Food safety and label claims for hazelnut allergy traces: evaluation of two PCR assays
D.P. Houhoula, K. Lagou, M. Varvaresou, M. Giannakourou, M. Bratakos S, V. Lougovois, J. Tsaknis, S. Koussissishttps://doi.org/10.17221/602/2014-CJFSCitation:Houhoula D.P., Lagou K., Varvaresou M., Giannakourou M., Bratakos S M., Lougovois V., Tsaknis J., Koussissis S. (2015): Food safety and label claims for hazelnut allergy traces: evaluation of two PCR assays. Czech J. Food Sci., 33: 410-415.
The molecular techniques (C-PCR, RT-PCR) in the detection and quantification of allergic substances of hazelnut in various categories of food commodities, e.g. breakfast cereals, chocolates and biscuits, frequently involved in allergic outbreaks was implemented. For the detection of hazelnut a gene coding the major allergenic protein Cor a1 was selected. In some instances, the presence of hazelnuts is not declared on the label and the products may carry no warning for potentially allergenic substances, usually referred to as “traces”. A total of 150 samples were collected from local supermarkets and analysed for the purpose of the study. From these, a total of 38 (25.3%) specimens contained hazelnut, 30 (20.0%) contained “traces” of hazelnut, 26 (17.3%) contained a label warning for the possible presence of “traces” of allergenic substances, and 56 (37.3%) specimens contained no food allergy labels. Among them, using the C-PCR, 36 (94.7%), 10 (33.3%), 5 (19.2%), and 5 (8.9%) specimens were detected as positive, respectively. Using the RT-PCR, 38 (100%), 15 (50%), 7 (26.9%) and 8 (14.3%) specimens were detected as positive, respectively. Finally, by combining both methods, 38 (100%), 17 (56.7%), 9 (34.6%), and 10 (17.9%) specimens were identified as positive, respectively.Keywords:amplification; declaration; food allergy; hazelnutReferences:
Arlorio M., Cereti E., Coïsson J.D., Travaglia F., Martelli A. (2007): Detection of hazelnut (Corylus spp.) in processed foods using real-time PCR. Food Control, 18, 140-148 https://doi.org/10.1016/j.foodcont.2005.09.005Crespo Jesus F., James John M., Fernandez-Rodriguez Consuelo, Rodriguez Julia (2006): Food allergy: nuts and tree nuts. British Journal of Nutrition, 96, S95- https://doi.org/10.1017/BJN20061869Ehlert Alexandra, Demmel Anja, Hupfer Christine, Busch Ulrich, Engel Karl-Heinz (2009): Simultaneous detection of DNA from 10 food allergens by ligation-dependent probe amplification. Food Additives & Contaminants: Part A, 26, 409-418 https://doi.org/10.1080/02652030802593529Herman L., Block J.D., Viane R. (2003): Detection of hazelnut DNA traces in chocolate by PCR. International Journal of Food Science & Technology, 38: 633–640.Hirschwehr Reinhold, Valenta Rudolf, Ebner Christof, Ferreira Fatima, Sperr Wolfgang R., Valent Peter, Rohac Madeleine, Rumpold Helmut, Scheiner Otto, Kraft Dietrich (1992): Identification of common allergenic structures in hazel pollen and hazelnuts: A possible explanation for sensitivity to hazelnuts in patients allergic to tree pollen. Journal of Allergy and Clinical Immunology, 90, 927-936 https://doi.org/10.1016/0091-6749(92)90465-EHolzhauser Thomas, Vieths Stefan (1999): Quantitative Sandwich ELISA for Determination of Traces of Hazelnut ( Corylus avellana ) Protein in Complex Food Matrixes. Journal of Agricultural and Food Chemistry, 47, 4209-4218 https://doi.org/10.1021/jf990478qHolzhauser Thomas, Vieths Stefan (1999): Indirect Competitive ELISA for Determination of Traces of Peanut ( Arachis hypogaea L.) Protein in Complex Food Matrices. Journal of Agricultural and Food Chemistry, 47, 603-611 https://doi.org/10.1021/jf980775fHolzhauser T., Wangorsch A., Vieths S. (2000): Polymerase chain reaction (PCR) for detection of potentially allergenic hazelnut residues in complex food matrixes. European Food Research and Technology, 211, 360-365 https://doi.org/10.1007/s002170000152Köppel René, Dvorak Veronika, Zimmerli Franziska, Breitenmoser Alda, Eugster Albert, Waiblinger Hans-Ulrich (2010): Two tetraplex real-time PCR for the detection and quantification of DNA from eight allergens in food. European Food Research and Technology, 230, 367-374 https://doi.org/10.1007/s00217-009-1164-3Koppelman Stef J, Knulst André C, Koers Willem J, Penninks André H, Peppelman Heleen, Vlooswijk Riek, Pigmans Ingrid, van Duijn Gert, Hessing Martin (1999): Comparison of different immunochemical methods for the detection and quantification of hazelnut proteins in food products. Journal of Immunological Methods, 229, 107-120 https://doi.org/10.1016/S0022-1759(99)00119-2Lidholm Jonas, Ballmer-Weber Barbara K, Mari Adriano, Vieths Stefan (2006): Component-resolved diagnostics in food allergy. Current Opinion in Allergy and Clinical Immunology, 6, 234-240 https://doi.org/10.1097/01.all.0000225166.90768.d6López-Calleja Inés María, de la Cruz Silvia, Pegels Nicolette, González Isabel, García Teresa, Martín Rosario (2013): High resolution TaqMan real-time PCR approach to detect hazelnut DNA encoding for ITS rDNA in foods. Food Chemistry, 141, 1872-1880 https://doi.org/10.1016/j.foodchem.2013.05.076Murzin Alexey G., Brenner Steven E., Hubbard Tim, Chothia Cyrus (1995): SCOP: A structural classification of proteins database for the investigation of sequences and structures. Journal of Molecular Biology, 247, 536-540 https://doi.org/10.1016/S0022-2836(05)80134-2Pastorello Elide A., Vieths Stefan, Pravettoni Valerio, Farioli Laura, Trambaioli Chiara, Fortunato Donatella, Lüttkopf Dirk, Calamari Marianna, Ansaloni Raffaella, Scibilia Joseph, Ballmer-Weber Barbara K., Poulsen Lars K., Wütrich Brunello, Hansen Kirsten Skamstrup, Robino Anna Maria, Ortolani Claudio, Conti Amedeo (2002): Identification of hazelnut major allergens in sensitive patients with positive double-blind, placebo-controlled food challenge results. Journal of Allergy and Clinical Immunology, 109, 563-570 https://doi.org/10.1067/mai.2002.121946Pedersen M.H., Holzhauser T., Bisson C., Conti A., Jensen L.B., Skov P.S., Bindslev-Jensen C., Brinch D.S., Poulsen L.K. (2008): Soybean allergen detection methods – a comparison study. Molecular Nutrition & Food Research, 52: 1486–1496.Pele M., Brohée M., Anklam E., Van Hengel A.J. (2007): Peanut and hazelnut traces in cookies and chocolates: Relationship between analytical results and declaration of food allergens on product labels. Food Additives & Contaminant, 24: 1334–1344.Piknová L., Pangallo D., Kuchta T. (2008): A novel real-time polymerase chain reaction (PCR) method for the detection of hazelnuts in food. European Food Research and Technology, 226, 1155-1158 https://doi.org/10.1007/s00217-007-0644-6Poms R.E., Klein C.L., Anklam E. (2004): Methods for allergen analysis in food: a review. Food Additives & Contaminants, 21: 1–31.Rejeb B., Abbott S., Davies M., Querry D., Cléroux J., Streng C., Delahaut C., Yeung P., Jupiter M. (2003): Immunochemical-based method for detection of hazelnut proteins in processed foods. Journal of AOAC International, 86: 557–563.Sampson Hugh A (2004): Update on food allergy☆. Journal of Allergy and Clinical Immunology, 113, 805-819 https://doi.org/10.1016/j.jaci.2004.03.014Schöringhumer Kerstin, Cichna-Markl Margit (2007): Development of a Real-Time PCR Method To Detect Potentially Allergenic Sesame ( Sesamum indicum ) in Food. Journal of Agricultural and Food Chemistry, 55, 10540-10547 https://doi.org/10.1021/jf0719407Schöringhumer Kerstin, Redl Gerda, Cichna-Markl Margit (2009): Development and Validation of a Duplex Real-Time PCR Method To Simultaneously Detect Potentially Allergenic Sesame and Hazelnut in Food. Journal of Agricultural and Food Chemistry, 57, 2126-2134 https://doi.org/10.1021/jf8033336Słowianek M., Majak I. (2011): Methods of allergen detection based on DNA analysis. Biotechnology and Food Science, 75: 39–44.Steinman Harris A. (1996): “Hidden” allergens in foods. Journal of Allergy and Clinical Immunology, 98, 241-250 https://doi.org/10.1016/S0091-6749(96)70146-X