Study of informative DNA markers of the Rf1 gene in sunflower for breeding practice N., Usatov A., Makarenko M., Azarin K., Gorbachenko O., Kolokolova N., Usatenko T., Markina O., Gavrilova V. (2017): Study of informative DNA markers of the Rf1 gene in sunflower for breeding practice. Czech J. Genet. Plant Breed., 53: 69-75.
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The investigation of DNA markers associated with the pollen fertility restoration gene (Rf1) was conducted in Helianthus. Two sequence-characterized amplified region (SCAR) markers – HRG01 and HRG02 were informative for the identification of Rf1 gene in selections of sunflower plants. The codominant character of the HRG01 marker and HRG01 amplicon polymorphism has been determined. Five annual and twenty-six perennial species of sunflower were tested for the presence of Rf genotypes. HRG02 proved to be a more appropriate marker for Rf1 determination in perennial species, and HRG01 was more informative for annual species. We have also developed the multiplex RT-PCR test system, which allows simultaneously detecting the dominant allele of Rf1 and CMS-PET1 associated mitotype.  

Dong D.K, Li Z., Yuan F.J., Zhu S.L., Chen P., Yu W., Yang Q.H., Fu X.J., Yu X.M., Li B.Q., Zhu D.H. (2012): Inheritance and fine mapping of a restorer-of-fertility (Rf) gene for the cytoplasmic male sterility in soybean. Plant Science, 188–189: 36–40.
Galeano Carlos H, Cortés Andrés J, Fernández Andrea C, Soler Álvaro, Franco-Herrera Natalia, Makunde Godwill, Vanderleyden Jos, Blair Matthew W (2012): Gene-Based Single Nucleotide Polymorphism Markers for Genetic and Association Mapping in Common Bean. BMC Genetics, 13, 48-
Hall T.A. (1999): BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium, 41: 95–98.
Horn R. (2006): Recombination: cytoplasmic male sterility and fertility restoration in higher plants. Progress in Botany, 67: 31–52.
Horn Renate, K�hler Rainer Hans, Zetsche Klaus (1991): A mitochondrial 16 kDa protein is associated with cytoplasmic male sterility in sunflower. Plant Molecular Biology, 17, 29-36
Horn R., Kusterer B., Lazarescu E., Prüfe M., Friedt W. (2003): Molecular mapping of the Rf1 gene restoring pollen fertility in PET1-based F1 hybrids in sunflower (Helianthus annuus L.). Theoretical and Applied Genetics, 106, 599-606
Markin N. V., Usatov A. V., Vasilenko V. N., Klimenko A. I., Gorbachenko O. F., Maidanyuk D. N., Getmantseva L. V. (2016): SSR Analysis of Maternal and Paternal Lines Selected in the Don Region (Russia). American Journal of Agricultural and Biological Sciences, 11, 13-18
Pratap A., Kumar J. (2014): Alien Gene Transfer in Crop Plants. Volume 2: Achievements and Impacts. New York, Springer Science Business Media: 281–315.
Stanley R.G., Linskens H.F. (1974): Pollen: Biology, Biochemistry, and Management. New York, Springer Verlag.
Tang S., Yu J.-K., Slabaugh M.B., Shintani D.K., Knapp S.J. (2002): Simple sequence repeat map of the sunflower genome. Theoretical and Applied Genetics, 105, 1124-1136
Ui Hajime, Sameri Mohammad, Pourkheirandish Mohammad, Chang Men-Chi, Shimada Hiroaki, Stein Nils, Komatsuda Takao, Handa Hirokazu (2015): High-resolution genetic mapping and physical map construction for the fertility restorer Rfm1 locus in barley. Theoretical and Applied Genetics, 128, 283-290
Yue B., Vick B. A., Cai X., Hu J. (2010): Genetic mapping for the Rf1 (fertility restoration) gene in sunflower ( Helianthus annuus L.) by SSR and TRAP markers. Plant Breeding, 129, 24-28
Zhang Z. F., Wang Y., Zheng Y. L. (2006): AFLP and PCR-based markers linked to Rf3, a fertility restorer gene for S cytoplasmic male sterility in maize. Molecular Genetics and Genomics, 276, 162-169
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