Unusual behavior of natural polyphosphates during IMAC
M. Kmínková, A. Prošková, J. Kučerahttps://doi.org/10.17221/6601-CJFSCitation:Kmínková M., Prošková A., Kučera J. (2001): Unusual behavior of natural polyphosphates during IMAC. Czech J. Food Sci., 19: 161-165.
The atypical course of IDA-ferric (Imino-Di-Acetyl-agarose saturated with ferric ions) of two polyphosphorylated compounds – phytic acid and Ser(P)-Ser(P)-Ser(P)-Glu-Glu cluster obtained by the way of pancreatic hydrolysis of bovine casein was detected. The spectral data (for phytic acid) and iron content (for polyphosphate cluster) indicate the strong bond of ferric ions to these polyphosphorylated compounds. The data shows these compounds to bind ferric ion stronger even in comparison with IDA (Imino-Di-Acetate) itself. Therefore attention is to be paid to all IMAC (Immobilized-Metal-Ion-Affinity-Chromatography) of natural compounds containing strong chelating groups or several chelating groups in vicinity.Keywords:
phytic acid; Ser(P)-Ser(P)-Ser(P)-Glu-Glu cluster; affinity chromatography; immobilized metal ion; IMAC; IDA-ferric; atypical adsorption