Mapping QTLs for cold tolerance at seedling stage using an Oryza sativa × O. rufipogon backcross inbred line population

https://doi.org/10.17221/154/2016-CJGPBCitation:Yu S., Li M., Xiao Y., Huang D., Chen D. (2018): Mapping QTLs for cold tolerance at seedling stage using an Oryza sativa × O. rufipogon backcross inbred line population. Czech J. Genet. Plant Breed., 54: 59-64.
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Tolerance to low temperature is an important factor affecting the growth and development of rice (Oryza sativa L.) at an early growing season in the temperate region, and at high altitudes of tropical regions. In this study, a backcross inbred line (BIL) population derived from an interspecific cross between Xieqingzao B (O. sativa L.) and an accession of Dongxiang wild rice (O. rufipogon Griff.) was used to identify quantitative trait loci (QTLs) associated with cold tolerance at the seedling stage. Seedlings were treated with a temperature of 6°C for 2 days and seedling mortality was measured for QTL mapping. QTL analysis was performed on the whole BIL population and on one subpopulation that showed Xieqingzao B homozygous at QTL detected in the whole population. One major QTL, qSCT8, and one QTL, qSCT4.3, with smaller effect was found in the whole population. The QTLs qSCT8 and qSCT4.3 were mapped on chromosome 8 and 4, explaining 60.96% and 8.83% of the phenotypic variance, respectively. In the subpopulation, three QTLs, qSCT4.1, qSCT4.2 and qSCT12, accounting for 56.22%, 57.62% and 53.09% of the phenotypic variance, respectively, were detected on chromosome 4 and 12. At all five loci, the alleles introduced from the Dongxiang wild rice were effective in decreasing seedling mortality. Our results provide a basis for fine mapping and cloning of QTLs associated with cold tolerance, and the markers linked with QTLs could be used to improve the cold tolerance of rice varieties by marker-assisted selection.

References:
Andaya V. C. (2003): Mapping of QTLs associated with cold tolerance during the vegetative stage in rice. Journal of Experimental Botany, 54, 2579-2585  https://doi.org/10.1093/jxb/erg243
 
Andaya V. C., Tai T. H. (2006): Fine mapping of the qCTS12 locus, a major QTL for seedling cold tolerance in rice. Theoretical and Applied Genetics, 113, 467-475  https://doi.org/10.1007/s00122-006-0311-5
 
Chen D.Z., Xiao Y.Q., Zhao S.X., Xiong H.J., Pi Y.H. (1996): Study on the cold tolerance of Dongxiang wildrice at stage of seedling and heading. Acta Agricualturae Jiangxi, 8: 1–6.
 
Chen D.Z., Zhong P.A., Xiao Y.Q., Huang Y.J., Xie J.K. (2002): Identification of QTLs for cold tolerance at seedling stage in Dongxiang wild rice (Oryza rufiongon Griff.) by SSR markers. Acta Agricualturae Universitatis Jiangxiensis, 24: 753–756.
 
Chen Jie, Huang De-Run, Wang Lei, Liu Guang-Jie, Zhuang Jie-Yun (2010): Identification of quantitative trait loci for resistance to whitebacked planthopper, Sogatella furcifera, from an interspecific cross Oryza sativa × O. rufipogon. Breeding Science, 60, 153-159  https://doi.org/10.1270/jsbbs.60.153
 
CHEN Xiao-rong, YANG Kong-song, FU Jun-ru, ZHU Chang-lan, PENG Xiao-song, HE Xiao-peng, HE Hao-hua (2008): Identification and Genetic Analysis of Fertility Restoration Ability in Dongxiang Wild Rice (Oryza rufipogon). Rice Science, 15, 21-28  https://doi.org/10.1016/S1672-6308(08)60015-3
 
Dai Luyuan, Lin Xinghua, Ye Changrong, Ise Kazuo, Saito Koji, Kato Akira, Xu Furong, Yu Tengqiong, Zhang Duanpin (2004): Identification of Quantitative Trait Loci Controlling Cold Tolerance at the Reproductive Stage in Yunnan Landrace of Rice, Kunmingxiaobaigu. Breeding Science, 54, 253-258  https://doi.org/10.1270/jsbbs.54.253
 
Fujino K., Sekiguchi H., Sato T., Kiuchi H., Nonoue Y., Takeuchi Y., Ando T., Lin S. Y., Yano M. (2004): Mapping of quantitative trait loci controlling low-temperature germinability in rice ( Oryza sativa L.). TAG Theoretical and Applied Genetics, 108, 794-799  https://doi.org/10.1007/s00122-003-1509-4
 
He G.C., Shu L.H., Zhou Y.Q., Liao L.J. (1996). The overwintering ability of Dongxiang wild rice (Oryza rufipogon) at Wuhan. Journal of Wuhan University (Natural Science Edition), 42: 252–254.
 
Hu B.-L., Huang D.-R., Xiao Y.-Q., Fan Y.-Y., Chen D.-Z., Zhuang J.-Y. (2016): Mapping QTLs for mineral element contents in brown and milled rice using an Oryza sativa × O. rufipogon backcross inbred line population. Cereal Research Communications, 44, 57-68  https://doi.org/10.1556/0806.43.2015.044
 
IRRI (1988): Standard Evaluation System for Rice. Los Baños, Internationla Rice Research Institute.
 
Kaneda C., Beachell H.M. (1973): Response of indica-japonica rice hybrids to low temperatures. SABRAO Journal, 6: 17–32.
 
Khush Gurdev S (1999): Green revolution: preparing for the 21st century. Genome, 42, 646-655  https://doi.org/10.1139/g99-044
 
Kim Suk-Man, Suh Jung-Pil, Lee Chung-Koon, Lee Jeong-Heui, Kim Yeong-Gyu, Jena Kshirod Kumar (2014): QTL mapping and development of candidate gene-derived DNA markers associated with seedling cold tolerance in rice (Oryza sativa L.). Molecular Genetics and Genomics, 289, 333-343  https://doi.org/10.1007/s00438-014-0813-9
 
Li Fei, Guo Siyi, Zhao Yuan, Chen Dazhou, Chong Kang, Xu Yunyuan (2010): Overexpression of a homopeptide repeat-containing bHLH protein gene (OrbHLH001) from Dongxiang Wild Rice confers freezing and salt tolerance in transgenic Arabidopsis. Plant Cell Reports, 29, 977-986  https://doi.org/10.1007/s00299-010-0883-z
 
LIU Fengxia (2003): Identification and mapping of quantitative trait loci controlling cold-tolerance of Chinese common wild rice (O. rufipogon Griff.) at booting to flowering stages. Chinese Science Bulletin, 48, 2068-  https://doi.org/10.1360/03wc0287
 
McCouch Susan R. (2008): Gene Nomenclature System for Rice. Rice, 1, 72-84  https://doi.org/10.1007/s12284-008-9004-9
 
Nakagahra M., Okuno K., Vaughan D. (1997): Rice genetic resources: history, conservation, investigative characterization and use in Japan. Plant Molecular Biology, 35: 69–77. https://doi.org/10.1023/A:1005784431759
 
Qian Qian, Zeng Dali, He Ping, Zheng Xianwu, Chen Ying, Zhu Lihuang (2000): QTL analysis of the rice seedling cold tolerance in a double haploid population derived from anther culture of a hybrid betweenindica andjaponica rice. Chinese Science Bulletin, 45, 448-453  https://doi.org/10.1007/BF02884949
 
Saito Koji, Hayano-Saito Yuriko, Kuroki Makoto, Sato Yutaka (2010): Map-based cloning of the rice cold tolerance gene Ctb1. Plant Science, 179, 97-102  https://doi.org/10.1016/j.plantsci.2010.04.004
 
Suh J. P., Jeung J. U., Lee J. I., Choi Y. H., Yea J. D., Virk P. S., Mackill D. J., Jena K. K. (2010): Identification and analysis of QTLs controlling cold tolerance at the reproductive stage and validation of effective QTLs in cold-tolerant genotypes of rice (Oryza sativa L.). Theoretical and Applied Genetics, 120, 985-995  https://doi.org/10.1007/s00122-009-1226-8
 
Wang S.C., Basten C.J., Zeng Z.B. (2012): Windows QTL Cartographer 2.5. Department of Statistics. North CarolinaStateUniversity, Raleigh. Availble at http://statgen.ncsu.edu/qtlcart/WQTLCart.htm
 
Wu M.S., Lin D.H. (1990): Research on cold tolerance in wild rice. In: Collection of Research Articles on Wild Rice Germplasm. Beijing, Chinese Science Techn Press: 57–62.
 
Xia R.X., Xiao N., Hong Y.H., Zhang C., Su Y., Zhang X.M., Chen J.M. (2010): QTLs mapping for cold tolerance at seedling stage in Dongxiang wild rice (Oryza rufipogon Griff.). Scientia Agriculture Sinica, 43: 443–451.
 
Xiao Ning, Huang Wei-nan, Zhang Xiao-xiang, Gao Yong, Li Ai-hong, Dai Yi, Yu Ling, Liu Guang-qing, Pan Cun-hong, Li Yu-hong, Dai Zheng-yuan, Chen Jian-min, Xu Mingliang (2014): Fine Mapping of qRC10-2, a Quantitative Trait Locus for Cold Tolerance of Rice Roots at Seedling and Mature Stages. PLoS ONE, 9, e96046-  https://doi.org/10.1371/journal.pone.0096046
 
Xiao N., Huang W.N., Li A.H., Gao Y., Li Y.H., Pan C.H., Ji H.J., Zhang X.X. Dai Y., Dai Z.Y., Chen J.M. ( 2015): Fine mapping of the qLOP2 and qPSR2-1 loci associated with chilling stress tolerance of wild rice seedlings. Theoretical and Applied Genetics, 128: 173–185.
 
Yoshida R., Kanno A., Sato T., Kameya T. (1996): Cool-Temperature-Induced Chlorosis in Rice Plants (I. Relationship between the Induction and a Disturbance of Etioplast Development). Plant Physiology, 110, 997-1005  https://doi.org/10.1104/pp.110.3.997
 
Zhang Q., Chen Q.H., Wang S.L., Hong Y.H., Wang Z.L. (2014): Rice and cold stress: methods for its evaluation and summary of cold tolerance-related quantitative trait loci. Rice (New York, N.Y.), 7: 24.
 
Zhang Xia, Zhou Shaoxia, Fu Yongcai, Su Zhen, Wang Xiangkun, Sun Chuanqing (2006): Identification of a Drought Tolerant Introgression Line Derived from Dongxiang Common Wild Rice (O. rufipogon Griff.). Plant Molecular Biology, 62, 247-259  https://doi.org/10.1007/s11103-006-9018-x
 
ZHANG Z.-H. (2004): Comparison of QTL Controlling Seedling Vigour under Different Temperature Conditions Using Recombinant Inbred Lines in Rice (Oryza sativa). Annals of Botany, 95, 423-429  https://doi.org/10.1093/aob/mci039
 
Zuo J., Gao J., He R.H., Li D., Han X.X., Xing J., Li Q., Cao M.L. (2010): QTL mapping for cold resistance at seedling stage in Dongxiang wild rice. Hybrid Rice, 27: 56–59.
 
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