13C CP/MAS NMR spectra of pectins: a peak-fitting analysis in the C-6 region
A. Synytsya, J. opíková, J. Brushttps://doi.org/10.17221/3471-CJFSCitation:Synytsya A., opíková J., Brus J. (2003): 13C CP/MAS NMR spectra of pectins: a peak-fitting analysis in the C-6 region. Czech J. Food Sci., 21: 1-12.
13C CP/MAS NMR spectra of pectin samples were measured and interpreted. The influence of methylesterification and O-acetylation on chemical shifts of pectin carbons was studied using model pectate, pectinates, and their acetylated derivatives. The spectra were analysed also by peak fitting in C-6 region to obtain the values of methylation (DM), amidation (DAm) and acetylation (DAc), degrees. The results were in a good agreement with both convenient methods (photometry, elemental analysis) and the NMR method based on the relative areas of OCH3 (DM) and CH3 (DAc) resonances.Keywords:
13C CP/MAS NMR; pectins; peak fitting; degrees of methylation (DM); degrees of acetylation (DAc)