Mineral profile of Croatian honey and differences due to its geographical origin

https://doi.org/10.17221/502/2014-CJFSCitation:Uršulin-Trstenjak N., Levanić D., Primorac L., Bošnir J., Vahčić N., Šarić G. (2015): Mineral profile of Croatian honey and differences due to its geographical origin. Czech J. Food Sci., 33: 156-164.
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The proportions of twelve minerals were determined in 200 samples of the black locust honey from five Croatian regions during two seasons. The average proportions were dominated by that of K (205.57–428.05 mg/kg), followed by Ca (33.53–329.00 mg/kg) and Na (23.34–218.04mg/kg), which was in percentages as follows: K 31.69–81.34%, Ca 6.51–35.56%, and Na 7.36–23.65%. Melissopalynological and physico-chemical analysis of honey confirmed its general quality requirements and botanical origin. One-factor analysis of variance showed a significant differences between the regions in each season based on the average proportions of macro and micro elements except for Mn and Cd in season 2 (P < 0.05). t-Test enabled an insight into the significance of differences between the seasons within each region based on the average proportions of macro and micro elements. Principal components analysis (PCA) showed that Al (Bjelovar-Bilogora), Fe (Bjelovar-Bilogora and Istria), Cu (Eastern Croatia), and K (Istria) could present mineral substances typical for the black locust honey of each region.
References:
Anklam Elke (1998): A review of the analytical methods to determine the geographical and botanical origin of honey. Food Chemistry, 63, 549-562  https://doi.org/10.1016/S0308-8146(98)00057-0
 
Azeredo L.da C, Azeredo M.A.A, de Souza S.R, Dutra V.M.L (2003): Protein contents and physicochemical properties in honey samples of Apis mellifera of different floral origins. Food Chemistry, 80, 249-254  https://doi.org/10.1016/S0308-8146(02)00261-3
 
AJTONY Z, BENCS L, HARASZI R, SZIGETI J, SZOBOSZLAI N (2007): Study on the simultaneous determination of some essential and toxic trace elements in honey by multi-element graphite furnace atomic absorption spectrometry. Talanta, 71, 683-690  https://doi.org/10.1016/j.talanta.2006.05.023
 
Belouali H., Bouaka M., Hakkou A. (2008): Determination of some major and minor elements in the east of Morocco honeys through inductively coupled plasma optical emission spectrometry. Apiacta, 43: 17–24.
 
Bogdanov S. (2009): The Book of Honey. Bee Product Science. 46: 269–275. Available at www.bee-hexagon.net
 
Bogdanov Stefan, Ruoff Kaspar, Persano Oddo Livia (2004): Physico-chemical methods for the characterisation of unifloral honeys: a review. Apidologie, 35, S4-S17  https://doi.org/10.1051/apido:2004047
 
Bogdanov S., Haldimann M., Luginbühl W., Gallmann P. (2007): Minerals in honey: environmental, geographical and botanical aspects. Journal of Apicultural Research and Bee World, 46: 269–275
 
Bubalo D., Dražić M., Mandić M.L., Kenjerić D., Kezić N. (2006): Botaničko podrijetlo meda s područja jadranske regije. In: Jovanovac S., Kovačević V. (eds): 41. Hrvatski i 1. Međunarodni Znanstveni simpozij Agronoma, Poljoprivredni Fakultet, Osijek, Oct 26, 2006: 549–550.
 
Caroli S., Forte G., Alessandrelli M., Cresti R., Spagnoli M., D'Ilio S., Pauwels J., Kramer G.N. (2000): A pilot study for the production of a certified reference material for trace elements in honey. Microchemical Journal, 67, 227-233  https://doi.org/10.1016/S0026-265X(00)00066-7
 
Conti Marcelo, Stripeikis Jorge, Campanella Luigi, Cucina Domenico, Tudino Mabel (): Characterization of Italian honeys (Marche Region) on the basis of their mineral content and some typical quality parameters. Chemistry Central Journal, 1, 14-  https://doi.org/10.1186/1752-153X-1-14
 
DIN 10750:1990: Bestimmung der Diastase-Aktivität. Deutsches Institut für Normung.
 
DIN 10760:2002-05: Determination of relative pollen content of honey. Deutsches Institut für Normung.
 
Čelechovská O., Vorlová L. (2001): Groups of honey – physicochemical properties and heavy metals. Acta Vete-
 
rinaria, 70: 91–95.
 
ELAN ICP-MS (2008): Series Manuals. Waltham, Perkin Elmer.
 
European Commission – Council Directive 2001/110/EC (2002): Official Journal of the European Communities, L10: 47–52.
 
FERNANDEZTORRES R, PEREZBERNAL J, BELLOLOPEZ M, CALLEJONMOCHON M, JIMENEZSANCHEZ J, GUIRAUMPEREZ A (2005): Mineral content and botanical origin of Spanish honeys. Talanta, 65, 686-691  https://doi.org/10.1016/j.talanta.2004.07.030
 
Forte G., D’Ilio S., Caroli S. (2001): Honey as a candidate reference material for trace elements. Journal of AOAC International, 84: 1972–1975.
 
Golob T., Doberšek U., Kump P., Nečemer M. (2005): Determination of trace and minor elements in Slovenian honey by total reflection X-ray fluorescence spectroscopy. Food Chemistry, 91, 593-600  https://doi.org/10.1016/j.foodchem.2004.04.043
 
Harmonised Methods of the International (European) Honey Commission (2009): Available at http://www.bee-hexagon.net/files/file/fileE/IHC-methods_2009.pdf
 
HRN EN ISO 17294-1 (2008): Kakvoća vode –Primjena masene spektrometrije s induktivno spregnutom plazmom (ICP-MS) za određivanje elemenata – 1. dio: Opće smjernice i osnovna načela + 2. dio: Određivanje 62 elementa. Hrvatski zavod za norme
 
HRN EN 15763 (2010): Namirnice – Određivanje elemenata u tragovima – Određivanje arsena, kadmija, žive i olova u hrani induktivno spregnutom plazmom sa spektrometrijom masa (ICP-MS) nakon razgradnje pod tlakom. Hrvatski zavod za norme
 
Iglesias María Teresa, de Lorenzo Cristina, Polo María del Carmen, Martín-Álvarez Pedro Jésus, Pueyo Encarnacíon (2004): Usefulness of Amino Acid Composition To Discriminate between Honeydew and Floral Honeys. Application to Honeys from a Small Geographic Area. Journal of Agricultural and Food Chemistry, 52, 84-89  https://doi.org/10.1021/jf030454q
 
KENJERIC D, MANDIC M, PRIMORAC L, BUBALO D, PERL A (2007): Flavonoid profile of Robinia honeys produced in Croatia. Food Chemistry, 102, 683-690  https://doi.org/10.1016/j.foodchem.2006.05.055
 
Kolayli S., Kongur N., Gundogdu A., Kemer B., Duran Aliyazicioglu C. (2008): Mineral composition of selected honeys from Turkey. Asian Journal of Chemistry, 20: 2421–2425.
 
Latorre M (): Chemometric classification of honeys according to their type. II. Metal content data. Food Chemistry, 66, 263-268  https://doi.org/10.1016/S0308-8146(98)00217-9
 
Madejczyk Maria, Baralkiewicz Danuta (2008): Characterization of Polish rape and honeydew honey according to their mineral contents using ICP-MS and F-AAS/AES. Analytica Chimica Acta, 617, 11-17  https://doi.org/10.1016/j.aca.2008.01.038
 
Marghitas L.A., Dezmirean D.S., Pocol C.B., Ilea M., Bobis O., Gergen I. (2010): The development of a biochemical profile of acacia honey by identifying biochemical determinants of its quality. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38 (Special Issue): 84–90.
 
Mateo Rufino, Bosch-Reig Francisco (1997): Sugar profiles of Spanish unifloral honeys. Food Chemistry, 60, 33-41  https://doi.org/10.1016/S0308-8146(96)00297-X
 
MPRRR RH (2009a): Pravilnik o medu. Narodne novine 93/09.
 
MPRRR RH (2009b): Pravilnik o kakvoći uniflornog meda. Narodne novine 122/09.
 
Muñoz E., Palmero S. (2005): Determination of heavy metals in honey by potentiometric stripping analysis and using a continuous flow methodology. Food Chemistry, 52: 313–319.
 
Nanda Vikas, Sarkar B.C., Sharma H.K., Bawa A.S. (2003): Physico-chemical properties and estimation of mineral content in honey produced from different plants in Northern India. Journal of Food Composition and Analysis, 16, 613-619  https://doi.org/10.1016/S0889-1575(03)00062-0
 
Osman K.A., Al-Doghairi M.A., Al-Rehiayani S., Helal M.I.D. (2007): Mineral contents and physicochemical properties of natural honey produced in Al-Qassim region, Saudi Arabia. Journal of Food & Environment, 5: 142–146.
 
Perkin Elmer Sciex: 30-minute Guide to ICP/MS (2004).
 
Persano Oddo Livia, Bogdanov Stefan (2004): Determination of honey botanical origin: problemsand issues. Apidologie, 35, S2-S3  https://doi.org/10.1051/apido:2004044
 
Persano Oddo Livia, Piana Lucia, Bogdanov Stefan, Bentabol Antonio, Gotsiou Panagiota, Kerkvliet Jacob, Martin Peter, Morlot Monique, Ortiz Valbuena Alberto, Ruoff Kaspar, von der Ohe Katharine (2004): Botanical species giving unifloral honey in Europe. Apidologie, 35, S82-S93  https://doi.org/10.1051/apido:2004045
 
Přidal A., Flanjak I., Vorlová L. (2002): Honey and its physical parameters. Czech Journal of Food Sciences, 47: 439–444.
 
Primorac L., Flanjak I., Kenjerić D., Bubalo D., Topolnjak Z. (2011): Specific rotation and carbohydrate profile of Croatian unifloral honeys. Czech Journal of Food Sciences, 29: 515–519.
 
Przybyowski Piotr, Wilczyńska Aleksandra (2001): Honey as an environmental marker. Food Chemistry, 74, 289-291  https://doi.org/10.1016/S0308-8146(01)00153-4
 
Schweizerisches Lebensmittelbuch (1995): Bestimmung der Amylactivität (nach Phadebas). SLMB Kapitel 23A Honig. Bern, EDMZ.
 
Silva Luís R., Videira Romeu, Monteiro Andreia P., Valentão Patrícia, Andrade Paula B. (2009): Honey from Luso region (Portugal): Physicochemical characteristics and mineral contents. Microchemical Journal, 93, 73-77  https://doi.org/10.1016/j.microc.2009.05.005
 
Šarić G., Matković D., Hruškar M., Vahčić N. (2008): Characterisation and classification of croatian honey by physicochemical parameters. Food Technology and Biotechnology, 46: 355–367.
 
Thomas R. (2008): Practical Guide to ICP-MS: A Tutorial for Beginners. 2nd Ed. New York, CRC Press.
 
Uršulin-Trstenjak N., Hrga I., Stjepanović B., Dragojlović D., Levanić D. (2013): Determination of botanic origin of the Croatian black locust honey (Istria region) using melissopalynological analysis. Journal of Hygienic Engineering and Design, 4: 122–126.
 
Anklam Elke (1998): A review of the analytical methods to determine the geographical and botanical origin of honey. Food Chemistry, 63, 549-562  https://doi.org/10.1016/S0308-8146(98)00057-0
 
Azeredo L.da C, Azeredo M.A.A, de Souza S.R, Dutra V.M.L (2003): Protein contents and physicochemical properties in honey samples of Apis mellifera of different floral origins. Food Chemistry, 80, 249-254  https://doi.org/10.1016/S0308-8146(02)00261-3
 
AJTONY Z, BENCS L, HARASZI R, SZIGETI J, SZOBOSZLAI N (2007): Study on the simultaneous determination of some essential and toxic trace elements in honey by multi-element graphite furnace atomic absorption spectrometry. Talanta, 71, 683-690  https://doi.org/10.1016/j.talanta.2006.05.023
 
Belouali H., Bouaka M., Hakkou A. (2008): Determination of some major and minor elements in the east of Morocco honeys through inductively coupled plasma optical emission spectrometry. Apiacta, 43: 17–24.
 
Bogdanov S. (2009): The Book of Honey. Bee Product Science. 46: 269–275. Available at www.bee-hexagon.net
 
Bogdanov Stefan, Ruoff Kaspar, Persano Oddo Livia (2004): Physico-chemical methods for the characterisation of unifloral honeys: a review. Apidologie, 35, S4-S17  https://doi.org/10.1051/apido:2004047
 
Bogdanov S., Haldimann M., Luginbühl W., Gallmann P. (2007): Minerals in honey: environmental, geographical and botanical aspects. Journal of Apicultural Research and Bee World, 46: 269–275
 
Bubalo D., Dražić M., Mandić M.L., Kenjerić D., Kezić N. (2006): Botaničko podrijetlo meda s područja jadranske regije. In: Jovanovac S., Kovačević V. (eds): 41. Hrvatski i 1. Međunarodni Znanstveni simpozij Agronoma, Poljoprivredni Fakultet, Osijek, Oct 26, 2006: 549–550.
 
Caroli S., Forte G., Alessandrelli M., Cresti R., Spagnoli M., D'Ilio S., Pauwels J., Kramer G.N. (2000): A pilot study for the production of a certified reference material for trace elements in honey. Microchemical Journal, 67, 227-233  https://doi.org/10.1016/S0026-265X(00)00066-7
 
Conti Marcelo, Stripeikis Jorge, Campanella Luigi, Cucina Domenico, Tudino Mabel (): Characterization of Italian honeys (Marche Region) on the basis of their mineral content and some typical quality parameters. Chemistry Central Journal, 1, 14-  https://doi.org/10.1186/1752-153X-1-14
 
DIN 10750:1990: Bestimmung der Diastase-Aktivität. Deutsches Institut für Normung.
 
DIN 10760:2002-05: Determination of relative pollen content of honey. Deutsches Institut für Normung.
 
Čelechovská O., Vorlová L. (2001): Groups of honey – physicochemical properties and heavy metals. Acta Vete-
 
rinaria, 70: 91–95.
 
ELAN ICP-MS (2008): Series Manuals. Waltham, Perkin Elmer.
 
European Commission – Council Directive 2001/110/EC (2002): Official Journal of the European Communities, L10: 47–52.
 
FERNANDEZTORRES R, PEREZBERNAL J, BELLOLOPEZ M, CALLEJONMOCHON M, JIMENEZSANCHEZ J, GUIRAUMPEREZ A (2005): Mineral content and botanical origin of Spanish honeys. Talanta, 65, 686-691  https://doi.org/10.1016/j.talanta.2004.07.030
 
Forte G., D’Ilio S., Caroli S. (2001): Honey as a candidate reference material for trace elements. Journal of AOAC International, 84: 1972–1975.
 
Golob T., Doberšek U., Kump P., Nečemer M. (2005): Determination of trace and minor elements in Slovenian honey by total reflection X-ray fluorescence spectroscopy. Food Chemistry, 91, 593-600  https://doi.org/10.1016/j.foodchem.2004.04.043
 
Harmonised Methods of the International (European) Honey Commission (2009): Available at http://www.bee-hexagon.net/files/file/fileE/IHC-methods_2009.pdf
 
HRN EN ISO 17294-1 (2008): Kakvoća vode –Primjena masene spektrometrije s induktivno spregnutom plazmom (ICP-MS) za određivanje elemenata – 1. dio: Opće smjernice i osnovna načela + 2. dio: Određivanje 62 elementa. Hrvatski zavod za norme
 
HRN EN 15763 (2010): Namirnice – Određivanje elemenata u tragovima – Određivanje arsena, kadmija, žive i olova u hrani induktivno spregnutom plazmom sa spektrometrijom masa (ICP-MS) nakon razgradnje pod tlakom. Hrvatski zavod za norme
 
Iglesias María Teresa, de Lorenzo Cristina, Polo María del Carmen, Martín-Álvarez Pedro Jésus, Pueyo Encarnacíon (2004): Usefulness of Amino Acid Composition To Discriminate between Honeydew and Floral Honeys. Application to Honeys from a Small Geographic Area. Journal of Agricultural and Food Chemistry, 52, 84-89  https://doi.org/10.1021/jf030454q
 
KENJERIC D, MANDIC M, PRIMORAC L, BUBALO D, PERL A (2007): Flavonoid profile of Robinia honeys produced in Croatia. Food Chemistry, 102, 683-690  https://doi.org/10.1016/j.foodchem.2006.05.055
 
Kolayli S., Kongur N., Gundogdu A., Kemer B., Duran Aliyazicioglu C. (2008): Mineral composition of selected honeys from Turkey. Asian Journal of Chemistry, 20: 2421–2425.
 
Latorre M (): Chemometric classification of honeys according to their type. II. Metal content data. Food Chemistry, 66, 263-268  https://doi.org/10.1016/S0308-8146(98)00217-9
 
Madejczyk Maria, Baralkiewicz Danuta (2008): Characterization of Polish rape and honeydew honey according to their mineral contents using ICP-MS and F-AAS/AES. Analytica Chimica Acta, 617, 11-17  https://doi.org/10.1016/j.aca.2008.01.038
 
Marghitas L.A., Dezmirean D.S., Pocol C.B., Ilea M., Bobis O., Gergen I. (2010): The development of a biochemical profile of acacia honey by identifying biochemical determinants of its quality. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38 (Special Issue): 84–90.
 
Mateo Rufino, Bosch-Reig Francisco (1997): Sugar profiles of Spanish unifloral honeys. Food Chemistry, 60, 33-41  https://doi.org/10.1016/S0308-8146(96)00297-X
 
MPRRR RH (2009a): Pravilnik o medu. Narodne novine 93/09.
 
MPRRR RH (2009b): Pravilnik o kakvoći uniflornog meda. Narodne novine 122/09.
 
Muñoz E., Palmero S. (2005): Determination of heavy metals in honey by potentiometric stripping analysis and using a continuous flow methodology. Food Chemistry, 52: 313–319.
 
Nanda Vikas, Sarkar B.C., Sharma H.K., Bawa A.S. (2003): Physico-chemical properties and estimation of mineral content in honey produced from different plants in Northern India. Journal of Food Composition and Analysis, 16, 613-619  https://doi.org/10.1016/S0889-1575(03)00062-0
 
Osman K.A., Al-Doghairi M.A., Al-Rehiayani S., Helal M.I.D. (2007): Mineral contents and physicochemical properties of natural honey produced in Al-Qassim region, Saudi Arabia. Journal of Food & Environment, 5: 142–146.
 
Perkin Elmer Sciex: 30-minute Guide to ICP/MS (2004).
 
Persano Oddo Livia, Bogdanov Stefan (2004): Determination of honey botanical origin: problemsand issues. Apidologie, 35, S2-S3  https://doi.org/10.1051/apido:2004044
 
Persano Oddo Livia, Piana Lucia, Bogdanov Stefan, Bentabol Antonio, Gotsiou Panagiota, Kerkvliet Jacob, Martin Peter, Morlot Monique, Ortiz Valbuena Alberto, Ruoff Kaspar, von der Ohe Katharine (2004): Botanical species giving unifloral honey in Europe. Apidologie, 35, S82-S93  https://doi.org/10.1051/apido:2004045
 
Přidal A., Flanjak I., Vorlová L. (2002): Honey and its physical parameters. Czech Journal of Food Sciences, 47: 439–444.
 
Primorac L., Flanjak I., Kenjerić D., Bubalo D., Topolnjak Z. (2011): Specific rotation and carbohydrate profile of Croatian unifloral honeys. Czech Journal of Food Sciences, 29: 515–519.
 
Przybyowski Piotr, Wilczyńska Aleksandra (2001): Honey as an environmental marker. Food Chemistry, 74, 289-291  https://doi.org/10.1016/S0308-8146(01)00153-4
 
Schweizerisches Lebensmittelbuch (1995): Bestimmung der Amylactivität (nach Phadebas). SLMB Kapitel 23A Honig. Bern, EDMZ.
 
Silva Luís R., Videira Romeu, Monteiro Andreia P., Valentão Patrícia, Andrade Paula B. (2009): Honey from Luso region (Portugal): Physicochemical characteristics and mineral contents. Microchemical Journal, 93, 73-77  https://doi.org/10.1016/j.microc.2009.05.005
 
Šarić G., Matković D., Hruškar M., Vahčić N. (2008): Characterisation and classification of croatian honey by physicochemical parameters. Food Technology and Biotechnology, 46: 355–367.
 
Thomas R. (2008): Practical Guide to ICP-MS: A Tutorial for Beginners. 2nd Ed. New York, CRC Press.
 
Uršulin-Trstenjak N., Hrga I., Stjepanović B., Dragojlović D., Levanić D. (2013): Determination of botanic origin of the Croatian black locust honey (Istria region) using melissopalynological analysis. Journal of Hygienic Engineering and Design, 4: 122–126.
 
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