Enhanced tolerance to low-K+ stress in tobacco plants, that ectopically express the CBL-interacting protein kinase CIPK23 gene
G. Xue, L.-M. Lu, T.-Z. Yang, X.-H. Li, X.-X. Xing, S.-X. Xuhttps://doi.org/10.17221/155/2015-CJGPBCitation:Xue G., Lu L.-., Yang T.-., Li X.-., Xing X.-., Xu S.-. (2016): Enhanced tolerance to low-K+ stress in tobacco plants, that ectopically express the CBL-interacting protein kinase CIPK23 gene. Czech J. Genet. Plant Breed., 52: 77-82.
Tobacco (Nicotiana tabacum) has a relatively high requirement for potassium (K+). However, the molecular basis of tolerance to low-K+ stresses in tobacco still remains unknown. Here, we report the role of a member of the A. thaliana CBL (calcineurin B-like) interacting protein kinase (CIPK) family, AtCIPK23, in low-K+ stress responses in tobacco. Molecular analyses revealed that the AtCIPK23 gene was successfully transferred into a tobacco cultivar K326 via Agrobacterium tumefaciens-mediated transformation. Overexpression of AtCIPK23 in tobacco resulted in increased low-K+ tolerance, which was demonstrated by higher dry biomass, longer primary root length, higher K+ content and better growth status of transgenic tobacco plants compared to controls when both were treated in low-K+ MS medium and low-K+ hydroponics. Moreover, transgenic lines conferred tolerance to low-K+ stress by increasing the K+ uptake rate under low-K+ conditions. Taken together, these results provide evidence that AtCIPK23 may be involved in the CBL-CIPK signalling network in tobacco responses to low-K+ stress.Keywords:
AtCIPK23; K+ uptake; low-K+ tolerance; potassium; tobaccoReferences:
Ashley M. K. (2005): Plant responses to potassium deficiencies: a role for potassium transport proteins. Journal of Experimental Botany, 57, 425-436 https://doi.org/10.1093/jxb/erj034Cheong Yong Hwa, Pandey Girdhar K., Grant John J., Batistic Oliver, Li Legong, Kim Beom-Gi, Lee Sung-Chul, Kudla Jörg, Luan Sheng (2007): Two calcineurin B-like calcium sensors, interacting with protein kinase CIPK23, regulate leaf transpiration and root potassium uptake in Arabidopsis. The Plant Journal, 52, 223-239 https://doi.org/10.1111/j.1365-313X.2007.03236.xCherel I., Lefoulon C., Boeglin M., Sentenac H. (): Molecular mechanisms involved in plant adaptation to low K+ availability. Journal of Experimental Botany, 65, 833-848 https://doi.org/10.1093/jxb/ert402CuÃ©llar Teresa, Pascaud FranÃ§ois, Verdeil Jean-Luc, Torregrosa Laurent, Adam-Blondon Anne-FranÃ§oise, Thibaud Jean-Baptiste, Sentenac HervÃ©, Gaillard Isabelle (2010): A grapevine Shaker inward K + channel activated by the calcineurin B-like calcium sensor 1âprotein kinase CIPK23 network is expressed in grape berries under drought stress conditions. The Plant Journal, 61, 58-69 https://doi.org/10.1111/j.1365-313X.2009.04029.xDeng X.M., Hu W., Wei S.Y., Zhou S.Y., Zhang F., Han J.P., Chen L.H., Li Y., Feng J.L., Fang B., Luo Q.C., Li S.S., Liu Y.Y., Yang G.X., He G.Y. (2013a): TaCIPK29, a CBL-Interacting protein kinase gene from wheat, confers salt stress tolerance in transgenic tobacco. PLoS ONE, 8: e69881.Deng X.M., Zhou S.Y., Hu W., Feng J.L., Zhang F., Chen L.H., Huang C., Luo Q.C., He Y.Z., Yang G.X., He G.Y. (2013b): Ectopic expression of wheat TaCIPK14, encoding a calcineurin B-like protein-interacting protein kinase, confers salinity and cold tolerance in tobacco. Physiologia Plantarum, 149: 367–377.He Liangrong, Yang Xiyan, Wang Lichen, Zhu Longfu, Zhou Ting, Deng Jinwu, Zhang Xianlong (2013): Molecular cloning and functional characterization of a novel cotton CBL-interacting protein kinase gene (GhCIPK6) reveals its involvement in multiple abiotic stress tolerance in transgenic plants. Biochemical and Biophysical Research Communications, 435, 209-215 https://doi.org/10.1016/j.bbrc.2013.04.080Hirsch R. E. (): A Role for the AKT1 Potassium Channel in Plant Nutrition. Science, 280, 918-921 https://doi.org/10.1126/science.280.5365.918(1985): A Simple and General Method for Transferring Genes into Plants. Science, 227, 1229-1231 https://doi.org/10.1126/science.227.4691.1229LEIGH R. A., WYN JONES R. G. (1984): A HYPOTHESIS RELATING CRITICAL POTASSIUM CONCENTRATIONS FOR GROWTH TO THE DISTRIBUTION AND FUNCTIONS OF THIS ION IN THE PLANT CELL. New Phytologist, 97, 1-13 https://doi.org/10.1111/j.1469-8137.1984.tb04103.xLi J., Long Y., Qi G.-N., Li J., Xu Z.-J., Wu W.-H., Wang Y. (): The Os-AKT1 Channel Is Critical for K+ Uptake in Rice Roots and Is Modulated by the Rice CBL1-CIPK23 Complex. The Plant Cell, , - https://doi.org/10.1105/tpc.114.123455Li R.F., Zhang J.W., Wu G.Y., Wang H.Z., Chen Y.J., Wei J.H. (2012): HbCIPK2, a novel CBL-interacting protein kinase from halophyte Hordeum brevisubulatum, confers salt and osmotic stress tolerance. Plant, Cell & Environment, 35: 1582–1600.Shabala Sergey, Pottosin Igor (2014): Regulation of potassium transport in plants under hostile conditions: implications for abiotic and biotic stress tolerance. Physiologia Plantarum, 151, 257-279 https://doi.org/10.1111/ppl.12165(): Strategies for Improving Potassium Use Efficiency in Plants. Molecules and Cells, , - https://doi.org/10.14348/molcells.2014.0141Tai Fuju, Wang Qi, Yuan Zuli, Yuan Zhiheng, Li Huiyun, Wang Wei (2013): Characterization of five CIPK genes expressions in maize under water stress. Acta Physiologiae Plantarum, 35, 1555-1564 https://doi.org/10.1007/s11738-012-1197-2Tripathi Vineeta, Parasuraman Boominathan, Laxmi Ashverya, Chattopadhyay Debasis (2009): CIPK6, a CBL-interacting protein kinase is required for development and salt tolerance in plants. The Plant Journal, 58, 778-790 https://doi.org/10.1111/j.1365-313X.2009.03812.xWang Xiyao, Li Jia, Zou Xue, Lu Liming, Li Liqin, Ni Su, Liu Fan (2011): Ectopic Expression of AtCIPK23 Enhances Tolerance Against Low-K+ Stress in Transgenic Potato. American Journal of Potato Research, 88, 153-159 https://doi.org/10.1007/s12230-010-9173-0Wang Rong-Kai, Li Ling-Li, Cao Zhong-Hui, Zhao Qiang, Li Ming, Zhang Ling-Yun, Hao Yu-Jin (2012): Molecular cloning and functional characterization of a novel apple MdCIPK6L gene reveals its involvement in multiple abiotic stress tolerance in transgenic plants. Plant Molecular Biology, 79, 123-135 https://doi.org/10.1007/s11103-012-9899-9Wang Yi, Wu Wei-Hua (2013): Potassium Transport and Signaling in Higher Plants. Annual Review of Plant Biology, 64, 451-476 https://doi.org/10.1146/annurev-arplant-050312-120153Xu Jiang, Li Hao-Dong, Chen Li-Qing, Wang Yi, Liu Li-Li, He Liu, Wu Wei-Hua (2006): A Protein Kinase, Interacting with Two Calcineurin B-like Proteins, Regulates K+ Transporter AKT1 in Arabidopsis. Cell, 125, 1347-1360 https://doi.org/10.1016/j.cell.2006.06.011Yang Wenqiang, Kong Zhaosheng, Omo-Ikerodah Edith, Xu Wenying, Li Qun, Xue Yongbiao (2008): Calcineurin B-like interacting protein kinase OsCIPK23 functions in pollination and drought stress responses in rice (Oryza sativa L.). Journal of Genetics and Genomics, 35, 531-S2 https://doi.org/10.1016/S1673-8527(08)60073-9Yu Yanhua, Xia Xinli, Yin Weilun, Zhang Hechen (2007): Comparative genomic analysis of CIPK gene family in Arabidopsis and Populus. Plant Growth Regulation, 52, 101-110 https://doi.org/10.1007/s10725-007-9165-3Zhang Hechen, Yin Weilun, Xia Xinli (2010): Shaker-like potassium channels in Populus, regulated by the CBL–CIPK signal transduction pathway, increase tolerance to low-K+ stress. Plant Cell Reports, 29, 1007-1012 https://doi.org/10.1007/s00299-010-0886-9