Beta-galactosidase activity in psychrotrophic microorganisms and their potential use in food industry
P. Karasová, V. Spiwok, Š. Malá, B. Králová, N.J. Russellhttps://doi.org/10.17221/3508-CJFSCitation:Karasová P., Spiwok V., Malá Š., Králová B., Russell N.J. (2002): Beta-galactosidase activity in psychrotrophic microorganisms and their potential use in food industry. Czech J. Food Sci., 20: 43-47.
Twenty-one psychrotrophic resp. psychrophilic bacterial strains were screened for presence of b-galactosidase activity which showed 8 of them. b-Galactosidase activity of these strains was determined for 2 substrates – synthetic substrate (ONPG) and lactose – and also temperature profile of this enzyme was measured. b-Galactosidase from Arthrobacter sp. C2-2 not only proved the typical properties of cold-active enzyme, but it also preferred lactose as a substrate. Therefore, it was chosen for further isolation and purification and was found that it contains two b-galactosidase isoenzymes. One of them had strong preference for lactose and was able to catalyse transglycosylation reactions at low temperature. It has, thus, potential use in food technology.Keywords:
psychrotrophic microorganism; cold-active enzyme; b-galactosidase; lactose hydrolysis; transglycosylation