Cassava roots: perspectives of a traditional staple for bio-solvents production Séibou T.M., Rychtera M., Melzoch K. (2015): Cassava roots: perspectives of a traditional staple for bio-solvents production. Czech J. Food Sci., 33: 186-194.
download PDF
The culture media prepared from mashed cassava and cassava starch were used to test their convertibility into organic solvents such as acetone, butanol, and ethanol. The trials were conducted in Erlenmeyer flasks containing various culture media with initial starch concentrations ranging from 30 g/l to 100 g/l. Fermentation was carried out by Clostridium acetobutylicum DSM 1731. The purpose of these tests was to determine the initial starch concentration which could be used as a starting point for the fermentation in laboratory bioreactors. In this case, we used two culture media: the first one made with cassava starch (M1), and the second one with mashed fresh cassava (M2). The culture media were supplemented with organic and inorganic nutrients. Fermentation in M1 medium resulted in the production of 17.8 g/l solvents, i.e. 12.3 g/l butanol, 3.7 g/l acetone, and 1.8 g/l ethanol, while in M2 it provided 16.2 g/l solvents representing approximately 11.4 g/l of butanol, 3.0 g/l of acetone, and 1.8 g/l of ethanol. The optimum production of butanol in M1 was 12.3 g/l in 96 h of fermentation, which is a low yield of 25% and 0.13 g/l/h productivity. Although the fermentation in medium M2 was also low (with 11.4 g/l butanol optimally), it allowed to save on the nutrient supply since it was done without the addition of minerals and organic substances.
Ajao K.R., Adegun I.K. (2009): Performance evaluation of a locally fabricated mini cassava flashes dryer. Journal of Agricultural Technology, 5: 281–289.
Al-Shorgani Najeeb Kaid Nasser, Ali Ehsan, Kalil Mohd Sahaid, Yusoff Wan Mohtar Wan (2012): Bioconversion of Butyric Acid to Butanol by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) in a Limited Nutrient Medium. BioEnergy Research, 5, 287-293
Al-Shorgani Najeeb Kaid Nasser, Kalil Mohd Sahaid, Yusoff Wan Mohtar Wan (2012): Fermentation of sago starch to biobutanol in a batch culture using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). Annals of Microbiology, 62, 1059-1070
Chen C.-K., Blaschek H. P. (1999): Acetate enhances solvent production and prevents degeneration in Clostridium beijerinckii BA101. Applied Microbiology and Biotechnology, 52, 170-173
Davídek J. et al. (1977): Laboratorní příručka analýzy potravin. Prague, SNTL: 239–241
Dürre Peter (2008): Fermentative Butanol Production. Annals of the New York Academy of Sciences, 1125, 353-362
FAO (2008): The state of food and agriculture 2008. Biofuels: prospects, risks and opportunities. Roma, FAO.
Gheshlaghi R., Scharer J.M., Moo-Young M., Chou C.P. (2009): Metabolic pathways of clostridia for producing butanol. Biotechnology Advances, 27, 764-781
Green Edward M (2011): Fermentative production of butanol—the industrial perspective. Current Opinion in Biotechnology, 22, 337-343
Gunorubon A.J. (2012): Production of cassava starch-based adhesive. Research Journal in Engineering and Applied Sciences, 1: 219–214.
Jones D.T., Woods D.R. (1986): Acetone-butanol fermentation revisited. Microbiology Reviews, 50: 484–524.
Jones S. W., Tracy B. P., Gaida S. M., Papoutsakis E. T. (): Inactivation of  F in Clostridium acetobutylicum ATCC 824 Blocks Sporulation Prior to Asymmetric Division and Abolishes  E and  G Protein Expression but Does Not Block Solvent Formation. Journal of Bacteriology, 193, 2429-2440
Li Han-guang, Luo Wei, Wang Qiang, Yu Xiao-bin (2014): Direct Fermentation of Gelatinized Cassava Starch to Acetone, Butanol, and Ethanol Using Clostridium acetobutylicum Mutant Obtained by Atmospheric and Room Temperature Plasma. Applied Biochemistry and Biotechnology, 172, 3330-3341
Liew S.T, Arbakariya A., Rosfarizan M., Raha A.R (2006): Production of solvent (acetone-butanol-ethanol) in continuous fermentation by C. saccharobutylicum DSM 13864 using gelatinised sago starch as a carbon source. Malaysian Journal of Microbiology, 2: 42–50.
Linhová M. (2011): Mikrobiální produkce 1-butanolu. Bioprospect, 21: 78–84.
Lütke-Eversloh Tina, Bahl Hubert (2011): Metabolic engineering of Clostridium acetobutylicum: recent advances to improve butanol production. Current Opinion in Biotechnology, 22, 634-647
Nolling J., Breton G., Omelchenko M. V., Makarova K. S., Zeng Q., Gibson R., Lee H. M., Dubois J., Qiu D., Hitti J., GTC Sequencing Center Production , Finishing, and Bioinformatics Teams , Wolf Y. I., Tatusov R. L., Sabathe F., Doucette-Stamm L., Soucaille P., Daly M. J., Bennett G. N., Koonin E. V., Smith D. R. (2001): Genome Sequence and Comparative Analysis of the Solvent-Producing Bacterium Clostridium acetobutylicum. Journal of Bacteriology, 183, 4823-4838
Ramey D.E, Shang-Tian Y. (2004): Production of butyric acid and butanol from biomass. Final Report. Work Performed under Contract No. DE-F-G02-00ER86106. Morgantown, U.S. Department of Energy.
Shaheen R., Shirley M., Jones D.T. (2000): Comparative fermentation studies of industrial strains belonging to four species of solvent-producing clostridia. Journal of Molecular Microbiology and Biotechnology, 2: 115–124.
Yang G., Shiyuan H., Jun C., Lijun S., Huiqi H., Yunliu Y., Sheng Y., Weihong J. (2009): Ammonium acetate enhances solvent production by C. acetobutylicum EA 2018 using cassava as a fermentation medium. Journal of Industrial Microbiology & Biotechnology, 36: 1225–1232.
Li Zhigang, Shi Zhongping, Li Xin, Li Le, Zheng Junping, Wang Zhenggang (2013): Evaluation of high butanol/acetone ratios in ABE fermentations with cassava by graph theory and NADH regeneration analysis. Biotechnology and Bioprocess Engineering, 18, 759-769
Zverlov V. V., Berezina O., Velikodvorskaya G. A., Schwarz W. H. (2006): Bacterial acetone and butanol production by industrial fermentation in the Soviet Union: use of hydrolyzed agricultural waste for biorefinery. Applied Microbiology and Biotechnology, 71, 587-597
download PDF

© 2021 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti