Honey: Determination of volatile compounds, antioxidant and antibacterial activities

https://doi.org/10.17221/63/2021-CJFSCitation:

Karlidag S., Keskin M., Bayram S., Mayda N., Ozkok A. (2021): Honey: Determination of volatile compounds, antioxidant and antibacterial activities. Czech J. Food Sci., 39: 208–216.

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Honey is an important functional food for human health and nutrition that is collected by honey bees and stored in the honeycombs. In this study, total phenolic content, antioxidant activity and volatile compounds of 13 different honey samples collected from various districts of Malatya province were investigated. As a result of this study, it was determined that the total phenolic content varied between 8.50 mg GAE 100 g–1 and 73.90 mg GAE 100 g–1 and it was observed that the honey samples were rich in aldehydes, aliphatic acid and esters, alcohols, hydrocarbons, carboxylic acid esters, ketones, terpenes, fatty acids and esters. In addition, the antibacterial effects of honey samples were determined against 18 different pathogenic bacteria using agar well diffusion (AWD) method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). In AWD assay, it was recorded that inhibition zone diameters varied between 9 mm and 14 mm and honey samples were found to have a partial inhibitory effect against selected target pathogens.

References:
Al-Mamary M.A. (2002): Antioxidant activity of commonly consumed vegetables in Yemen. Malaysian Journal of Nutrition, 8: 179–189.
 
Barcarolo R., Centeleghe M., Zanatta P., Conte L.S. (1998): GC-MS coupled with head space sampling with reverse carrier flow in sampling step applied to honey characterization. In: 5th International Symposium of Hyphenated Technique in Chromatography, Bruges, Belgium, Feb 1, 1998: 11–21.
 
Benzie I.F., Strain J.J. (1999): Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology, 299: 15–27.
 
Bobiş O., Mărghitaş L.A., Dezmirean D., Gherman B., Chirilă F. (2013): In vitro antibacterial activity of unifloral honeys against honeybee pathogens Paenibacillus larvae and Escherichia coli. Lucrări Stiinţifice – Seria Zootehnie, 55: 160.
 
Dezmirean D., Mărghita, L.A., Fiţ N., Chirilă F., Gherman B., Mărgăoan R., Aurori A., Bobiş O. (2015): Antibacterial effect of heather honey (Calluna vulgaris) against different microorganisms of clinical importance. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca (UASVM), Animal Science and Biotechnologies, 72: 72–77. https://doi.org/10.15835/buasvmcn-asb:10562
 
Ecem Bayram N., Yüzer M.O., Bayram S. (2019): Melissopalynology analysis, physicochemical properties, multi-element content and antimicrobial activity of honey samples collected from Bayburt, Turkey. Uludag Bee Journal, 19: 161–176.
 
Gürbüz S., Özenirler Ç., Mayda N., Çelemli Ö.G., Özkök A. (2019): Pollen spectrum of some honey samples produced in Siirt-Turkey. Hacettepe Journal of Biology and Chemistry, 47: 295–303.
 
Huttunen S., Riihinen K., Kauhanen J., Tikkanen Kaukanen C. (2013): Antimicrobial activity of different Finnish monofloral honeys against human pathogenic bacteria. Apmis, 121: 827–834. https://doi.org/10.1111/apm.12039
 
Kassim M., Achoui M., Mustafa M.R., Mohd M.A., Yusoff K.M. (2010): Ellagic acid, phenolic acids, and flavonoids in Malaysian honey extracts demonstrate in vitro anti-inflammatory activity. Nutrition research, 30: 650–659. https://doi.org/10.1016/j.nutres.2010.08.008
 
Keskin Ş., Mayda N., Keskin M., Özkök A. (2020): Investigation of Bılecık honeys in terms of melissopalynology and chemical analyses (Bilecik ballarinin melissopalinolojik ve kimyasal özellikleri açisindan değerlendirilmesi). Food (Gıda), 45: 275–289. (in Turkish) https://doi.org/10.15237/gida.GD19107
 
Kuś P.M., Congiu F., Teper D., Sroka Z., Jerković I., Tuberoso C.I.G. (2014): Antioxidant activity, color characteristics, total phenol content and general HPLC fingerprints of six Polish unifloral honey types. LWT – Food Science and Technology, 55: 124–130. https://doi.org/10.1016/j.lwt.2013.09.016
 
Lachman J., Orsák M., Hejtmánková A., Kovářová E. (2010): Evaluation of antioxidant activity and total phenolics of selected Czech honeys. LWT – Food Science and Technology, 43: 52–58. https://doi.org/10.1016/j.lwt.2009.06.008
 
Mădaş N.M., Mărghitaş L.A., Dezmirean D.S., Bonta V., Bobiş O., Fauconnier M.L., Francis F., Haubruge E., Nguyen K.B. (2019): Volatile profile and physico-chemical analysis of acacia honey for geographical origin and nutritional value determination. Foods, 8: 445. https://doi.org/10.3390/foods8100445
 
Mayda N., Keskin M., Keskin Ş., Ozkok A. (2019): Determination of botanic origins, total phenolic and flavonoid contents of bee pollen collected from the Bilecik province (Bilecik ilinden toplanan ari polenlerinin botanik orijinleri ile toplam fenolik ve flavonoid içeriklerinin belirlenmesi). Uludag Bee Journal (Uludağ Arıcılık Dergisi), 19: 152–160. (in Turkish) https://doi.org/10.31467/uluaricilik.605692
 
Mohamed M., Sirajudeen K.N.S., Swamy M., Yaacob M., Sulaiman S. (2010): Studies on the antioxidant properties of Tualang honey of Malaysia. African Journal of Traditional, Complementary and Alternative Medicines, 7: 59–63. https://doi.org/10.4314/ajtcam.v7i1.57256
 
Osés S.M., Pascual-Mate A., de la Fuente D., de Pablo A., Fernandez-Muino M.A., Sancho M.T. (2016): Comparison of methods to determine antibacterial activity of honeys against Staphylococcus aureus. Journal of the Royal Netherlands Society for Agricultural Sciences (NJAS) – Wageningen Journal of Life Sciences, 78: 29–33. https://doi.org/10.1016/j.njas.2015.12.005
 
Pyrzynska K., Biesaga M. (2009): Analysis of phenolic acids and flavonoids in honey. TrAC – Trends in Analytical Chemistry, 28: 893–902. https://doi.org/10.1016/j.trac.2009.03.015
 
Sherlock O., Dolan A., Athman R., Power A., Gethin G., Cowman S., Humphreys H. (2010): Comparison of the antimicrobial activity of Ulmo honey from Chile and Manuka honey against methicillin-resistant Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. BioMed Central (BMC) Complementary and Alternative Medicine, 10: 1–5. https://doi.org/10.1186/1472-6882-10-47
 
Singleton V.L., Rossi J.A. (1965): Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16: 144–158.
 
Singleton V.L., Orthofer R., Lamuela-Raventós R.M. (1999): Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 299: 152–178.
 
Sorkun K. (2008): Turkey's Nectar Plants, Pollen and Honey (Türkiye'nin Nektarlı Bitkileri, Polenleri ve Balları). Ankara, Turkey, Palme Yayıncılık: 341. (in Turkish)
 
Tamas-Krumpe O., Bobıș O., Mărgăoan R., Chırılă F., Lațıu C., Ognean L. (2019): Correlative research regarding the total polyphenolic content, antioxidant and antibacterial activity of three types of Romanian honey. Scientific Papers. Series D. Animal Science, LXII: 282–288.
 
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