Ultrasonic microwave-assisted extraction of polyphenols, flavonoids, triterpenoids, and vitamin C from Clinacanthus nutans  

https://doi.org/10.17221/82/2016-CJFSCitation:Yu Q., Li C., Duan Z., Liu B., Duan W., Shang F. (2017): Ultrasonic microwave-assisted extraction of polyphenols, flavonoids, triterpenoids, and vitamin C from Clinacanthus nutans  . Czech J. Food Sci., 35: 89-94.
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Polyphenols, flavonoids, triterpenoids, and vitamin C from Clinacanthus nutans were extracted by an ultrasonic microwave-assisted method. The optimal extraction conditions were established in distilled water, solid-liquid ratio 1 : 55 g/ml, irradiation power 90 W, and extraction cycle with 75 seconds. Results revealed that the solid-liquid ratio exerted a significant effect on polyphenols, whereas the irradiation power played a key role in the extraction of flavonoids, triterpenoids, and vitamin C. Under these conditions the extraction yield of each component was: polyphenols 8.893, flavonoids 25 936, triterpenoids 16 789, and vitamin C 0.166 mg/g. These results showed that the ultrasonic microwave-assisted extraction was an efficient technology to extract bioactive substances from Clinacanthus nutans and Clinacanthus nutans could potentially be a source of natural antioxidants.
Chen Yiyong, Gu Xiaohong, Huang Sheng-quan, Li Jinwei, Wang Xin, Tang Jian (2010): Optimization of ultrasonic/microwave assisted extraction (UMAE) of polysaccharides from Inonotus obliquus and evaluation of its anti-tumor activities. International Journal of Biological Macromolecules, 46, 429-435 https://doi.org/10.1016/j.ijbiomac.2010.02.003
Leonelli Cristina, Mason Timothy J. (2010): Microwave and ultrasonic processing: Now a realistic option for industry. Chemical Engineering and Processing: Process Intensification, 49, 885-900 https://doi.org/10.1016/j.cep.2010.05.006
Liu H.W., Chen H.X., Gong X.Y. (2014): Study on microwave and ultrasonic-assisted extraction of flavonoids in the sweet potapo leaves. Science and Technology of Food Industry, 16: 298–301.
Liu Q.D. (2010): Technological study on synergistic extraction of celery flavonoids by ultrasonic wave and microwave. Food and Machinery, 26: 134–137.
Sakdarat Santi, Shuyprom Aussavashai, Pientong Chamsai, Ekalaksananan Tipaya, Thongchai Sasithorn (2009): Bioactive constituents from the leaves of Clinacanthus nutans Lindau. Bioorganic & Medicinal Chemistry, 17, 1857-1860 https://doi.org/10.1016/j.bmc.2009.01.059
Liew Shan Qin, Ngoh Gek Cheng, Yusoff Rozita, Teoh Wen Hui (2016): Sequential ultrasound-microwave assisted acid extraction (UMAE) of pectin from pomelo peels. International Journal of Biological Macromolecules, 93, 426-435 https://doi.org/10.1016/j.ijbiomac.2016.08.065
Singleton V.L., Rossi J.A. (1965): Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16: 144–158.
Georgé Stephane, Brat Pierre, Alter Pascaline, Amiot Marie J. (2005): Rapid Determination of Polyphenols and Vitamin C in Plant-Derived Products. Journal of Agricultural and Food Chemistry, 53, 1370-1373 https://doi.org/10.1021/jf048396b
Tang Xunyou, Zhu Dan, Huai Wenbei, Zhang Wei, Fu Chujun, Xie Xiujuan, Quan Sisi, Fan Huajun (2017): Simultaneous extraction and separation of flavonoids and alkaloids from Crotalaria sessiliflora L. by microwave-assisted cloud-point extraction. Separation and Purification Technology, 175, 266-273 https://doi.org/10.1016/j.seppur.2016.11.038
Teshima K.I., Kaneko T., Ohtani K., Kasai R., Lhieochaiphant S., Picheansoonthon C., Yamasaki K. (1997): C-Glycosyl flavones from Clinacanthus nutans. Natural Medicines, 51: 557.
Wang F., Zhao M., Xue X.L. (2012): Study on extraction process of polysaccharide from Chrysanthemum morifolium Ramat. Northern Horticulture, 2: 28–31.
Wang H., Zhang W.M. (1995): Study on quality control of flavonoids in Ginkgo biloba leaves. Chinese Wild Plant Resources, 1: 13–16.
Wang Q.H., Liu C., Fang B. (2010): Study on extraction process of flavonoids from peanut shell. Food Science and Technology, 35: 207–210.
Wang X.E., Zhong X.W., Zhang W.X. (2013): The study on the chemical ingredients from Clinacanthus nutans and analyse its antitumor effect. China Pharmacy, 24: 4104–4107.
Yao S.J., Huang S.Q., Chen Z.Y., Zeng Q.X. (2009): Ultrasonic-assisted extraction of the triterpenoids from Ganoderma lucidum. Modern Food Science and Technology, 25: 1015–1019.
Yi B., Xu W.T., Deng D. (2012): Analysis of amino acids, trace elements and chemical constituents from the leaves of Clinacanthus nutans. Pharmaceutical Journal of Chinese People’s Liberation Army, 28: 396.
Yin Y., Ye Q., Lu Z.C. (2015): Study on ultrasonic-assisted extraction process of polysaccharide from Morinda officinalis. Northern Horticulture, 12: 124–126.
Zhang W.H., Ma Z., Sun Y. (2014): Optimization of ultrasonic-assisted extraction process for total flavonoids from Clinacanthus nutans and evaluation of its in vitro antioxidant activity. Chinese Journal of Experimental Traditional Medical Formulae, 20: 38–41.
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