Antioxidant activities of peptide fractions derived from freshwater mussel protein using ultrasound-assisted enzymatic hydrolysis Z., Tian G., Xu Z., Li M., Xu M., Zhou Y., Ren H. (2017): Antioxidant activities of peptide fractions derived from freshwater mussel protein using ultrasound-assisted enzymatic hydrolysis. Czech J. Food Sci., 35: 328-338.
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The freshwater mussel protein was hydrolysed using ultrasound-assisted enzymolysis. Ultrasound-assisted freshwater mussel protein hydrolysates (UPH) were divided into four fractions (> 10, 6–10, 3–6, and < 3 kDa) using ultrafiltration, and the fraction with the highest antioxidant activity was further subdivided into four fractions (F1–F4) using gel chromatography. The amino acid compositions and antioxidant activities (DPPH, hydroxyl and superoxide radical scavenging activities, reducing power, ferrous ion chelating activity, and inhibition of linoleic acid oxidation) of peptide fractions were investigated. The results showed that the antioxidant activity of the < 3 kDa fraction was significantly higher than that of UPH, > 10, 6–10, and 3–6 kDa fractions. The antioxidant activity of F2 was again higher compared with the < 3 kDa fraction and higher than that of F1, F3, and F4. Amino acid analysis showed that the antioxidant activities (except for chelating activity) of peptides increased with increasing hydrophobic amino acid content. The < 3 kDa and F2 fractions exhibited strong inhibition of linoleic acid oxidation, their effects being even better than that of ascorbic acid (Vc) and l-glutathione (GSH). Therefore, these peptide fractions from freshwater mussel may be a potential natural antioxidant that could be added to various foods.
Bougatef Ali, Nedjar-Arroume Naima, Manni Laïla, Ravallec Rozenn, Barkia Ahmed, Guillochon Didier, Nasri Moncef (2010): Purification and identification of novel antioxidant peptides from enzymatic hydrolysates of sardinelle (Sardinella aurita) by-products proteins. Food Chemistry, 118, 559-565
Bougatef Ali, Nedjar-Arroume Naima, Ravallec-Plé Rozenn, Leroy Yves, Guillochon Didier, Barkia Ahmed, Nasri Moncef (2008): Angiotensin I-converting enzyme (ACE) inhibitory activities of sardinelle (Sardinella aurita) by-products protein hydrolysates obtained by treatment with microbial and visceral fish serine proteases. Food Chemistry, 111, 350-356
Chandrasekara Anoma, Shahidi Fereidoon (2011): Inhibitory Activities of Soluble and Bound Millet Seed Phenolics on Free Radicals and Reactive Oxygen Species. Journal of Agricultural and Food Chemistry, 59, 428-436
Chalamaiah M., Dinesh kumar B., Hemalatha R., Jyothirmayi T. (2012): Fish protein hydrolysates: Proximate composition, amino acid composition, antioxidant activities and applications: A review. Food Chemistry, 135, 3020-3038
China Food Nutrition Network (2010): Food composition database. Available at (accessed June 19, 2017).
Epstein Franklin H., Diaz Marco N., Frei Balz, Vita Joseph A., Keaney John F. (1997): Antioxidants and Atherosclerotic Heart Disease. New England Journal of Medicine, 337, 408-416
Duh Pin-Der (1998): Antioxidant activity of burdock (Arctium lappa Linné): Its scavenging effect on free-radical and active oxygen. Journal of the American Oil Chemists' Society, 75, 455-461
Sabeena Farvin K.H., Andersen Lisa Lystbæk, Nielsen Henrik Hauch, Jacobsen Charlotte, Jakobsen Greta, Johansson Inez, Jessen Flemming (2014): Antioxidant activity of Cod (Gadus morhua) protein hydrolysates: In vitro assays and evaluation in 5% fish oil-in-water emulsion. Food Chemistry, 149, 326-334
Girgih Abraham T., Udenigwe Chibuike C., Hasan Fida M., Gill Tom A., Aluko Rotimi E. (2013): Antioxidant properties of Salmon (Salmo salar) protein hydrolysate and peptide fractions isolated by reverse-phase HPLC. Food Research International, 52, 315-322
Girgih Abraham T., He Rong, Hasan Fida M., Udenigwe Chibuike C., Gill Tom A., Aluko Rotimi E. (2015): Evaluation of the in vitro antioxidant properties of a cod (Gadus morhua) protein hydrolysate and peptide fractions. Food Chemistry, 173, 652-659
Girgih Abraham T., Udenigwe Chibuike C., Aluko Rotimi E. (2011): In Vitro Antioxidant Properties of Hemp Seed (Cannabis sativa L.) Protein Hydrolysate Fractions. Journal of the American Oil Chemists' Society, 88, 381-389
Gülçin İlhami, Topal Fevzi, Çakmakçı Ramazan, Bilsel Mine, Gören Ahmet C., Erdogan Ummugulsum (2011): Pomological Features, Nutritional Quality, Polyphenol Content Analysis, and Antioxidant Properties of Domesticated and 3 Wild Ecotype Forms of Raspberries (Rubus idaeus L.). Journal of Food Science, 76, C585-C593
Hernández-Ledesma Blanca, Dávalos Alberto, Bartolomé Begoña, Amigo Lourdes (2005): Preparation of Antioxidant Enzymatic Hydrolysates from α-Lactalbumin and β-Lactoglobulin. Identification of Active Peptides by HPLC-MS/MS. Journal of Agricultural and Food Chemistry, 53, 588-593
Huang Xiaoqin, Tu Zongcai, Jiang Ying, Xiao Hui, Zhang Qiuting, Wang Hui (2012): Dynamic high pressure microfluidization-assisted extraction and antioxidant activities of lentinan. International Journal of Biological Macromolecules, 51, 926-932
Kim Se-Kwon, Wijesekara Isuru (2010): Development and biological activities of marine-derived bioactive peptides: A review. Journal of Functional Foods, 2, 1-9
Ko S.C., Kim D.G., Han C.H., Lee Y.J., Lee J.K., Byun H.G., Lee S.C., Park S.J., Lee D.H., Jeon Y.J. (2012a): Nitric oxide-mediated vasorelaxation effects of anti-angiotensin I-converting enzyme (ACE) peptide from Styela clava flesh tissue and its anti-hypertensive effect in spontaneously hypertensive rats. Food Chemistry, 134: 1141–1145.
Ko S.C., Kim D., Jeon Y.J. (2012b): Protective effect of a novel antioxidative peptide purified from a marine Chlorella ellipsoidea protein against free radical-induced oxidative stress. Food and Chemical Toxicology, 50: 2294–2302.
Li Yanhong, Jiang Bo, Zhang Tao, Mu Wanmeng, Liu Jian (2008): Antioxidant and free radical-scavenging activities of chickpea protein hydrolysate (CPH). Food Chemistry, 106, 444-450
Liu Qian, Kong Baohua, Xiong Youling L., Xia Xiufang (2010): Antioxidant activity and functional properties of porcine plasma protein hydrolysate as influenced by the degree of hydrolysis. Food Chemistry, 118, 403-410
McCann K.B., Shiell B.J., Michalski W.P., Lee A., Wan J., Roginski H., Coventry M.J. (2006): Isolation and characterisation of a novel antibacterial peptide from bovine αS1-casein. International Dairy Journal, 16, 316-323
Mendis Eresha, Rajapakse Niranjan, Kim Se-Kwon (2005): Antioxidant Properties of a Radical-Scavenging Peptide Purified from Enzymatically Prepared Fish Skin Gelatin Hydrolysate. Journal of Agricultural and Food Chemistry, 53, 581-587
Moosmann B. (): Secretory Peptide Hormones Are Biochemical Antioxidants: Structure-Activity Relationship. Molecular Pharmacology, 61, 260-268
Ngo Dai-Hung, Qian Zhong-Ji, Ryu BoMi, Park Jae W., Kim Se-Kwon (2010): In vitro antioxidant activity of a peptide isolated from Nile tilapia (Oreochromis niloticus) scale gelatin in free radical-mediated oxidative systems. Journal of Functional Foods, 2, 107-117
Pownall Trisha L., Udenigwe Chibuike C., Aluko Rotimi E. (2010): Amino Acid Composition and Antioxidant Properties of Pea Seed (Pisum sativum L.) Enzymatic Protein Hydrolysate Fractions. Journal of Agricultural and Food Chemistry, 58, 4712-4718
Qian Zhong-Ji, Jung Won-Kyo, Byun Hee-Guk, Kim Se-Kwon (2008): Protective effect of an antioxidative peptide purified from gastrointestinal digests of oyster, Crassostrea gigas against free radical induced DNA damage. Bioresource Technology, 99, 3365-3371
Rajapakse N., Mendis E., Jung W.K., Je J.Y., Kim S.K. (2005a): Purification of a radical scavenging peptide from fermented mussel sauce and its antioxidant properties. Food Research International, 38: 175–182.
Rajapakse N., Mendis E., Byun H.G., Kim S.K. (2005b): Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical-mediated oxidative systems. Journal of Nutritional Biochemistry, 16: 562–569.
Salim Samina, Asghar Mohammad, Chugh Gaurav, Taneja Manish, Xia Zhilian, Saha Kaustav (2010): Oxidative stress: A potential recipe for anxiety, hypertension and insulin resistance. Brain Research, 1359, 178-185
Sarmadi Bahareh H., Ismail Amin (2010): Antioxidative peptides from food proteins: A review. Peptides, 31, 1949-1956
Sun Yuanxia, Hayakawa Shigeru, Ogawa Masahiro, Naknukool Supaporn, Guan Yupin, Matsumoto Yoshiyuki (2011): Evaluation of angiotensin I-converting enzyme (ACE) inhibitory activities of hydrolysates generated from byproducts of freshwater clam. Food Science and Biotechnology, 20, 303-310
Taheri Ali, Sabeena Farvin K.H., Jacobsen Charlotte, Baron Caroline P. (2014): Antioxidant activities and functional properties of protein and peptide fractions isolated from salted herring brine. Food Chemistry, 142, 318-326
Tanzadehpanah Hamid, Asoodeh Ahmad, Chamani Jamshidkhan (2012): An antioxidant peptide derived from Ostrich (Struthio camelus) egg white protein hydrolysates. Food Research International, 49, 105-111
Thana Praiya, Machmudah Siti, Goto Motonobu, Sasaki Mitsuru, Pavasant Prasert, Shotipruk Artiwan (2008): Response surface methodology to supercritical carbon dioxide extraction of astaxanthin from Haematococcus pluvialis. Bioresource Technology, 99, 3110-3115
Udenigwe Chibuike C., Aluko Rotimi E. (2011): Chemometric Analysis of the Amino Acid Requirements of Antioxidant Food Protein Hydrolysates. International Journal of Molecular Sciences, 12, 3148-3161
Xia Yichen, Bamdad Fatemeh, Gänzle Michael, Chen Lingyun (2012): Fractionation and characterization of antioxidant peptides derived from barley glutelin by enzymatic hydrolysis. Food Chemistry, 134, 1509-1518
Xie Zhengjun, Huang Junrong, Xu Xueming, Jin Zhengyu (2008): Antioxidant activity of peptides isolated from alfalfa leaf protein hydrolysate. Food Chemistry, 111, 370-376
Yang Yun-Ji, Kim Se-Kwon, Park Sun-Joo (2012): An Anti-inflammatory Peptide Isolated from Seahorse Hippocampus kuda bleeler Inhibits the Invasive Potential of MG-63 Osteosarcoma Cells. Fisheries and aquatic sciences, 15, 29-36
Yong Samuel H., Karel Marcus (1978): Reaction of histidine with methyl linoleate: Characterization of the histidine degradation products. Journal of the American Oil Chemists’ Society, 55, 352-357
You Lijun, Zhao Mouming, Cui Chun, Zhao Haifeng, Yang Bao (2009): Effect of degree of hydrolysis on the antioxidant activity of loach (Misgurnus anguillicaudatus) protein hydrolysates. Innovative Food Science & Emerging Technologies, 10, 235-240
You Lijun, Zhao Mouming, Regenstein Joe M., Ren Jiaoyan (2011): In vitro antioxidant activity and in vivo anti-fatigue effect of loach (Misgurnus anguillicaudatus) peptides prepared by papain digestion. Food Chemistry, 124, 188-194
Zhang H., Jiang Q.X., Xu Y.S., Xia W.S. (2012): The nutritive composition analysis and evaluation of the by-products from freshwater mussels after pearl-fishing. Science and Technology of Food Industry. doi: 10.13386/j.ISSN:1002-0306.2012.19.069.
Zhang Huan, Xia Wen-shui, Xu Yan-shun, Jiang Qi-xing, Wang Cai-xia, Wang Wen-jie (2013): Effects of spray-drying operational parameters on the quality of freshwater mussel powder. Food and Bioproducts Processing, 91, 242-248
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