Citation:Sun J., Wang J., Su D., Yang J., Wang E., Wu S., Li M., Ma L. (2020): Discrimination of tobacco cultivars using SCAR and RAPD markers. Czech J. Genet. Plant Breed., 56: 170−173.
Tobacco genetic purity is crucial to maintain the quality of cigarette products in the tobacco industry. To reduce the difficulties in the discrimination of large number of tobacco cultivars in production practice, we developed a two-step identification strategy by using SCAR and RAPD markers. A total of 53 tobacco cultivars were examined in the study. Initially, all the selected cultivars were divided into four groups, each group consisted of seven to seventeen tobacco cultivars based on difference in phenotypes identified by the SCAR markers S4 and S8. Later, in each group, each tobacco cultivar was identified using RAPD fingerprinting by using one to four polymorphic primers, which were selected from 200 random primers. The results showed that all 53 tobacco cultivars could be effectively distinguished by using only two SCAR and seven RAPD markers. The two-step fingerprinting strategy could be used as a convenient and cost-effective tool to discriminate large numbers of tobacco cultivars for production planning in the tobacco industry.
cultivar; fingerprinting; identification; RAPD; SCAR; Nicotiana tabacum
Chang A.H., Qu Y.S., Jia X.H. (2004): Optimization of tobacco RAPD reaction system and studies on polymorphic marker of tobacco cultivars. Chinese Tobacco Science, 2: 13–16.
Dar A.A., Mahajan R., Sharma S. (2019) Molecular markers for the characterisation and conservation of plant genetic resources. Indian Journal of Agricultural Sciences, 89: 1755–1763.
del Piano L., Abet M., Sorrentino C., Acanfora F., Cozzolino E., Di Muro A. (2014): Genetic variability in Nicotiana tabacum and Nicotiana species as revealed by RAPD markers: 1. development of the RAPD procedure. Beiträge zur Tabakforschung International, 9: 1–15.
Ma L., Luo Z.B., Luo H.Y., Wang S.K., Chang S.R., Rao Z., Dong S.F. (2012): Using SCAR markers to identify tobacco cultivars. Acta Tabacaria Siniaca, 18: 79–84.
Milla S.R., Levin J.S., Lewis R.S., Rufty R.C. (2005): RAPD and SCAR markers linked to an introgressed gene conditioning resistance to Peronospora tabacina D.B. Adam in tobacco. Crop Science, 45: 2346–2354.
https://doi.org/10.2135/cropsci2004.0754
Raju K.S. (2011): Genetic diversity in Indian chewing tobacco (Nicotiana tabacum) as revealed by RAPD and SSR markers. Indian Journal of Agricultural Sciences, 81: 15–19.
Rossi L., Bindler G., Pijnenburg H., Isaac P.G., Giraud-Henry I., Mahe M. (2001): Potential of molecular marker analysis for variety identification in processed tobacco. Plant Cultivars and Seeds, 14: 89–101.
Sun J.P., Wu Z.H., Li X.J., Sun H., Ding Y.F., Ping W.L., Li Y.P. (2016): Analysis of regional variation and major cultivars of flue-cured tobacco planted in China in the twenty-first century. Chinese Tobacco Science, 37: 86–92.
Wang Z.D., Mu J.M., Dai P.G., Wang W.F., Jiang Y.E. (2003): RAPD analysis of some of core germplasms in tobacco. Acta Tabacaria Siniaca, 9: 20–25.
Xu M.H., Zheng M.H., Liu G.T. (1998): Genetic diversity in cultivars of Nicotiana tabacum revealed by RAPD markers. Journal of Agricultural Biotechnology, 6: 281–285.
Yang Y.C., Zhou Q.M., Yin H.Q. (2006): Analysis of genetic diversity in tobacco germplasm by RAPDs and AFLPs. Journal of Agricultural Biotechnology, 14: 585–593.
