Evaluation of genetic variability in Algerian clover (Trifolium L.) based on morphological and isozyme markers

https://doi.org/10.17221/10/2015-CJGPBCitation:Medoukali I., Bellil I., Khelifi D. (2015): Evaluation of genetic variability in Algerian clover (Trifolium L.) based on morphological and isozyme markers. Czech J. Genet. Plant Breed., 51: 50-61.
download PDF
Genetic variation within and among fifteen Trifolium species represented by 157 accessions was assessed using morphological and isozyme markers. Most of morphometric characters contributed to the discrimination of the species. No significant relationship between the environment of the collection site and morphological features was detected. The two isozyme systems analysed, esterase (EST) and glutamate oxaloacetate transaminase (GOT), proved polymorphic. Phenotype diversity of isozyme markers ranged from 0.07 to 0.61 with an average of 0.31 based on the polymorphic information content. The pairwise Jaccard’s similarity coefficient ranged between 0.10 and 0.60, indicating that the collection represents genetically diverse species. A considerable number of species-specific zymograms were detected which can be used for the species identification. The clustering pattern of isozyme markers was incongruent with the groupings based on quantitative traits. The rich isozyme variability present among the Algerian clover species indicates that they can provide good gene resources for breeding.
Abberton M. T. (2007): Interspecific hybridization in the genus Trifolium. Plant Breeding, 126, 337-342  https://doi.org/10.1111/j.1439-0523.2007.01374.x
Addinsoft (2007): XLSTAT, Analyse de données et statistique avec MS Excel. New York, Addinsoft.
Allen O.N., Allen E.K. (1981): The Leguminosae. A Source Book of Characteristics, Uses and Nodulation. Madison, University of Wisconsin Press.
Asci O.O. (2011): Biodiversity in red clover (Trifolium pratense L.) collected from Turkey. I: Morpho-agronomic properties. African Journal of Biotechnology, 10: 14073–14079.
Bendiab K., Baaziz M., Brakez Z., Sedra My.H. (1993): Correlation of isoenzyme polymorphism and Bayoud-disease resistance in date palm cultivars and progeny. Euphytica, 65: 23–32.
Dias P.M.B., Julier B., Sampoux J.P., Barre P., Dall’Agnol M. (2008a): Genetic diversity in red clover (Trifolium pretense L.) revealed by morphological and microsatellite (SSR) markers. Euphytica, 160: 189–205.
Dias P.M.B., Pretz V.F., Dall’Agnol M., Schifino-Wittmann M.T., Zuanazzi J.A. (2008b): Analysis of genetic diversity in the core collection of red clover (Trifolium pratense) with isozyme and RAPD markers. Crop Breeding and Applied Biotechnology, 8: 202–211.
Drobná J. (2009): Yield and forage quality of Romanian red clover (Trifolium pratense L.) varieties studied in Slovakia. Notulae Botanicae Horti Agrobotanici, 37: 204–208.
Ellison N.W., Liston A., Steiner J.J., Williams W.M., Taylor N.L. (2006): Molecular phylogenetics of the clover genus (Trifolium – Leguminosae). Molecular Phylogenetics and Evolution, 39: 688–705.
Ghoubay A., Abdelguerfi A. (1991): Spontaneous species of the genus Trifolium L. in Algeria: Comparison of seeds and inflorescences in thirteen clovers. In: Proc. 4th Int. Rangeland Congr., Montpellier, Apr 22–26, 1991: 375–376.
Greene S.L., Gritsenko M., Vandemark G. (2004): Relating morphologic and RAPD marker variation to collection site environment in wild populations of red clover (Trifolium pratense L.). Genetic Resources and Crop Environment, 51: 643–653.
Issolah R. (1997): Comportement, biométrie et caryologie de populations spontanées de treize espèces du genre Trifolium L. en Algérie. [MSc. Thesis.] El Harrach, INA.
Issolah R., Abdelguerfi A. (1999a): Chromosome numbers within some spontaneous populations of Trifolium species in Algeria. Caryologia, 52: 151–154.
Issolah R., Abdelguerfi A. (1999b): Variability within 31 spontaneous populations of Trifolium scabrum L., nature of relations with factors of the site of origin. In: 9th Meeting of the FAO-CIHEAM Sub-Network on Mediterranean Pastures and Fodder Crops, Badajoz, Nov 26–29, 1997: 123–127.
Issolah R., Abdelguerfi A. (2000): Study of the variability in 28 spontaneous populations of Trifolium tomentosum L.: Relations with factors of the environment of origin. In: Proc. 10th Meeting of the Mediterranean Subnetwork of the FAO-CIHEAM Inter-Regional on Pasture and Fodder Crops, Sassari, Apr 4–9, 2000: 29–32.
Issolah R., Abdelguerfi A. (2003): Morphological variability within four Algerian populations of the species Trifolium spumosum L. In: 25th EUCARPIA Fodder Crops and Amenity Grasses Section Meeting, Brno, Sept 1–4, 2003: 205–208.
Jaccard P. (1908): Nouvelles recherches sur la distribution florale. Bulletin de la Société Vaudoise des Sciences Naturelles, 44: 223–270.
Kongkiatngam P., Waterway M. J., Fortin M. G., Coulman B. E. (1995): Genetic variation within and between two cultivars of red clover (Trifolium pratense L.): Comparisons of morphological, isozyme, and RAPD markers. Euphytica, 84, 237-246  https://doi.org/10.1007/BF01681816
Lange O (): Isozyme Variation in Wild and Cultivated Species of the Genus Trifolium L. (Leguminosae). Annals of Botany, 86, 339-345  https://doi.org/10.1006/anbo.2000.1190
Malaviya D.R., Kumar B., Roy A.K., Kaushal P., Tiwari A. (2005): Estimation of variability for isozymes of five enzyme systems among wild and cultivated species of Trifolium. Genetic Resources and Crop Evolution, 52: 967–976.
Malaviya D. R., Roy A. K., Kaushal P., Kumar B., Tiwari A. (2008): Genetic similarity among Trifolium species based on isozyme banding pattern. Plant Systematics and Evolution, 276, 125-136  https://doi.org/10.1007/s00606-008-0070-7
Mantel N. (1967): The detection of disease clustering and a generalized regression approach. Cancer Research, 27: 209–220.
Paplauskienė V., Dabkevičienė G. (2012): A study of genetic diversity in Trifolium hybridum varieties using morphological characters and ISSR markers. Žemdirbystė–Agriculture, 99: 313–318.
Quezel P., Santa S. (1962): Nouvelle flore de l’Algérie et des régions désertiques méridionales. CNRS, Paris.
Singh N.K., Shepherd K.W., Cornish G.B. (1991): A simplified SDS-PAGE procedure for separating LMW subunits of glutenin. Journal of Cereal Science, 14: 203–208.
Smith J.S.C., Smith O.S. (1989): The description and assessment of distances between inbred lines of maize: II. The utility of morphological, biochemical and genetic descriptors and a scheme for the testing of distinctiveness between inbred lines. Maydica, 34: 151–161.
Statsoft, Inc. (1995): STATISTICA for Windows. Tulsa, StatSoft, Inc. Available at http://www.statsoftinc.com
Stewart P. (1974): Un nouveau climagramme pour l’Algérie et son application au barrage vert. Bulletin de la Société d’histoire naturelle de l’Afrique du nord, 65: 239–248.
Tucak M., Čupić T., Popović S., Stjepanović M., Gantner R., Meglič V. (2009): Agronomic evaluation and utilization of red clover (Trifolium pratense L.) germplasm. Notulae Botanicae Horti Agrobotanici, 37: 206–210.
Williams W.M., Nichols S.N. (2011): Trifolium. In: Kole C. (ed.): Wild Crop Relatives: Genomic and Breeding Resources. Clemson, Institute of Nutraceutical Research: 249–272.
Zohary M., Heller D. (1984): The genus Trifolium. Jerusalem, Israel Academy of Sciences and Humanities.
Zoro Bi I., Maquet A., Wathelet B., Baudoin J.P. (1999): Genetic control of isozymes in the primary gene pool of Phaseolus lunatus L. Biotechnologie Agronomie Société et Environnement, 3: 10–27.
download PDF

© 2019 Czech Academy of Agricultural Sciences