Interspecific crosses play an important role in gene introgression, plant improvement and speciation. However, poor fertility of F1 generation was commonly found, which hampered backcrossing and ideal progeny generation. To explore useful materials for further breeding programs, sterile hybrids (DD1, FD1, ZD3) from different cross combinations of Lagerstroemia indica and L. speciosa and the fertile hybrid (ZD6) were selected. The results showed that pollen grains of sterile hybrids had no germination ability while ZD6 showed 25.90% pollen germination rate. The morphology of stigmas and their papilla cells showed no apparent difference. Normal pollen tubes could be detected in ovaries of ZD6 and ZD3 24 h after pollination. However, the enlarged ovaries of ZD3 began to abscise at 72 h after pollination, which suggested that the barriers occurred during post-fertilization phases. As a consequence, ZD6 can be used as either male or female parent for further Lagerstroemia breeding programs, while the sterile hybrids may be used as female parent through embryo rescue culture.
Cai M., Wang X.Y., Zhang Q.X., Pan H.T., Wang X.F. (2010): Compatibility of interspecific crosses between Lagerstroemia indica cultivars and Lagerstroemia caudata. Acta Botanica Boreali-Occidentalia Sinica, 30: 645–651. (in Chinese)
Carroll R.H. (2008): Lagerstroemia Plant Named ‘Trured’. United States Plant Patent, US PP18, 456.
Chu Pu, Liu Huijuan, Yang Qing, Wang Yankun, Yan Guixia, Guan Rongzhan (2014): An RNA-seq transcriptome analysis of floral buds of an interspecific Brassica hybrid between B. carinata and B. napus. Plant Reproduction, 27, 225-237
https://doi.org/10.1007/s00497-014-0253-z
CLAESSON L., KOTIMÄKI M., BOTHMER R. VON (1990): Crossability and chromosome pairing in some interspecific Triticum hybrids. Hereditas, 112, 49-55
https://doi.org/10.1111/j.1601-5223.1990.tb00136.x
Egolf D.R. (1987): ‘Biloxi’, ‘Miami’ and ‘Wichita’ Lagerstroemia. HortScience, 22: 336–338.
WEDDERBURN FRANCES, RICHARDS A. J. (1990): Variation in within-morph incompatibility inhibition sites in heteromorphic Primula L.. New Phytologist, 116, 149-162
https://doi.org/10.1111/j.1469-8137.1990.tb00520.x
Furtado C.X., Srisuko M. (1969): A Revision of Lagerstroemia L. (Lythraceae). Gardens’ Bulletin, Vol. 24, Singapore, Botanic Gardens: 185–334.
Graham S.A., Cavalcanti T.B. (2001): New chromosome counts in Lythraceae and a review of chromosome numbers in the family. Systematic Botany, 26: 445–458.
Kuligowska Katarzyna, Lütken Henrik, Christensen Brian, Skovgaard Ib, Linde Marcus, Winkelmann Traud, Müller Renate (2015): Evaluation of reproductive barriers contributes to the development of novel interspecific hybrids in the Kalanchoë genus. BMC Plant Biology, 15, 15-
https://doi.org/10.1186/s12870-014-0394-0
Naredo M.E.B., Juliano A.B., Lu B.R., Jackson M.T. (2003): The taxonomic status of the wild rice species Oryza ridleyi Hook. f. and O. longiglumis Jansen (Ser. Ridleyanae Sharma et Shastry) from Southeast Asia. Genetic Resources and Crop Evolution, 50: 477–488.
https://doi.org/10.1023/A:1023958130087
Pounders Cecil, Rinehart Tim, Sakhanokho Hamidou (2007): Evaluation of Interspecific Hybrids between Lagerstroemia indica and L. speciosa. HortScience, 42, 1317-1322
https://doi.org/10.21273/HORTSCI.42.6.1317
Rieseberg L. H., Willis J. H. (2007): Plant Speciation. Science, 317, 910-914
https://doi.org/10.1126/science.1137729
Smyth D. R. (): Early Flower Development in Arabidopsis. THE PLANT CELL ONLINE, 2, 755-767
https://doi.org/10.1105/tpc.2.8.755
Wang X.W., Genovesi A., Pounders C., Cabrera R.I. (2010): A preliminary report on the use of embyro rescue techniques with intra-and interspecific hybrids in crape myrtle (Lagerstroemia L.). HortScience, 45: 122–123.
Wang Xinwang, Wadl Phillip A., Pounders Cecil, Trigiano Robert N., Cabrera Raul I., Scheffler Brian E., Pooler Margaret, Rinehart Timothy A. (2011): Evaluation of Genetic Diversity and Pedigree within Crapemyrtle Cultivars Using Simple Sequence Repeat Markers. Journal of the American Society for Horticultural Science, 136, 116-128
https://doi.org/10.21273/JASHS.136.2.116
Xu W. (2014): Breeding in Fragrance and Improvement of Significant Ornamental Traits of Lagerstroemia. Beijing: Beijing Forestry University. (in Chinese)
Yang B.J., Chen J.X., Tang W., Wang X.J., Liu Y., Pan H.T. (2012): Optimization of chromosome sectioning of Lagerstroemia. Guangdong Agricultural Sciences, 17: 135–142. (in Chinese)
Zhang Z.Y., Pang X.M., Han J.W., Wang Y., Li Y.Y. (2013): Conservation genetics of Annamocarya sinensis (Dode) Leroy, an endangered endemic species. Genetics and Molecular Research, 12, 3965-3974
https://doi.org/10.4238/2013.March.11.9
