Anticancer Activity of Lignan from the Aerial Parts of Saussurea salicifolia (L.) DC.
G. Chunsriimyatav, I. Hoza, P. Valášek, S. Skrovanková, D. Banzragch, N. Tsevegsurenhttps://doi.org/10.17221/1097-CJFSCitation:Chunsriimyatav G., Hoza I., Valášek P., Skrovanková S., Banzragch D., Tsevegsuren N. (2009): Anticancer Activity of Lignan from the Aerial Parts of Saussurea salicifolia (L.) DC. Czech J. Food Sci., 27: S256-S258.
Aerial parts of Saussurea salicifolia (L.) DC were studied for their lignan and flavonoids in solvent chloroform and n-butanol of ethanolic extract. Isolation and identification of phenolic compounds of the chloroform and n-butanol fractions were performed with Dionex HPLC-DAD system with water-methanol gradients in 4 different wave lengths (235 nm, 254 nm, 280 nm and 340 nm), using online UV and LC-MS as described previously. 9-OH-pinoresinol which is a lignan with anticancer activity was dominated in the chloroform fraction, whereas mainly flavonoid glycosides like quercetin-3-O-galactoside, apigenin-7-O-rhamnoside with anti-inflammatory effect were detected in the n-butanol fraction. Additionally, 9-OH-pinoresinol was also found in the n-butanol fraction. Anticancer tests were conducted in leukemia mouse lymphoma cells L5178Y at a concentration of 10 μg/ml of test compound. Crude ethanol extract of S. salicifolia reduced the growth of leukemia mouse lymphoma cells L5178Y to 23.8%.Keywords:flavonoids; Saussurea salicifolia; anticancer activity; Dionex HPLC-DAD system