Effects of drying techniques on chemical composition and volatile constituents of bee pollen

https://doi.org/10.17221/79/2020-CJFSCitation:

Keskin M., Özkök A. (2020): Effects of drying techniques on chemical composition and volatile constituents of bee pollen. Czech J. Food Sci., 38: 203–208.

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Bee pollen is used as a food supplement by humans as it is rich in carbohydrates, proteins, lipids, vitamins, minerals and trace elements. Bee pollen has many biological activities such as antibacterial, antifungal, antitumor and antioxidant. Fresh bee pollen is not suitable for long-term storage because of its moisture content. In order to protect the nutrient content and freshness, the bee pollen can be dried by using different drying techniques. In this study, the biochemical characterization of the bee pollen samples dried by different techniques and drying effects on the biochemical properties of bee pollen were determined. Moisture, total lipid and protein, pH and total phenolic content of pollen samples were determined. The results ranged 6.23–20.62%, 4.98–5.57%, 16.812–1.477%, 4.08–4.33 and 15.2–22.73 mg GAE g–1, respectively. All samples are rich in squalene and methyl octadecanoate. It is clear that bee pollen bioactive components will be less damaged by using drying methods performed under more moderate conditions like lyophilization than when the traditional method is used.

References:
Bankova V., De Castro S., Marcucci M. (2000): Propolis: Recent advances in chemistry and plant origin. Apidologie, 31: 3–15. https://doi.org/10.1051/apido:2000102
 
Bogdanov, S. (2016): Pollen: Nutrition, functional properties, health (Chapter 2). In: Bogdanov, S. (ed.): The Pollen Book. Bee Product Science, Switzerland: 1–30.
 
Carpes S.T., De Alencar S.M., Cabral I.S.R., Oldoni T.L.C., Mourão G.B., Haminiuk C.W.I., Masson M.L. (2013): Polyphenols and palynological origin of bee pollen of Apis mellifera L. from Brazil. Characterization of polyphenols of bee pollen. CyTA – Journal of Food, 11: 150–161.
 
Corvucci F., Nobili L., Melucci D., Grillenzoni F.V. (2015): The discrimination of honey origin using melissopalynology and Raman spectroscopy techniques coupled with multivariate analysis. Food Chemistry, 169: 297–304. https://doi.org/10.1016/j.foodchem.2014.07.122
 
De-Melo A.A.M., Estevinho M.L.M.F., Sattler J.A.G., Souza B.R., da Silva Freitas A., Barth O.M., Almeida-Muradian L.B. (2016): Effect of processing conditions on characteristics of dehydrated bee-pollen and correlation between quality parameters. LWT – Food Science and Technology, 65: 808–815. https://doi.org/10.1016/j.lwt.2015.09.014
 
Dietz A. (1984): Nutrition of the adult honey bee. The Hive and Honey Bee. Dadant and Sons Inc., Hamilton, IL, USA: 125–156.
 
Feás X., Vázquez-Tato M.P., Estevinho L., Seijas J.A., Iglesias A. (2012): Organic bee pollen: Botanical origin, nutritional value, bioactive compounds, antioxidant activity and microbiological quality. Molecules, 17: 8359–8377. https://doi.org/10.3390/molecules17078359
 
Gaidhani K.A., Harwalkar M., Bhambere D., Nirgude P.S. (2015): Lyophilization/freeze drying – a review. World journal of pharmaceutical research, 4: 516–543.
 
Isidorov V.A., Isidorova A.G., Sczczepaniak L., Czyżewxska U. (2009): Gas chromatographic – mass spectrometric investigation of the chemical composition of beebread. Food Chemistry, 115: 1056–1063. https://doi.org/10.1016/j.foodchem.2008.12.025
 
Işık A., Ozdemir M., Doymaz I. (2019a): Infrared drying of bee pollen: effects and impacts on food components. Czech Journal of Food Sciences, 37: 69–74. https://doi.org/10.17221/410/2017-CJFS
 
Işık A., Ozdemir M., Doymaz I. (2019b): Effect of hot air drying on quality characteristics and physicochemical properties of bee pollen. Food Science and Technology, 39: 224–231. https://doi.org/10.1590/fst.02818
 
Kasper J.C., Winter G., Friess W. (2013): Recent advances and further challenges in lyophilization. European Journal of Pharmaceutics and Biopharmaceutics, 85: 162–169. https://doi.org/10.1016/j.ejpb.2013.05.019
 
Mayda N., Keskin M., Keskin Ş., Özkök, A. (2019): Determination of botanic origins, total phenolic and flavonoid contents of bee pollen collected from the Bilecik province. Uludağ Arıcılık Dergisi (Uludag Bee Journal), 19: 152–160. (In Turkish) https://doi.org/10.31467/uluaricilik.605692
 
Mayda N., Özkök A., Bayram N.E., Gerçek Y.C., Sorkun K. (2020): Bee bread and bee pollen of different plant sources: Determination of phenolic content, antioxidant activity, fatty acid and element profiles. Journal of Food Measurement and Characterization, 14: 1795–1809. https://doi.org/10.1007/s11694-020-00427-y
 
Mărgăoan R., Mărghitaş L.A., Dezmirean D., Mihai C.M., Bobiş O. (2010): Bee collected pollen – general aspects and chemical composition. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies, 67: 254–259.
 
Melo I.L.P.D., Almeida-Muradian L.B.D. (2011): Comparison of methodologies for moisture determination on dried bee pollen samples. Food Science and Technology, 31: 194–197. https://doi.org/10.1590/S0101-20612011000100029
 
Neto L., de Sousa J., Lopes J.A.D., Moita Neto J.M., Lima S.G.D., Luz C.F.P.D., Citó A.M. D.G.L. (2017): Volatile compounds and palynological analysis from pollen pots of stingless bees from the mid-north region of Brazil. Brazilian Journal of Pharmaceutical Sciences, 53: 1–9.
 
Özkök A, Sorkun K. (2006): Protein analysis with Kjeldahl of pollen grains collected by Apis mellifera L. Mellifera, 6: 7–11.
 
Özkök A, Sorkun K. (2007): The investigation of moisture and fat analysis of economically important pollen grains collected by Apis mellifera L. Mellifera, 7: 9–15.
 
Singleton V.L., Rossi J.A. (1965): Colorimetry of total phenolics with phosphomolybdic – phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16: 144–158.
 
Singleton V.L., Orthofer R., Lamuela-Raventos R.M. (1999): Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299: 152–178.
 
Silva M.D., Rosa C.I.L.F., Vilas Boas E.V.B. (2009): Control concepts and methods enzymatic browning in minimum processing of fruits and vegetables. B. Ceppa (Boletim Centro de Pesquisa de Processamento de Alimentos), 27: 83–96. (In Portuguese)
 
Sorucu A. (2019). Bee products and apitherapy. Veteriner Farmakoloji ve Toksikoloji Derneği Bülteni (Bulletin of Veterinary Pharmacology and Toxicology Association), 10: 1–15. (In Turkish)
 
Vasconcelos M.R.D.S., Duarte A.W.F., Gomes E.P., Silva S.C.D., López A.M.Q. (2017): Physicochemical composition and antioxidant potential of bee pollen from different botanical sources in Alagoas, Brazil. Ciência e Agrotecnologia, 41: 447–458. (In Portuguese) https://doi.org/10.1590/1413-70542017414009317
 
Yetim H., Kesmen Z. (2008): Food analysis. Erciyes University Publishing, Kayseri, Turkey: 163. (In Turkish)
 
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