Study on the ageing method and antioxidant activity of black garlic residues
Feng Xiong, Chunhua Dai, Furong Hou, Peipei Zhu, Ronghai He, Haile Mahttps://doi.org/10.17221/420/2016-CJFSCitation:Xiong F., Dai C., Hou F., Zhu P., He R., Ma H. (2018): Study on the ageing method and antioxidant activity of black garlic residues. Czech J. Food Sci., 36: 88-97.
Garlic residue (GR), a co-product of garlic oil extraction, contains most of the nutrients of raw garlic (RG). Preparation of black garlic residue (BGR) is considered to be an effective method of processing GR. The main objective of this study was to optimise the ageing conditions of GR based on moisture, polyphenol and 5-hydroxymethyl-2-furaldehyde (HMF) levels. In addition, the antioxidant capacity of BGR was also evaluated in vitro and in vivo and compared with black garlic (BR) and RG. The results indicate that optimum ageing resulting in polyphenol and HMF contents of 25.80 mg/g and 3.84 mg/g, respectively, were achieved using a temperature of 90°C and humidity of 95% for four days. Both BGR and BR had stronger capacities to scavenge α,α-diphenyl-β-picrylhydrazyl (DPPH) than RG with half maximal inhibitory concentration (IC50) values of 0.454, 0.514 and 4.236 mg/ml, respectively. Experiments on mice demonstrated that there was no obvious difference in antioxidant activity between BGR and BR in vivo. BGR and BR consumption significantly decreased malondialdehyde (MDA) levels in serum and liver, in addition to markedly increasing superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities.Keywords:
ageing conditions; antioxidant ability; garlic residues productReferences:
Toledano-Medina M. Angeles, Pérez-Aparicio Jesús, Moreno-Rojas Rafael, Merinas-Amo Tania (2016): Evolution of some physicochemical and antioxidant properties of black garlic whole bulbs and peeled cloves. Food Chemistry, 199, 135-139 https://doi.org/10.1016/j.foodchem.2015.11.128Bae Sang Eun, Cho Seung Yong, Won Yong Duk, Lee Seon Ha, Park Hyun Jin (2012): A comparative study of the different analytical methods for analysis of S-allyl cysteine in black garlic by HPLC. LWT - Food Science and Technology, 46, 532-535 https://doi.org/10.1016/j.lwt.2011.11.013Durling Louise J.K., Busk Leif, Hellman Björn E. (2009): Evaluation of the DNA damaging effect of the heat-induced food toxicant 5-hydroxymethylfurfural (HMF) in various cell lines with different activities of sulfotransferases. Food and Chemical Toxicology, 47, 880-884 https://doi.org/10.1016/j.fct.2009.01.022Halliwell B., Gutteridge J.M.C. (1990): Role of free radicals and catalytic metal irons in human disease: an overview, Method Enzymol. 186: 59–85.Han D.Q., Zhang J.J. (2008): Application of DNS method to the determination of saccharide content in Pullulan fermentation broth. Science and Technology of Food Industry, 29: 285–290.Huang L., Zhuo S.Z. (2008): Extraction of garlic oil and application of its by-product. Science and Technology of Food Industry, 29: 197–198, 232.Kim S.H., Jung E.Y., Kang D.H., Chang U.J., Hong Y.H., Suh H.J. (2012): Physical stability, antioxidative properties, and photoprotective effects of a functionalized formulation containing black garlic extract. Journal of Photochemistry and Photobiology. B Biology, 117: 104–110.Kim J.S., Kang O.J., Gweon O.C. (2013a): Changes in the content of fat-and water-soluble vitamins in black garlic at the different thermal processing steps. Food Science and Biotechnology, 22: 283–287.Kim J.S., Kang O.J., Gweon O.C. (2013b): Comparison of phenolic acids and flavonoids in black garlic at different thermal processing steps. Journal of Functional Foods, 5: 80–86.Leong Jasmine, Morel Patrick C.H., Purchas Roger W., Wilkinson Brian H.P. (2010): The production of pork with garlic flavour notes using garlic essential oil. Meat Science, 84, 699-705 https://doi.org/10.1016/j.meatsci.2009.11.006Li W.X., Yu D., Lin L., Sun J., Lan Z.W., Huo J.S. (2011): Study on the determination of total polyphenols content in vegetables and fruits by folin-ciocalteu colorimetry. Acta Nutrimenta Sinica, 3: 302–307.Li Ningyang, Lu Xiaoming, Pei Houbao, Qiao Xuguang (2015): Effect of Freezing Pretreatment on the Processing Time and Quality of Black Garlic. Journal of Food Process Engineering, 38, 329-335 https://doi.org/10.1111/jfpe.12156Liao W.Z., Ning Z.X., Chen L.X., Wei Q.Y., Yuan E.D., Yang J.G., Ren J. (2014): Intracellular antioxidant detoxifying effects of diosmetin on 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress through inhibition of reactive oxygen species generation. Journal of Agricultural & Food Chemistry, 62: 8648–8654.MacDonald-Wicks Lesley K, Wood Lisa G, Garg Manohar L (2006): Methodology for the determination of biological antioxidant capacityin vitro: a review. Journal of the Science of Food and Agriculture, 86, 2046-2056 https://doi.org/10.1002/jsfa.2603Moustafa G.G., Hussein M.M.A. (2016). New insight on using aged garlic extract against toxic impacts of titanium dioxide bulk salt triggers inflammatory and fibrotic cascades in male rats. Biomedicine & Pharmacotherapy, 84: 687–697.Naderi R, Mohaddes G, Mohammadi M, Alihemmati A, Badalzadeh R, Ghaznavi R, Ghyasi R, Mohammadi Sh (2015): Preventive effects of garlic ( Allium sativum ) on oxidative stress and histopathology of cardiac tissue in streptozotocin-induced diabetic rats. Acta Physiologica Hungarica, 102, 380-390 https://doi.org/10.1556/036.102.2015.4.5Nencini Cristina, Menchiari Andrea, Franchi Gian Gabriele, Micheli Lucia (2011): In vitro Antioxidant Activity of Aged Extracts of some Italian Allium Species. Plant Foods for Human Nutrition, 66, 11-16 https://doi.org/10.1007/s11130-010-0204-2Pokluda R., Petrikova K. (2001): Using HPLC for rapid determination of vitamin C in garlic. Horticultural Science, 28: 112–115.Quéguineur Benoît, Goya Luis, Ramos Sonia, Martín Maria Angeles, Mateos Raquel, Bravo Laura (2012): Phloroglucinol: Antioxidant properties and effects on cellular oxidative markers in human HepG2 cell line. Food and Chemical Toxicology, 50, 2886-2893 https://doi.org/10.1016/j.fct.2012.05.026Rajani Kanth V., Uma Maheswara Reddy P., Raju T. N. (2008): Attenuation of streptozotocin-induced oxidative stress in hepatic and intestinal tissues of wistar rat by methanolic-garlic extract. Acta Diabetologica, 45, 243-251 https://doi.org/10.1007/s00592-008-0051-xRamalingam M., Kim S.-J. (2013): Insulin on hydrogen peroxide-induced oxidative stress involves ROS/Ca 2+ and Akt/Bcl-2 signaling pathways. Free Radical Research, 48, 347-356 https://doi.org/10.3109/10715762.2013.869588Robards Kevin, Prenzler Paul D, Tucker Greg, Swatsitang Prasan, Glover William (1999): Phenolic compounds and their role in oxidative processes in fruits. Food Chemistry, 66, 401-436 https://doi.org/10.1016/S0308-8146(99)00093-XSallam Kh.I., Ishioroshi M., Samejima K. (2004): Antioxidant and antimicrobial effects of garlic in chicken sausage. LWT - Food Science and Technology, 37, 849-855 https://doi.org/10.1016/j.lwt.2004.04.001Sayin V. I., Ibrahim M. X., Larsson E., Nilsson J. A., Lindahl P., Bergo M. O. (2014): Antioxidants Accelerate Lung Cancer Progression in Mice. Science Translational Medicine, 6, 221ra15-221ra15 https://doi.org/10.1126/scitranslmed.3007653Wang D.Y., Feng J., Liu, J., Yan M. (2010). Black garlic (Allium sativum) extracts enhance the immune system. Medicinal and Aromatic Plant Science and Biotechnology, 4: 37–40.Yang Xingbin, Yang Su, Guo Yurong, Jiao Yadong, Zhao Yan (2013): Compositional characterisation of soluble apple polysaccharides, and their antioxidant and hepatoprotective effects on acute CCl4-caused liver damage in mice. Food Chemistry, 138, 1256-1264 https://doi.org/10.1016/j.foodchem.2012.10.030Zhang Rui Fen, Zhang Fang Xuan, Zhang Ming Wei, Wei Zhen Cheng, Yang Chun Ying, Zhang Yan, Tang Xiao Jun, Deng Yuan Yuan, Chi Jian Wei (2011): Phenolic Composition and Antioxidant Activity in Seed Coats of 60 Chinese Black Soybean (Glycine max L. Merr.) Varieties. Journal of Agricultural and Food Chemistry, 59, 5935-5944 https://doi.org/10.1021/jf201593nZhang K.C., Ji Z.R., He G.L., Zheng J.F., Han W. (2014): Study on extraction of polysaccharides from garlic residue and the hypolipidemic and hypoglycemic function of the garlic residue. Journal of Anhui Agricultural Science and Technology University, 2014-22.Zhang Zesheng, Lei Mengmeng, Liu Rui, Gao Yunfeng, Xu Mengying, Zhang Min (2015): Evaluation of Alliin, Saccharide Contents and Antioxidant Activities of Black Garlic during Thermal Processing. Journal of Food Biochemistry, 39, 39-47 https://doi.org/10.1111/jfbc.12102Zhang Xinyan, Li Ningyang, Lu Xiaoming, Liu Pengli, Qiao Xuguang (2016): Effects of temperature on the quality of black garlic. Journal of the Science of Food and Agriculture, 96, 2366-2372 https://doi.org/10.1002/jsfa.7351Zong W., Zhang W.W., Zhao G.Y., Zhang L.H. (2014): Optimization of process conditions for polysaccharides from garlic residues by ultra-high pressure method. China Condiment, 39: 20–22.