Determination of Lactoferrin in Goat Milk by HPLC Method
M. Dračková, I. Borkovcová, B. Janštová, M. Naiserová, H. Přidalová, P. Navrátilová, L. Vorlováhttps://doi.org/10.17221/944-CJFSCitation:Dračková M., Borkovcová I., Janštová B., Naiserová M., Přidalová H., Navrátilová P., Vorlová L. (2009): Determination of Lactoferrin in Goat Milk by HPLC Method. Czech J. Food Sci., 27: S102-S104.
The aim of this study was the determination of lactoferrin in goat milk using HPLC method. Milk samples were collected at a goat farm in the South Moravia Region, the Czech Republic. It were established bulk tank samples of raw milk (n = 24) and pasteurised milk (nn = 27) that were collected during lactation. Lactoferrin contents were analysed by reverse phase high-performance liquid chromatography (RP-HPLC) with diode-array detector PDA 2996. Detection was carried out at the wavelength 205 nm. The average concentration of lactoferrin in goat milk was 120 ± 18 μg/ml. The lactoferrin content was increasing within the lactation period in the ranges of 98 ± 170 μg/ml in April to 149 ± 19 μg/ml in November. The heat treatment (pasteurisation at 72°C for 20 s) resulted in no significant effect on the lactoferrin content. No statistically significant differences (P = 0.05) were found between the values of raw and pasteurised goat milk.Keywords:lactoferrin; milk; RP HPLC; goat