Lineage and serotype identification of Listeria monocytogenes by matrix-assisted laser desorption ionization-time of flight mass spectrometry

https://doi.org/10.17221/87/2018-CJFSCitation:Koudelka Š., Gelbíčová T., Procházková M., Karpíšková R. (2018): Lineage and serotype identification of Listeria monocytogenes by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Czech J. Food Sci., 36: 452-458.
download PDF

The identification of Listeria species, lineages and serotypes remains a crucial issue not only in epidemic surveys, but also in monitoring of the diversity of bacteria in the food chain. The aim of this study was identification of L. monocytogenes strains at lineage and serotype level using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The performance of MALDI-TOF MS was tested to identify L. monocytogenes into two lineages (I and II) and four serotypes (1/2a, 1/2b, 1/2c and 4b) the most commonly found in humans and food. Total of 227 L. monocytogenes strains from different sources were subjected to the study. Some of strains (112) were used for main spectrum profile (MSP) library creation. Other strains of interest (115) were then correctly identified on the lineage level comparing with the library by MALDI-TOF MS analysis using Biotyper (90%) and ClinPro Tools (100%) software. The serotype identification with 55.7% (Biotyper) and 67.8% (ClinPro Tools) accuracy is rather a proof that under given conditions the method has not big potential to be used for serotyping. However, MALDI-TOF MS has a potential to identify lineages of L. monocytogenes of food and human origin.

References:
Angeletti Silvia (2017): Matrix assisted laser desorption time of flight mass spectrometry (MALDI-TOF MS) in clinical microbiology. Journal of Microbiological Methods, 138, 20-29  https://doi.org/10.1016/j.mimet.2016.09.003
 
Barbuddhe S. B., Maier T., Schwarz G., Kostrzewa M., Hof H., Domann E., Chakraborty T., Hain T. (2008): Rapid Identification and Typing of Listeria Species by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. Applied and Environmental Microbiology, 74, 5402-5407  https://doi.org/10.1128/AEM.02689-07
 
Cabrita Paula, Fonseca Catarina, Freitas Regina, Carreira Ricardo, Capelo Jose Luis, Trigo Maria João, Ferreira Ricardo Boavida, Brito Luisa (2010): A secretome-based methodology may provide a better characterization of the virulence of Listeria monocytogenes: Preliminary results. Talanta, 83, 457-463  https://doi.org/10.1016/j.talanta.2010.09.039
 
Doumith M., Buchrieser C., Glaser P., Jacquet C., Martin P. (2004): Differentiation of the Major Listeria monocytogenes Serovars by Multiplex PCR. Journal of Clinical Microbiology, 42, 3819-3822  https://doi.org/10.1128/JCM.42.8.3819-3822.2004
 
Dreyer M., Thomann A., Böttcher S., Frey J., Oevermann A. (2015): Outbreak investigation identifies a single Listeria monocytogenes strain in sheep with different clinical manifestations, soil and water. Veterinary Microbiology, 179, 69-75  https://doi.org/10.1016/j.vetmic.2015.01.025
 
Dumas Emilie, Meunier Bruno, Berdagué Jean-Louis, Chambon Christophe, Desvaux Mickaël, Hébraud Michel (2009): The origin of Listeria monocytogenes 4b isolates is signified by subproteomic profiling. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 1794, 1530-1536  https://doi.org/10.1016/j.bbapap.2009.06.029
 
Freiwald Anja, Sauer Sascha (2009): Phylogenetic classification and identification of bacteria by mass spectrometry. Nature Protocols, 4, 732-742  https://doi.org/10.1038/nprot.2009.37
 
Hrabak J., Studentova V., Walkova R., Zemlickova H., Jakubu V., Chudackova E., Gniadkowski M., Pfeifer Y., Perry J. D., Wilkinson K., Bergerova T. (2012): Detection of NDM-1, VIM-1, KPC, OXA-48, and OXA-162 Carbapenemases by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. Journal of Clinical Microbiology, 50, 2441-2443  https://doi.org/10.1128/JCM.01002-12
 
Hsueh P.-R., Lee T.-F., Du S.-H., Teng S.-H., Liao C.-H., Sheng W.-H., Teng L.-J. (2014): Bruker Biotyper Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry System for Identification of Nocardia, Rhodococcus, Kocuria, Gordonia, Tsukamurella, and Listeria Species. Journal of Clinical Microbiology, 52, 2371-2379  https://doi.org/10.1128/JCM.00456-14
 
Jadhav Snehal, Sevior Danielle, Bhave Mrinal, Palombo Enzo A. (2014): Detection of Listeria monocytogenes from selective enrichment broth using MALDI–TOF Mass Spectrometry. Journal of Proteomics, 97, 100-106  https://doi.org/10.1016/j.jprot.2013.09.014
 
Jadhav Snehal, Gulati Vandana, Fox Edward M., Karpe Avinash, Beale David J., Sevior Danielle, Bhave Mrinal, Palombo Enzo A. (2015): Rapid identification and source-tracking of Listeria monocytogenes using MALDI-TOF mass spectrometry. International Journal of Food Microbiology, 202, 1-9  https://doi.org/10.1016/j.ijfoodmicro.2015.01.023
 
Ketterlinus Ralf, Hsieh Sen-Yung, Teng Shih-Hua, Lee Helen, Pusch Wolfgang (2005): Fishing for biomarkers: analyzing mass spectrometry data with the new ClinProTools™ software. BioTechniques, 38, S37-S40  https://doi.org/10.2144/05386SU07
 
Nakano S., Matsumura Y., Ito Y., Fujisawa T., Chang B., Suga S., Kato K., Yunoki T., Hotta G., Noguchi T., Yamamoto M., Nagao M., Takakura S., Ohnishi M., Ihara T., Ichiyama S. (2015): Development and evaluation of MALDI-TOF MS-based serotyping for Streptococcus pneumoniae. European Journal of Clinical Microbiology & Infectious Diseases, 34, 2191-2198  https://doi.org/10.1007/s10096-015-2468-9
 
Nomura F. (2015): Proteome-based bacterial identification using matrix-assisted laser desorption ionization-time
 
of flight mass spectrometry (MALDI-TOF MS): A revolutionary shift in clinical diagnostic microbiology. Biochimica et Biophysica Acta, 1854: 528–537.
 
Ojima-Kato Teruyo, Yamamoto Naomi, Takahashi Hajime, Tamura Hiroto, Desvaux Mickaël (2016): Matrix-assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) Can Precisely Discriminate the Lineages of Listeria monocytogenes and Species of Listeria. PLOS ONE, 11, e0159730-  https://doi.org/10.1371/journal.pone.0159730
 
Orsi Renato H., Bakker Henk C. den, Wiedmann Martin (2011): Listeria monocytogenes lineages: Genomics, evolution, ecology, and phenotypic characteristics. International Journal of Medical Microbiology, 301, 79-96  https://doi.org/10.1016/j.ijmm.2010.05.002
 
Phan-Thanh Luu, Mahouin François (1999): A proteomic approach to study the acid response inListeria monocytogenes. Electrophoresis, 20, 2214-2224  https://doi.org/10.1002/(SICI)1522-2683(19990801)20:11<2214::AID-ELPS2214>3.0.CO;2-G
 
Randall Luke P., Lemma Fabrizio, Koylass Mark, Rogers Jon, Ayling Roger D., Worth Danny, Klita Monika, Steventon Andrew, Line Kirsty, Wragg Peter, Muchowski Jakub, Kostrzewa Markus, Whatmore Adrian M. (2015): Evaluation of MALDI-ToF as a method for the identification of bacteria in the veterinary diagnostic laboratory. Research in Veterinary Science, 101, 42-49  https://doi.org/10.1016/j.rvsc.2015.05.018
 
Singhal Neelja, Kumar Manish, Kanaujia Pawan K., Virdi Jugsharan S. (2015): MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis. Frontiers in Microbiology, 6, -  https://doi.org/10.3389/fmicb.2015.00791
 
Stevenson L. G., Drake S. K., Murray P. R. (2010): Rapid Identification of Bacteria in Positive Blood Culture Broths by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. Journal of Clinical Microbiology, 48, 444-447  https://doi.org/10.1128/JCM.01541-09
 
Trémoulet F (): Comparison of protein patterns of Listeria monocytogenes grown in biofilm or in planktonic mode by proteomic analysis. FEMS Microbiology Letters, 210, 25-31  https://doi.org/10.1016/S0378-1097(02)00571-2
 
Tsai Y.H., Maron S.B., McGann P., Nightingale K.K., Wiedmann M., Orsi R.H. (2011): Recombination and positive selection contributed to the evolution of Listeria monocytogenes lineages III and IV, two distinct and well supported uncommon
 
L. monocytogenes lineages. Infections, Genetics and Evolution, 11: 1881–1890.
 
Wieser Andreas, Schneider Lukas, Jung Jette, Schubert Sören (2012): MALDI-TOF MS in microbiological diagnostics—identification of microorganisms and beyond (mini review). Applied Microbiology and Biotechnology, 93, 965-974  https://doi.org/10.1007/s00253-011-3783-4
 
download PDF

© 2019 Czech Academy of Agricultural Sciences