Honey sold directly by producers in the Silesian region of Poland as a source of Clostridium botulinum
types A, B, E and F

https://doi.org/10.17221/376/2016-CJFSCitation:Hayıt F., Gül H. (2017): Honey sold directly by producers in the Silesian region of Poland as a source of Clostridium botulinum types A, B, E and F. Czech J. Food Sci., 35: 194-199.
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The level of contamination of honey with Clostridium botulinum spores is considered as an indicator of the adequacy of hygienic practices during collection, extraction, and subsequent processing. A total of 39 honey samples purchased directly from beekeepers at outdoor markets and from small amateur apiaries in Silesia were analysed for Clostridium botulinum spores. The samples were prepared using a dilution centrifugation method and cultured in parallel in cooked meat medium (CMM) and tripticase peptone glucose yeast (TPGY) enrichment broths. Identification of C. botulinum toxin types A, B, E, and F was performed with the use of a multiplex PCR method. The analysis showed six (15.4%) samples to be contaminated with C. botulinum spores. The major serotypes detected were type A – in two (5.1%) and type B – in two (5.1%) honey samples, respectively. Types E and F were found in 1 (2.6%) and 1 (2.6%) positive honey sample analysed, respectively.
References:
Arnon S.S. (1998): Infant botulism. In: Feifen R.D., Cherry J.D., Saunders W.B. (eds): Textbook of Pediatric Infectious Diseases. Philadelphia, Elsevier Inc.: 1758–1766.
 
Arnon Stephen S., Midura Thaddeus F., Damus Karla, Thompson Barbara, Wood Ronald M., Chin James (1979): Honey and other environmental risk factors for infant botulism. The Journal of Pediatrics, 94, 331-336 https://doi.org/10.1016/S0022-3476(79)80863-X
 
Bianco M.I., Luquez C., de Jong L.I.T., Fernanadez R.A. (2009): Linden flower (Tilia spp) as potential vehicle of Clostridium botulinum spores in the transmission of infant botulism. Revista Argentina de Microbiología, 41: 232–236.
 
Böhnel Helge, Behrens Susanne, Loch Petra, Lube Karin, Gessler Frank (2001): Is there a link between infant botulism and sudden infant death? Bacteriological results obtained in Central Germany. European Journal of Pediatrics, 160, 623-628 https://doi.org/10.1007/s004310100807
 
Estevinho Leticia M., Feás Xesús, Seijas Julio A., Pilar Vázquez-Tato M. (2012): Organic honey from Trás-Os-Montes region (Portugal): Chemical, palynological, microbiological and bioactive compounds characterization. Food and Chemical Toxicology, 50, 258-264 https://doi.org/10.1016/j.fct.2011.10.034
 
Fenicia L., Anniballi F., De Medici D., Delibato E., Aureli P. (): SYBR Green Real-Time PCR Method To Detect Clostridium botulinum Type A. Applied and Environmental Microbiology, 73, 2891-2896 https://doi.org/10.1128/AEM.02234-06
 
Gale P., Drew T., Phipps L.P., David G., Wooldridge M. (2009): The effect of climate change on the occurrence and prevalence of livestock diseases in Great Britain: a review. Journal of Applied Microbiology, 106, 1409-1423 https://doi.org/10.1111/j.1365-2672.2008.04036.x
 
Huss H.H. (1980): Distribution of Clostridium botulinum. Applied and Environmental Microbiology, 39: 764–769.
 
Kędzia B., Kędzia A., Hołderna-Kędzia E. (1996): Badania nad występowaniem w miodzie przetrwalników laseczek Bacillus i Clostridium. In: Proceedings XXXIIIth Naukowa Konferencja Pszczelarska, March 12–13, 1996, Puławy, Poland: 37–39.
 
Kędzia B., Hołderna-Kędzia E. (2010): Lecznicze działanie miodu pszczelego w chorobach wewnętrznych. Wrocław, MedPharm Polska: 363–372.
 
KOLUMAN Ahmet, MELİKOĞLU GÖLCÜ Berfin, DERİN Okan, ÖZKÖK Sibel, ANNIBALLI Fabrizio (2013): Clostridium botulinum in honey: prevalence and antibiotic susceptibility of isolated strains. TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES, 37, 706-711 https://doi.org/10.3906/vet-1209-40
 
Küplülü Özlem, Göncüoğlu Muammer, Özdemir Haydar, Koluman Ahmet (2006): Incidence of Clostridium botulinum spores in honey in Turkey. Food Control, 17, 222-224 https://doi.org/10.1016/j.foodcont.2004.10.004
 
Loncaric Igor, Ruppitsch Werner, Licek Elisabeth, Moosbeckhofer Rudolf, Busse Hans-Jürgen, Rosengarten Renate (2011): Characterization of selected Gram-negative non-fermenting bacteria isolated from honey bees (Apis mellifera carnica). Apidologie, 42, 312-325 https://doi.org/10.1007/s13592-011-0019-7
 
Luquez C., Bianco M. I., de Jong L. I. T., Sagua M. D., Arenas G. N., Ciccarelli A. S., Fernandez R. A. (): Distribution of Botulinum Toxin-Producing Clostridia in Soils of Argentina. Applied and Environmental Microbiology, 71, 4137-4139 https://doi.org/10.1128/AEM.71.7.4137-4139.2005
 
Madras-Majewska B., Nowakowska A., Rosiak E. (2014): Evaluation of microbial quality of imported honeys available on the Warsaw market. In: Proceedings of International Symposium on Bee Products. 3rd Ed. Annual meeting of the International Honey Commission (IHC), Sept 28–Oct 1, 2014, Opatija, Croatia: 117.
 
Nakano Hiroyuki, Kizaki Hideki, Sakaguchi Genji (1994): Multiplication of Clostridium botulinum in dead honey-bees and bee pupae, a likely source of heavy contamination of honey. International Journal of Food Microbiology, 21, 247-252 https://doi.org/10.1016/0168-1605(94)90031-0
 
Nevas Mari, Hielm Sebastian, Lindström Miia, Horn Helmut, Koivulehto Kari, Korkeala Hannu (2002): High prevalence of Clostridium botulinum types A and B in honey samples detected by polymerase chain reaction. International Journal of Food Microbiology, 72, 45-52 https://doi.org/10.1016/S0168-1605(01)00615-8
 
NEVAS M, LINDSTROM M, HAUTAMAKI K, PUOSKARI S, KORKEALA H (2005): Prevalence and diversity of Clostridium botulinum types A, B, E and F in honey produced in the Nordic countries. International Journal of Food Microbiology, 105, 145-151 https://doi.org/10.1016/j.ijfoodmicro.2005.04.007
 
Nevas Mari, Lindstrom Miia, Horman Ari, Keto-Timonen Riikka, Korkeala Hannu (2006): Contamination routes of Clostridium botulinum in the honey production environment. Environmental Microbiology, 8, 1085-1094 https://doi.org/10.1111/j.1462-2920.2006.001000.x
 
Palacio M. Alejandra, Figini Emilio E., Ruffinengo Sergio R., Rodriguez Edgardo M., del Hoyo Marcelo L., Bedascarrasbure Enrique L. (2000): Changes in a population of Apis mellifera L.selected for hygienic behaviourand its relation to brood disease tolerance. Apidologie, 31, 471-478 https://doi.org/10.1051/apido:2000139
 
PN-88/A-77626 (1988): “Miód pszczeli”. Dziennik Norm i Miar nr 8. Warszawa, Polska, Wydawnictwo Normalizacyjne Alfa.
 
Roman Adam, Popiela-Pleban Ewa, Kozak Maria (2013): Factors influencing consumer behavior relating to the purchasing of honey part 1. The buying process and the level of consumption. Journal of Apicultural Science, 57, - https://doi.org/10.2478/jas-2013-0026
 
Różańska H. (2011): Microbiological quality of Polish honey. Bulletin of the Veterinary Institute in Pulawy, 55: 443–445.
 
Satorres Sara E., Alcaráz Lucı́a E., Fernandez Rafael, Puig de Centorbi Olga N. (1999): Isolation of Clostridium botulinum in Medicinal Plants. Anaerobe, 5, 173-175 https://doi.org/10.1006/anae.1999.0205
 
Semkiw P., Ochal J. (2012): Sektor pszczelarski w Polsce w 2012 roku. Instytut Ogrodnictwa Oddział Pszczelnictwa, Puławy. Available at: www.opisik.pulawy.pl/pdf/SP2012.pdf (accessed August 22, 2016)
 
Sobel J. (2005): Botulism. Clinical Infectious Diseases, 41, 1167-1173 https://doi.org/10.1086/444507
 
Spivak M., Downey D. L. (1998): Field Assays for Hygienic Behavior in Honey Bees (Hymenoptera: Apidae). Journal of Economic Entomology, 91, 64-70 https://doi.org/10.1093/jee/91.1.64
 
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