Introgression of submergence tolerance into CO 43, a popular rice variety of India, through marker-assisted backcross breeding
Submergence is a major threat in rice growing areas of South and South-East Asia. Identification of a major QTL, Sub1 and advancements in marker-assisted backcrossing strategy enabled breeders to develop submergence tolerant versions of popular rice cultivars. In the present study, a marker-assisted backcross breeding (MABB) approach was used to introgress the Sub1 locus from the tolerant FR13A variety into CO 43, one of the popular rice varieties of southern India. Evaluation of early generation segregating progenies derived from the cross of CO 43 and FR13A revealed the introgression of Sub1, leading to increased tolerance to submergence and better revival ability after it. Genotyping and phenotyping of BC3F3 generation resulted in elite NILs of CO 43 harbouring Sub1 the locus and possessing 94.37% and 95.78% of the recurrent parental CO 43 genome. Selected NILs performed equal as the recurrent parent CO 43 under normal conditions, but were more tolerant to submergence and revived better, afterwards. Under flooding NILs yielded about 25–30% higher than the recurrent parent CO 43.
AICRIP (2015): All India Coordinated Rice Improvement Project. Progress Report 2015. Varietal Improvement. Vol. 1. Hyderabad, ICAR-Indian Institute of Rice Research.
AICRIP (2016): All India Coordinated Rice Improvement Project. Progress Report 2016. Varietal Improvement. Vol. 1. Hyderabad, ICAR-Indian Institute of Rice Research.
Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seid-man J., Smith J.A., Struhl K. (1994): Current Protocols in Molecular Biology. Vol. I. New York, John Wiley & Sons.
Chen Sheng, Lin X.H., Xu C.G., Zhang Qifa (2000): Improvement of Bacterial Blight Resistance of `Minghui 63', an Elite Restorer Line of Hybrid Rice, by Molecular Marker-Assisted Selection. Crop Science, 40, 239- https://doi.org/10.2135/cropsci2000.401239x
Chen , Xu , Lin , Zhang (2001): Improving bacterial blight resistance of '6078', an elite restorer line of hybrid rice, by molecular marker-assisted selection. Plant Breeding, 120, 133-137 https://doi.org/10.1046/j.1439-0523.2001.00559.x
Hossain M., Laborte A. (1996): Differential Growth in Rice Production in Eastern India: Agroecological and Socio-economic Constraints. Physiology of Stress Tolerance of Rice. Los Banos, NDUAT, IRRI: 221–239.
Ismail A., Thomson M., Vergara G., Rahman M., Singh R., Gregorio G., Mackill D. (2010): Designing resilient rice varieties for coastal deltas using modern breeding tools. In: Hoanh C.T., Szuster B., Kam S., Ismail A., Noble A. (eds.): Tropical Deltas and Coastal Zones: Food Production, Communities and Environment at the Land and Water Interface. CAB International: 154–165.
Mohanty H., Chaudhary R. (1986): Breeding for submergence tolerance in rice in India. Progress in Rainfed Lowland Rice. Los Banos, International Rice Research Institute: 191–200.
Neeraja C. N., Maghirang-Rodriguez R., Pamplona A., Heuer S., Collard B. C. Y., Septiningsih E. M., Vergara G., Sanchez D., Xu K., Ismail A. M., Mackill D. J. (2007): A marker-assisted backcross approach for developing submergence-tolerant rice cultivars. Theoretical and Applied Genetics, 115, 767-776 https://doi.org/10.1007/s00122-007-0607-0
Ray Deepak K., Mueller Nathaniel D., West Paul C., Foley Jonathan A., Hart John P. (2013): Yield Trends Are Insufficient to Double Global Crop Production by 2050. PLoS ONE, 8, e66428- https://doi.org/10.1371/journal.pone.0066428
Sarkar R., Reddy J., Sharma S., Ismail A.M. (2006): Physiological basis of submergence tolerance in rice and implications for crop improvement. Current Science, 91: 899–906.
Septiningsih Endang M., Pamplona Alvaro M., Sanchez Darlene L., Neeraja Chirravuri N., Vergara Georgina V., Heuer Sigrid, Ismail Abdelbagi M., Mackill David J. (2009): Development of submergence-tolerant rice cultivars: the Sub1 locus and beyond. Annals of Botany, 103, 151-160 https://doi.org/10.1093/aob/mcn206
Septiningsih Endang M., Hidayatun Nurul, Sanchez Darlene L., Nugraha Yudhistira, Carandang Jerome, Pamplona Alvaro M., Collard Bertrand C. Y., Ismail Abdelbagi M., Mackill David J. (2015): Accelerating the development of new submergence tolerant rice varieties: the case of Ciherang-Sub1 and PSB Rc18-Sub1. Euphytica, 202, 259-268 https://doi.org/10.1007/s10681-014-1287-x
Servin B. (): Optimal Positioning of Markers to Control Genetic Background in Marker-Assisted Backcrossing. Journal of Heredity, 93, 214-217 https://doi.org/10.1093/jhered/93.3.214
Servin B. (2004): Toward a Theory of Marker-Assisted Gene Pyramiding. Genetics, 168, 513-523 https://doi.org/10.1534/genetics.103.023358
Visscher P.M., Haley C.S., Thompson R. (1996): Marker-assisted introgression in backcross breeding programs. Genetics, 144: 1923–1932.
Xu Kenong, Mackill David J. (1996): A major locus for submergence tolerance mapped on rice chromosome 9. Molecular Breeding, 2, 219-224 https://doi.org/10.1007/BF00564199
Xu K., Xu X., Ronald P. C., Mackill D. J. (2000): A high-resolution linkage map of the vicinity of the rice submergence tolerance locus Sub1. Molecular and General Genetics MGG, 263, 681-689 https://doi.org/10.1007/s004380051217
Xu Kenong, Xu Xia, Fukao Takeshi, Canlas Patrick, Maghirang-Rodriguez Reycel, Heuer Sigrid, Ismail Abdelbagi M., Bailey-Serres Julia, Ronald Pamela C., Mackill David J. (2006): Sub1A is an ethylene-response-factor-like gene that confers submergence tolerance to rice. Nature, 442, 705-708 https://doi.org/10.1038/nature04920
Zhou P., Tan Y., He Y., Xu C., Zhang Q. (2003): Simultaneous improvement for four quality traits of Zhenshan 97, an elite parent of hybrid rice, by molecular marker-assisted selection. Theoretical and Applied Genetics, 106, 326-331 https://doi.org/10.1007/s00122-002-1023-0