Molecular mapping of QTLs for resistance to early and late Fusarium wilt in chickpea
B.S. Patil, R.L. Ravikumar, J.S. Bhat, C.D. Soregaonhttps://doi.org/10.17221/188/2013-CJGPBCitation:Patil B.S., Ravikumar R.L., Bhat J.S., Soregaon C.D. (2014): Molecular mapping of QTLs for resistance to early and late Fusarium wilt in chickpea. Czech J. Genet. Plant Breed., 50: 171-176.
A molecular map of chickpea was constructed using F9:F10 recombinant inbred lines from an intraspecific cross between Fusarium wilt susceptible (JG 62) and resistant (WR 315) genotypes. A total of 23 markers with LOD scores of > 3.0 were mapped on the recombinant inbred lines (RILs). Twenty sequence tagged microsatellites (STMSs) and three amplified fragment length polymorphisms (AFLPs) covered 300.2 cM in five linkage groups at an average inter-marker distance of 13 cM. Early and late wilting due to Fusarium infection was recorded in RILs at 30 and 60 DAS, respectively. There was a significant variation among RILs for wilt resistance for both early and late wilting. QTLs associated with early (30 days after sowing (DAS)) and late (60 DAS) wilting are located on LG II. The flanking markers for these QTLs were the same as those of previous reports. Five STMS markers located on LG II of reference map (interspecific) were mapped on LG II of the present map (intraspecific) with minor changes in the order of markers indicating the conservation of these genomic regions across the Cicer species.Keywords:
chickpea; Fusarium wilt; QTL; STMS