Use of an exogenous carboxypeptidase to accelerate proteolysis in Reggianito cheese

https://doi.org/10.17221/567/2015-CJFSCitation:J Ceruti R., B Pirola M., Ramos E., Robert L., C Rubiolo A., A Sihufe G. (2016): Use of an exogenous carboxypeptidase to accelerate proteolysis in Reggianito cheese. Czech J. Food Sci., 34: 445-455.
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The effect of an exogenous commercial carboxypeptidase on the proteolysis of Reggianito cheese was evaluated. Cheeses were manufactured using 4 concentrations of enzyme: 0, 5, 10, and 20 g/100 l milk. Cheese samples were analysed at 0, 60, 90, 120, 150, 180, and 210 days of ripening. Nitrogen content values increased during ripening, but no clear effect due to the enzyme addition was observed. A profound degradation of β-casein was observed during the first 2 months of ripening in cheeses with the highest rates of enzyme addition. An increase of amino acid concentrations was clearly observed in some cheeses manufactured with exogenous enzymes compared with control cheeses at the same ripening time. However, the principal component analysis showed that experimental cheeses had a slight increase in the rate of formation and/or degradation of proteolysis products. Our results represent an important contribution to select new alternatives for enzyme addition during the manufacture of hard cheese.
References:
Azarnia Sorayya, Robert Normand, Lee Byong (2006): Biotechnological Methods to Accelerate Cheddar Cheese Ripening. Critical Reviews in Biotechnology, 26, 121-143  https://doi.org/10.1080/07388550600840525
 
Azarnia Sorayya, Lee Byong H., Yaylayan Varoujan, Kilcawley Kieran N. (2010): Proteolysis development in enzyme-modified Cheddar cheese using natural and recombinant enzymes of Lactobacillus rhamnosus S93. Food Chemistry, 120, 174-178  https://doi.org/10.1016/j.foodchem.2009.10.003
 
Azarnia Sorayya, Lee Byong, St-Gelais Daniel, Kilcawley Kieran, Noroozi Ebrahim (2011): Effect of free and encapsulated recombinant aminopeptidase on proteolytic indices and sensory characteristics of Cheddar cheese. LWT - Food Science and Technology, 44, 570-575  https://doi.org/10.1016/j.lwt.2010.08.022
 
Candioti Mario César, Hynes Erica, Quiberoni Andrea, Palma Susana Beatriz, Sabbag Nora, Zalazar Carlos Antonio (2002): Reggianito Argentino cheese: influence of Lactobacillus helveticus strains isolated from natural whey cultures on cheese making and ripening processes. International Dairy Journal, 12, 923-931  https://doi.org/10.1016/S0958-6946(02)00115-2
 
Doolan I.A., Nongonierma A.B., Kilcawley K.N., Wilkinson M.G. (2014): Partitioning of starter bacteria and added exogenous enzyme activities between curd and whey during Cheddar cheese manufacture. International Dairy Journal, 34, 159-166  https://doi.org/10.1016/j.idairyj.2013.07.005
 
El Soda M., Awad S. (2011): Acelerated cheese ripening. In: Fuquay J.W., Fox P.F., McSweeney P.L.H. (eds): Encyclopedia of Dairy Sciences. Vol. 1. London, Elsevier Academic Press: 795–799.
 
Forde‡ Amanda, Fitzgerald†‡ Gerald F (2000): Biotechnological approaches to the understanding and improvement of mature cheese flavour. Current Opinion in Biotechnology, 11, 484-489  https://doi.org/10.1016/S0958-1669(00)00130-0
 
Gallino R.R. (1994): Queso Reggianito Argentino: tecnología de fabricación. In: Ciencia y Tecnología de los Productos Lácteos. Diagramma S.A., Medios Audiovisuales y Gráficos – CERIDE, Santa Fe: 244–287.
 
Gobbetti M., Burzigotti R., Smacchi E., Corsetti A., De Angelis M. (1997): Microbiology and biochemistry of gorgonzola cheese during ripening. International Dairy Journal, 7, 519-529  https://doi.org/10.1016/S0958-6946(97)00047-2
 
GRIPON J. C., DESMAZEAUD M. J., LE BARS D., BERGERE J. L. (1975): Etude du rôle des micro-organismes et des enzymes au cours de la maturation des fromages. II. - Influence de la présure commerciale. Le Lait, 55, 502-516  https://doi.org/10.1051/lait:197554828
 
Hynes E.R., Bergamini C.V., Suárez V.B., Zalazar C.A. (2003): Proteolysis on Reggianito Argentino Cheeses Manufactured with Natural Whey Cultures and Selected Strains of Lactobacillus helveticus. Journal of Dairy Science, 86, 3831-3840  https://doi.org/10.3168/jds.S0022-0302(03)73990-3
 
Jones Barry N., Pääbo Svante, Stein Stanley (1981): Amino Acid Analysis and Enzymatic Sequence Determination of Peptides by an Improved o -Phthaldialdehyde Precolumn Labeling Procedure. Journal of Liquid Chromatography, 4, 565-586  https://doi.org/10.1080/01483918108059956
 
Kilcawley K.N, Wilkinson M.G, Fox P.F (2002): Determination of key enzyme activities in commercial peptidase and lipase preparations from microbial or animal sources. Enzyme and Microbial Technology, 31, 310-320  https://doi.org/10.1016/S0141-0229(02)00136-9
 
Kilcawley K.N., Nongonierma A.B., Hannon J.A., Doolan I.A., Wilkinson M.G. (2012): Evaluation of commercial enzyme systems to accelerate Cheddar cheese ripening. International Dairy Journal, 26, 50-57  https://doi.org/10.1016/j.idairyj.2012.03.015
 
Marcos A., Esteban M.A., León F., Fernández-Salguero J. (1979): Electrophoretic Patterns of European Cheeses: Comparison and Quantitation. Journal of Dairy Science, 62, 892-900  https://doi.org/10.3168/jds.S0022-0302(79)83345-7
 
McSweeney P.L.H. (2004): Biochemistry of cheese ripening: introduction an overview. In: Fox P.F., McSweeney P.L.H., Cogan T.M., Guinee T.P. (eds): Cheese: Chemistry, Physics and Microbiology. Vol. 1. 3rd Ed. London, Elsevier Academic Press: 347–360.
 
McSweeney P. L.H., Fox P. F. (1997): Chemical methods for the characterization of proteolysis in cheese during ripening. Le Lait, 77, 41-76  https://doi.org/10.1051/lait:199713
 
McSweeney Paul L. H., Pochet Sylvie, Fox Patrick F., Healy Aine (1994): Partial identification of peptides from the water-insoluble fraction of Cheddar cheese. Journal of Dairy Research, 61, 587-  https://doi.org/10.1017/S0022029900028533
 
Mohedano A.F, Fernández J, Garde S, Medina M, Gaya P, Nuñez M (1998): The Effect of the Cysteine Proteinase from Micrococcus sp. INIA 528 on the Ripening Process of Manchego Cheese. Enzyme and Microbial Technology, 22, 391-396  https://doi.org/10.1016/S0141-0229(97)00211-1
 
Perotti M C, Bernal S M, Meinardi C A, Candioti M C, Zalazar C A (2004): Substitution of natural whey starter by mixed strains of Lactobacillus helveticus in the production of Reggianito Argentino cheese. International Journal of Dairy Technology, 57, 45-51  https://doi.org/10.1111/j.1471-0307.2004.00128.x
 
Pripp A.H, Shakeel-Ur-Rehman , McSweeney P.L.H, Fox P.F (1999): Multivariate statistical analysis of peptide profiles and free amino acids to evaluate effects of single-strain starters on proteolysis in miniature Cheddar-type cheeses. International Dairy Journal, 9, 473-479  https://doi.org/10.1016/S0958-6946(99)00116-8
 
Reinheimer J.A., Quiberoni A., Tailliez P., Binetti A.G., Suárez V.B. (1996): The lactic acid microflora of natural whey starters used in Argentina for hard cheese production. International Dairy Journal, 6, 869-879  https://doi.org/10.1016/0958-6946(96)00014-3
 
Sihufe G.A., Zorrilla S.E., Rubiolo A.C. (2003): Casein Degradation of Fynbo Cheese Salted with NaCl/KCl Brine and Ripened at Various Temperatures. Journal of Food Science, 68, 117-123  https://doi.org/10.1111/j.1365-2621.2003.tb14125.x
 
Sihufe Guillermo A., Zorrilla Susana E., Mercanti Diego J., Perotti María C., Zalazar Carlos A., Rubiolo Amelia C. (2007): The influence of ripening temperature and sampling site on the lipolysis in Reggianito Argentino cheese. Food Research International, 40, 1220-1226  https://doi.org/10.1016/j.foodres.2007.07.003
 
Sihufe G.A., Zorrilla S.E., Rubiolo A.C. (2010a): The influence of ripening temperature and sampling site on the proteolysis in Reggianito Argentino cheese. LWT-Food Science and Technology, 43: 247–253.
 
Sihufe G.A., Zorrilla S.E., Sabbag N., Costa S., Rubiolo A.C. (2010b): The influence of ripening temperature on the sensory characteristics of Reggianito Argentino cheese. Journal of Sensory Studies, 25: 94–107.
 
Sihufe G.A., Zorrilla S.E., Wolf I.V., Perotti M.C., Zalazar C.A., Sabbag N., Costa S., Rubiolo A.C. (2010c): The acceleration of cheese ripening at elevated temperature. An estimation of the optimal ripening time of a traditional Argentinean hard cheese. Food Chemistry, 119: 101–107.
 
Sihufe G.A., Rubiolo A.C., Zorrilla S.E. (2012): Reggianito cheese: hard cheese produced in Argentina. In: Hui Y.H. (ed.): Handbook of Animal-based Fermented Food and Beverage Technology. 2nd Ed. Boca Raton, CRC Press: 377–386.
 
Simal S., Sánchez E.S., Bon J., Femenia A., Rosselló C. (2001): Water and salt diffusion during cheese ripening: effect of the external and internal resistances to mass transfer. Journal of Food Engineering, 48, 269-275  https://doi.org/10.1016/S0260-8774(00)00169-2
 
Upadhyay V.K., McSweeney P.L.H. (2003): Acceleration of cheese ripening. In: Smit G. (ed.): Dairy Processing: Improving Quality. Boca Raton, CRC Press: 419–447.
 
Wilkinson M.G., Kilcawley K.N. (2005): Mechanisms of incorporation and release of enzymes into cheese during ripening. International Dairy Journal, 15, 817-830  https://doi.org/10.1016/j.idairyj.2004.08.021
 
Zalazar C., Meinardi C., Hynes E. (1999): Quesos típicos argentinos. Una revisión general sobre producción y características. Santa Fe, Centro de Publicaciones de la Universidad Nacional del Litoral: 20–49.
 
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