Journal of Horticultural Science and Biotechnology, 74, 309-314
Meyer Rolf (1999): Development and application of DNA analytical methods for the detection of GMOs in food. Food Control, 10, 391-399
Murray M.G., Thompson W.F. (1980): Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8, 4321-4326
Navarro-Pascual-Ahuir María, Lerma-García María Jesús, Simó-Alfonso Ernesto F., Herrero-Martínez José Manuel (2015): Rapid Differentiation of Commercial Juices and Blends by Using Sugar Profiles Obtained by Capillary Zone Electrophoresis with Indirect UV Detection. Journal of Agricultural and Food Chemistry, 63, 2639-2646
Olexov� L�dia, Kuchta Tom�?, Dovicovicov� Lubomira (2004): Comparison of three types of methods for the isolation of DNA from flours, biscuits and instant paps. European Food Research and Technology, 218, 390-393
Pandey Ram Naresh, Adams Robert P., Flournoy Lori E. (1996): Inhibition of random amplified polymorphic DNAs (RAPDs) by plant polysaccharides. Plant Molecular Biology Reporter, 14, 17-22
Peist R., Honsel D., Twieling G., Löffert D. (2001): PCR inhibitors in plant DNA preparations. Qiagen news, 3: 7–9.
Qiu S. Q., Liu Kede, Jiang J. X., Song X., Xu C. G., Li X. H., Zhang Qifa (2005): Delimitation of the rice wide compatibility gene S5 n to a 40-kb DNA fragment. Theoretical and Applied Genetics, 111, 1080-1086
Renault T., Arzul I., Lipart C. (2004): Development and use of an internal standard for oyster herpesvirus 1 detection by PCR. Journal of Virological Methods, 121, 17-23
Siret R., Boursiquot J. M., Merle M. H., Cabanis J. C., This P. (2000): Toward the Authentication of Varietal Wines by the Analysis of Grape ( Vitis vinifera L.) Residual DNA in Must and Wine Using Microsatellite Markers. Journal of Agricultural and Food Chemistry, 48, 5035-5040
Taberlet Pierre, Gielly Ludovic, Pautou Guy, Bouvet Jean (1991): Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology, 17, 1105-1109
Turci Manuela, Sardaro Maria Luisa Savo, Visioli Giovanna, Maestri Elena, Marmiroli Marta, Marmiroli Nelson (2010): Evaluation of DNA extraction procedures for traceability of various tomato products. Food Control, 21, 143-149
Varma Astha, Padh Harish, Shrivastava Neeta (2007): Plant genomic DNA isolation: An art or a science. Biotechnology Journal, 2, 386-392
Voragen Alphons G. J., Coenen Gerd-Jan, Verhoef René P., Schols Henk A. (2009): Pectin, a versatile polysaccharide present in plant cell walls. Structural Chemistry, 20, 263-275
Yamamoto Toshiya, Kimura Tetsuya, Hayashi Tateki, Ban Yoshiyuki (2006): DNA Profiling of Fresh and Processed Fruits in Pear. Breeding Science, 56, 165-171
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