Genetic effects of F1 pollen sterility genes S-b, S-d and S-e in rice (Oryza sativa L.) M., Xu J., Chen F., Zhu W. (2019): Genetic effects of F1 pollen sterility genes S-b, S-d and S-e in rice (Oryza sativa L.). Czech J. Genet. Plant Breed., 55: 55-60.
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An experimental population commonly used in genetic analyses of gene or quantitative trait loci (QTLs) in rice is chromosome segment substitution lines (CSSLs). In the present study, with the typical indica variety Guangluai 4 as a donor and japonica variety Taichung 65 as a recipient, seven CSSLs carrying F1 pollen sterility genes S-b, S-d, S-e, S-b/S-d, S-b/S-e, S-d/S-e, and S-b/S-d/S-e were obtained by specific selection for the target genes, non-specific selection for the genome of the recurrent parents in four backcross populations (BC1F2, BC2F2, BC3F2 and BC3F3). We evaluated the genetic effect of the F1 pollen sterility genes using 35 F1 hybrid individuals in crosses derived from CSSLs and Taichung 65. Pollen fertility of F1 hybrid plants was observed and the results indicated that the single genes S-b, S-d and S-e can cause 67.7%, 14.6% and 53.2% of pollen sterility, respectively. Multiple genes S-b/S-d, S-b/S-e, S-d/S-e, and S-b/S-d/S-e can cause 76.6%, 85%, 68.7%, and 93% of pollen sterility, respectively.


Ali M. Liakat, Sanchez Paul L., Yu Si-bin, Lorieux Mathias, Eizenga Georgia C. (2010): Chromosome Segment Substitution Lines: A Powerful Tool for the Introgression of Valuable Genes from Oryza Wild Species into Cultivated Rice (O. sativa). Rice, 3, 218-234
Chen J., Ding J., Ouyang Y., Du H., Yang J., Cheng K., Zhao J., Qiu S., Zhang X., Yao J., Liu K., Wang L., Xu C., Li X., Xue Y., Xia M., Ji Q., Lu J., Xu M., Zhang Q. (2008): A triallelic system of S5 is a major regulator of the reproductive barrier and compatibility of indica-japonica hybrids in rice. Proceedings of the National Academy of Sciences, 105, 11436-11441
Ding X., Chen Y., Yang C., Lu Y., Zhang G. (2002): Test on compatibility of indica-compatible japonica lines in rice. Chinese Journal of Rice Science, 16: 366–368. (in Chinese with English abstract)
Guo Jie, Xu Xiaomei, Li Wentao, Zhu Wenyin, Zhu Haitao, Liu Ziqiang, Luan Xin, Dai Ziju, Liu Guifu, Zhang Zemin, Zeng Ruizhen, Tang Guang, Fu Xuelin, Wang Shaokui, Zhang Guiquan (2016): Overcoming inter-subspecific hybrid sterility in rice by developing indica-compatible japonica lines. Scientific Reports, 6, -
IKEHASHI Hiroshi, ARAKI Hitoshi (1984): Varietal screening of compatibility types revealed in F1 fertility of distant crosses in rice.. Ikushugaku zasshi, 34, 304-313
Ikehashi H., Araki H. (1986): Genetics of F1 sterility in remote cross of rice (Oryza sativa L.). In: Banta J.S. (ed.): Rice Genetics I, Part 2. IRRI: 119–130.
Ikehashi Hiroshi, Araki Hitoshi (1988): Multiple alleles controlling F1 sterility in remote crosses of rice (Oryza sativa).. Ikushugaku zasshi, 38, 283-291
Jing Wen, Zhang Wenwei, Jiang Ling, Chen Liangming, Zhai Huqu, Wan Jianmin (2007): Two novel loci for pollen sterility in hybrids between the weedy strain Ludao and the Japonica variety Akihikari of rice (Oryza sativa L.). Theoretical and Applied Genetics, 114, 915-925
Li W., Zeng R., Zhang Z., Zhang G. (2002): Mapping of S-b locus for F1 pollen sterility in cultivated rice using PCR based markers. Acta Botanica Sinica, 44: 463–467.
Li Wentao, Zeng Ruizhen, Zhang Zemin, Ding Xiaohua, Zhang Guiquan (2006): Fine mapping of locus S-b for F1 pollen sterility in rice (Oryza sativa L.). Chinese Science Bulletin, 51, 675-680
Liu K. D., Wang J., Li H. B., Xu C. G., Liu A. M., Li X. H., Zhang Q. (1997): A genome-wide analysis of wide compatibility in rice and the precise location of the S5 locus in the molecular map. Theoretical and Applied Genetics, 95, 809-814
Liu Y. S., Zhu L. H., Sun J. S., Chen Y. (2001): Mapping QTLs for defective female gametophyte development in an inter-subspecific cross in Oryza sativa L.. Theoretical and Applied Genetics, 102, 1243-1251
Long Y., Zhao L., Niu B., Su J., Wu H., Chen Y., Zhang Q., Guo J., Zhuang C., Mei M., Xia J., Wang L., Wu H., Liu Y.-G. (2008): Hybrid male sterility in rice controlled by interaction between divergent alleles of two adjacent genes. Proceedings of the National Academy of Sciences, 105, 18871-18876
McCouch S. R. (2002): Development and Mapping of 2240 New SSR Markers for Rice (Oryza sativa L.). DNA Research, 9, 199-207
Murray M.G., Thompson W.F. (1980): Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8, 4321-4326
Oka Hiko-Ichi (1957): Genic analysis for the sterility of hybrids between distantly related varieties of cultivated rice. Journal of Genetics, 55, 397-409
Oka H. (1974): Analysis of genes controlling F1 sterility in rice by the use of isogenic lines. Genetics, 77: 521–534.
Panaud O., Chen X., McCouch S. (1996): Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.). Molecular Genetics and Genomics, 252: 597–600.
Qiu S. Q., Liu Kede, Jiang J. X., Song X., Xu C. G., Li X. H., Zhang Qifa (2005): Delimitation of the rice wide compatibility gene S5 n to a 40-kb DNA fragment. Theoretical and Applied Genetics, 111, 1080-1086
Ramos J.M., Furuta T., Uehara K., Chihiro N., Angeles-Shim R.B., Shim J., Brar D.S., Ashikari M., Jena K.K. (2016): Development of chromosome segment substitution lines (CSSLs) of Oryza longistaminata A. Chev. & Röhr in the background of the elite japonica rice cultivar, Taichung 65 and their evaluation for yield traits. Euphytica, 210: 151–163.
Song Xiang, Qiu S. Q., Xu C. G., Li X. H., Zhang Qifa (2005): Genetic dissection of embryo sac fertility, pollen fertility, and their contributions to spikelet fertility of intersubspecific hybrids in rice. Theoretical and Applied Genetics, 110, 205-211
Wan J., Ikehashi H. (1995): Identifecation of a new locus S-16 causing hybrid sterility in native rice varieties (Oryza sativa L.) from Taihu lake region and Yunnan province. Breeding Science, 45: 461–470.
Jianming WAN, YANAGIHARA Seiji, KATO Hiroshi, IKEHASHI Hiroshi (1993): Multiple Alleles at a New Locus Causing Hybrid Sterility between a Korean Indica Variety and a Javanica Variety in Rice (Oryza sativa L.).. Ikushugaku zasshi, 43, 507-516
Wan J., Yamaguchi Y., Kato H., Ikehashi H. (1996): Two new loci for hybrid sterility in cultivated rice (Oryza sativa L.). Theoretical and Applied Genetics, 92, 183-190
Wang J., Liu K. D., Xu C. G., Li X. H., Zhang Q. (1998): The high level of wide-compatibility of variety ‘Dular’ has a complex genetic basis. Theoretical and Applied Genetics, 97, 407-412
YANG Cunyi (2004): Genetic and physical fine-mapping of the Sc locus conferring indica-japonica hybrid sterility in rice (Oryza sativa L.). Chinese Science Bulletin, 49, 1718-
Zhang G., Lu Y. (1989): Genetic studies of the hybrid sterility in cultivated rice (Oryza sativa). I. Diallel analysis of the hybrid sterility among isogenic F1 sterile lines. Chinese Journal of Rice Science, 3: 97–101. (in Chinese with English abstract)
Zhang G., Lu Y. (1993): Genetic studies of the hybrid sterility in cultivated rice (Oryza sativa). II. A genic model for F1 pollen sterility. Acta Genetica Sinica, 20: 222–228. (in Chinese with English abstract)
Zhang Z., Zhang G. (2001): Fine mapping of the S-c locus and marker-assisted selection using PCR markers in rice. Acta Agronomica Sinica, 27: 704–709. (in Chinese with English abstract)
Zhu W., Li., Ding X., Zhang Z., Zeng R., Zhu H., Zhang G. (2008): Preliminary identification of F1 pollen sterility gene S-e in Oryza sativa. Journal of South China Agricultural University, 29: 1–5. (in Chinese with English abstract)
Zhu Wenyin, Lin Jing, Yang Dewei, Zhao Ling, Zhang Yadong, Zhu Zhen, Chen Tao, Wang Cailin (2009): Development of Chromosome Segment Substitution Lines Derived from Backcross between Two Sequenced Rice Cultivars, Indica Recipient 93-11 and Japonica Donor Nipponbare. Plant Molecular Biology Reporter, 27, 126-131
Zhuang C., Fu Y., Zhang G., Mei M., Lu Y. (2002): Molecular mapping of S-c, an F1 pollen sterility gene in cultivated rice. Euphytica, 127: 133–138.
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