Estimation of the antioxidant properties of milk protein preparations hydrolyzed by Lactobacillus helveticus T80, T105 and B734 K., Sołowiej B., Waśko A., Kononiuk A., Gustaw W. (2019): Estimation of the antioxidant properties of milk protein preparations hydrolyzed by Lactobacillus helveticus T80, T105 and B734. Czech J. Food Sci., 37: 260-267.
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The investigation determined the influence of Lactobacillus helveticus strains (T80, T105, B734 and DSMZ 20075) and Lactobacillus acidophilus La-05 (probiotic strain) on the antioxidant activity of whey and milk protein preparations. In order to indicate possible mechanism of antioxidant activity of the obtained hydrolysates, the reducing power (RP) assay, ability to neutralize free radicals and Fe2+ chelating activity analysis were employed. The results suggest that antioxidant activity of tested hydrolysates depends on the type of protein preparation (substrate) and the applied bacterial strain. The strongest antioxidant activity was exhibited by whey protein isolate hydrolysate obtained by using L. helveticus T80. A majority of fermented products obtained with the use of the probiotic strain were characterized by higher antioxidant properties than those obtained by application of L. helveticus. The solution of caseinoglicomacropeptide fermented by strain T105 exhibited the highest RP values, while α-lactalbumin hydrolysed by probiotic strain (La-5) was characterized by the strongest Fe2+ chelating activity. The analysed protein preparations and their hydrolysates obtained with using tested bacteria might potentially be applied in food products in order to inhibit oxidation processes.

Abubakr M.A.S., Hassan Z., Eswai G.S.M. (2012): Evaluation of antioxidant activity of skim milk hydrolysate fermented with lactic acid bacteria. Acta Biologica Malayasiana, 1: 85–93.
Beganović Jasna, Kos Blaženka, Leboš Pavunc Andreja, Uroić Ksenija, Džidara Petra, Šušković Jagoda (2013): Proteolytic activity of probiotic strain Lactobacillus helveticus M92. Anaerobe, 20, 58-64
Bradford Marion M. (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254
Cho Young-Hee, Shin Il-Seung, Hong Sung-Moon, Kim Cheol-Hyun (2015): Production of Functional High-protein Beverage Fermented with Lactic Acid Bacteria Isolated from Korean Traditional Fermented Food. Korean Journal for Food Science of Animal Resources, 35, 189-196
Conway Valérie, Gauthier Sylvie F., Pouliot Yves (2013): Antioxidant Activities of Buttermilk Proteins, Whey Proteins, and Their Enzymatic Hydrolysates. Journal of Agricultural and Food Chemistry, 61, 364-372
Corrêa Ana Paula F, Daroit Daniel J, Coelho Julise, Meira Stela MM, Lopes Fernanda C, Segalin Jéferson, Risso Patrícia H, Brandelli Adriano (2011): Antioxidant, antihypertensive and antimicrobial properties of ovine milk caseinate hydrolyzed with a microbial protease. Journal of the Science of Food and Agriculture, , n/a-n/a
Corrêa Ana Paula Folmer, Daroit Daniel Joner, Fontoura Roberta, Meira Stela Maris Meister, Segalin Jeferson, Brandelli Adriano (2014): Hydrolysates of sheep cheese whey as a source of bioactive peptides with antioxidant and angiotensin-converting enzyme inhibitory activities. Peptides, 61, 48-55
Elfahri K.R., Vasiljevic T., Yeager T., Donkor O.N. (2016): Anti-colon cancer and antioxidant activities of bovine skim milk fermented by selected Lactobacillus helveticus strains. Journal of Dairy Science, 99, 31-40
El-Fattah Alaa Abd, Sakr Sally, El-Dieb Samia, Elkashef Hany (2016): Angiotensin-converting enzyme inhibition and antioxidant activity of commercial dairy starter cultures. Food Science and Biotechnology, 25, 1745-1751
Griffiths M. W., Tellez A. M. (2013): Lactobacillus helveticus: the proteolytic system. Frontiers in Microbiology, 4, -
Jeewanthi Renda Kankanamge Chaturika, Lee Na-Kyoung, Paik Hyun-Dong (2015): Improved Functional Characteristics of Whey Protein Hydrolysates in Food Industry. Korean Journal for Food Science of Animal Resources, 35, 350-359
Jensen Marie P., Vogensen Finn K., Ardö Ylva (2009): Variation in caseinolytic properties of six cheese related Lactobacillus helveticus strains. International Dairy Journal, 19, 661-668
Khantaphant Sutheera, Benjakul Soottawat, Kishimura Hideki (2011): Antioxidative and ACE inhibitory activities of protein hydrolysates from the muscle of brownstripe red snapper prepared using pyloric caeca and commercial proteases. Process Biochemistry, 46, 318-327
Korhonen Hannu, Pihlanto Anne (2006): Bioactive peptides: Production and functionality. International Dairy Journal, 16, 945-960
LAEMMLI U. K. (1970): Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature, 227, 680-685
Li Jie, O Wuliji, Li Wei, Jiang Zhi-Gang, Ghanbari Hossein (2013): Oxidative Stress and Neurodegenerative Disorders. International Journal of Molecular Sciences, 14, 24438-24475
Mahomud Md. Sultan, Katsuno Nakako, Nishizu Takahisa (2017): ROLE OF WHEY PROTEIN-CASEIN COMPLEXES ON YOGHURT TEXTURE. Reviews in Agricultural Science, 5, 1-12
Namdari A., Nejati F. (2016): Development of antioxidant activity during milk fermentation by wild isolates of Lactobacillus helveticus. Applied Food Biotechnology, 3: 178–187.
Niedzielska Ewa, Smaga Irena, Gawlik Maciej, Moniczewski Andrzej, Stankowicz Piotr, Pera Joanna, Filip Małgorzata (2016): Oxidative Stress in Neurodegenerative Diseases. Molecular Neurobiology, 53, 4094-4125
Osuntoki A., Korie I. (2010): Antioxidant activity whey from milk fermented with Lactobacillus species isolated from Nigerian fermented foods. Food Technology and Biotechnology, 48: 505–511.
Ozcan T., Yilmaz-Ersan Y., Akpinar-Bayizit A., Delikanli B. (2016): Antioxidant properties of probiotic fermented milk supplemented with chestnut flour (Castanea sativa Mill). Journal of Food Processing and Preservation, 41: 1–9.
Phelan Martha, Aherne Aisling, FitzGerald Richard J., O'Brien Nora M. (2009): Casein-derived bioactive peptides: Biological effects, industrial uses, safety aspects and regulatory status. International Dairy Journal, 19, 643-654
Pihlanto Anne (2006): Antioxidative peptides derived from milk proteins. International Dairy Journal, 16, 1306-1314
Praveesh B.V., Angayarkanni J., Palaniswamy M. (2013): Therapeutical properties of cow milk fermented with Lactobacillus plantarum and Lactococcus casei. African Journal of Biotechnology, 12: 3296–3301.
Rashad M., Abeer E., Hala M., Mohamed U. (2011): Improvement of nutritional quality and antioxidant activities of yeast fermented soybean curd residue. African Journal of Biotechnology, 10: 5504–5513.
Ríos-Arrabal Sandra, Artacho-Cordón Francisco, León Josefa, Román-Marinetto Elisa, del Mar Salinas-Asensio María, Calvente Irene, Núñez Maria Isabel (2013): Involvement of free radicals in breast cancer. SpringerPlus, 2, 404-
Rossini Karina, Noreña Caciano P.Z., Cladera-Olivera Florencia, Brandelli Adriano (2009): Casein peptides with inhibitory activity on lipid oxidation in beef homogenates and mechanically deboned poultry meat. LWT - Food Science and Technology, 42, 862-867
Sadat-Mekmene L., Jardin J., Corre C., Mollé D., Richoux R., Delage M.-M., Lortal S., Gagnaire V. (2010): Simultaneous Presence of PrtH and PrtH2 Proteinases in Lactobacillus helveticus Strains Improves Breakdown of the Pure α s1 -Casein. Applied and Environmental Microbiology, 77, 179-186
Sadat-Mekmene L., Richoux R., Aubert-Frogerais L., Madec M.-N., Corre C., Piot M., Jardin J., le Feunteun S., Lortal S., Gagnaire V. (2013): Lactobacillus helveticus as a tool to change proteolysis and functionality in Swiss-type cheeses. Journal of Dairy Science, 96, 1455-1470
Sarmadi Bahareh H., Ismail Amin (2010): Antioxidative peptides from food proteins: A review. Peptides, 31, 1949-1956
Shiby Varghese Kochupurakkal, Radhakrishna Kolpe, Bawa Amarinder Singh (2013): Development of whey-fruit-based energy drink mixes using D-optimal mixture design. International Journal of Food Science & Technology, 48, 742-748
Skrzypczak Katarzyna W., Gustaw Waldemar Z., Jabłońska-Ryś Ewa D., Michalak-Majewska Monika, Sławińska Aneta, Radzki Wojciech P., Gustaw Klaudia M., Waśko Adam D. (2015): Antioxidative properties of milk protein preparations fermented by Polish strains of Lactobacillus helveticus. Acta Scientiarum Polonorum Technologia Alimentaria, 16, 199-207
Virtanen T., Pihlanto A., Akkanen S., Korhonen H. (2007): Development of antioxidant activity in milk whey during fermentation with lactic acid bacteria. Journal of Applied Microbiology, 102, 106-115
Waśko Adam, Szwajgier Dominik, Polak-Berecka Magdalena (2014): The role of ferulic acid esterase in the growth of Lactobacillus helveticus in the presence of phenolic acids and their derivatives. European Food Research and Technology, 238, 299-306
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