Molecular markers within or close to genes of interest play essential roles in marker-assisted selection. PCR-based markers have been developed for numerous traits in different plant species including several genes conferring resistance to viruses in potato. In the present work, rapid and reliable approaches were developed for the simultaneous detection of Ryadg and Ry-fsto, Ns, and PLRV.1 genes conferring resistance to Potato virus Y, Potato virus S and Potato leafroll virus, respectively, on the basis of previously published and newly modified markers. The sequence characterized amplified region (SCAR) markers for Ryadg, Ns and PLRV1 and the newly modified cleaved amplified polymorphic sequences (CAPS) marker for Ry-fsto were amplified in one PCR reaction which could simply characterize Ryadg and PLRV.1 resistance. Additional digestion of amplicons with EcoRV and MfeI for genotyping the Ry-fsto and Ns resistance genes, respectively, was needed. The effectiveness of genotyping in triplex and tetraplex PCRs was tested on 35 potato varieties used for potato seed production and breeding programs.
Brigneti G., Garcia-Mas J., Baulcombe D. C. (1997): Molecular mapping of the potato virus Y resistance gene Ry sto in potato. TAG Theoretical and Applied Genetics, 94, 198-203 https://doi.org/10.1007/s001220050400
Flis Bogdan, Hennig Jacek, Strzelczyk-Żyta Danuta, Gebhardt Christiane, Marczewski Waldemar (2005): The Ry-fsto gene from Solanum stoloniferum for extreme resistant to Potato virus Y maps to potato chromosome XII and is diagnosed by PCR marker GP122718 in PVY resistant potato cultivars. Molecular Breeding, 15, 95-101 https://doi.org/10.1007/s11032-004-2736-3
Kasai K, Morikawa Y, Sorri V A, Valkonen JPT, Gebhardt C, Watanabe K N (2000): Development of SCAR markers to the PVY resistance gene Ryadg
based on a common feature of plant disease resistance genes. Genome, 43, 1-8 https://doi.org/10.1139/g99-092
Lopez-Pardo Raquel, Barandalla Leire, Ritter Enrique, Ruiz de Galarreta Jose I., Wehling P. (2013): Validation of molecular markers for pathogen resistance in potato. Plant Breeding, 132, 246-251 https://doi.org/10.1111/pbr.12062
Marczewski W., Talarczyk A., Hennig J. (2001a): Development of SCAR markers linked to the Ns locus in potato. Plant Breeding, 120: 88–90.
Marczewski W., Flis B., Syller J., Schafer-Pregl R., Gebhardt C. (2001b): A major quantitative trait locus for resistance to Potato leaf roll virus is located in a resistance hotspot on potato chromosome XI and is tightly linked to N-gene-like markers. Molecular Plant-Microbe Interactions, 14: 1420–1425.
W. Marczewski, J. Hennig, C. Gebhardt (2002): The Potato virus S resistance gene Ns maps to potato chromosome VIII. TAG Theoretical and Applied Genetics, 105, 564-567 https://doi.org/10.1007/s00122-002-0976-3
Ortega Felisa, Lopez-Vizcon Claudia (2012): Application of Molecular Marker-Assisted Selection (MAS) for Disease Resistance in a Practical Potato Breeding Programme. Potato Research, 55, 1-13 https://doi.org/10.1007/s11540-011-9202-5
Ottoman Ryon J., Hane Dan C., Brown Charles R., Yilma Solomon, James Steven R., Mosley Alvin R., Crosslin James M., Vales M. Isabel (2009): Validation and Implementation of Marker-Assisted Selection (MAS) for PVY Resistance (Ry adg gene) in a Tetraploid Potato Breeding Program. American Journal of Potato Research, 86, 304-314 https://doi.org/10.1007/s12230-009-9084-0
Poczai Péter, Varga Ildikó, Laos Maarja, Cseh András, Bell Neil, Valkonen Jari PT, Hyvönen Jaakko (2013): Advances in plant gene-targeted and functional markers: a review. Plant Methods, 9, 6- https://doi.org/10.1186/1746-4811-9-6
Ramakrishnan Alisa P., Ritland Carol E., Blas Sevillano Raul H., Riseman Andrew (2015): Review of Potato Molecular Markers to Enhance Trait Selection. American Journal of Potato Research, 92, 455-472 https://doi.org/10.1007/s12230-015-9455-7
Saghai-Maroof M. A., Soliman K. M., Jorgensen R. A., Allard R. W. (1984): Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics.. Proceedings of the National Academy of Sciences, 81, 8014-8018 https://doi.org/10.1073/pnas.81.24.8014
Swiezynski K.M. (1994): Inheritance of resistance to viruses. In: Bradshaw J.E., Mackay G.R. (eds.): Potato Genetics. Wallingford, CAB International: 339–363.
Tiwari J.K., Gopal J., Singh B.P. (2012): Marker-assisted selection for virus resistance in potato: options and challenges. Potato Journal, 39: 101–117.
Xu Yunbi, Crouch Jonathan H. (2008): Marker-Assisted Selection in Plant Breeding: From Publications to Practice. Crop Science, 48, 391- https://doi.org/10.2135/cropsci2007.04.0191