Marker characterization of vernalization and low-temperature tolerance loci in barley genotypes adapted to semi-arid environments

https://doi.org/10.17221/16/2016-CJGPBCitation:Benkherbache N., Tondelli A., Djekoune A., Francia E., Pecchioni N., Hassous L., Stanca A.M. (2016): Marker characterization of vernalization and low-temperature tolerance loci in barley genotypes adapted to semi-arid environments. Czech J. Genet. Plant Breed., 52: 157-162.
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For the purpose of marker assisted selection (MAS), six Algerian cultivars and landraces developed locally or introduced from external breeding programs for semi-arid environments were marker characterized at VRN-H1, VRN-H2 and FR-H2 loci. As controls the lines Nure and Tremois were used. Markers available in the literature, known to discriminate efficiently the trait-associated alleles between the two cultivars Nure and Tremois, were used. This study revealed that the used Algerian cultivars carry a dominant (spring) allele for VRN-H1, while the HvZCCT marker generated a polymorphic profile for the second vernalization locus VRN-H2. No cultivars possessed the Nure allele conferring tolerance to low-temperatures at the HvCBF4. Three cultivars possessed the Tremois allele at FR-H2, while the remaining three presented different haplotypes.

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