The effect of magnetic field strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.) M., Beyaz R., Bahadir A., Yildiz M. (2019): The effect of magnetic field strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.). Czech J. Genet. Plant Breed., 55: 20-27.
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This study was conducted to determine the effects of magnetic field (MF) strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.). Seeds of flax cv. Madaras were exposed to different MF strengths (0 – control, 75, 150, and 300 millitesla (mT)) for 24 h by using an electromagnetic generator system fabricated in laboratory conditions. After sterilization, seeds were germinated on MS (Murashige and Skoog) medium in Magenta vessels. Hypocotyl explants excised from 7-days-old seedlings were used for regeneration. GV2260 strain of Agrobacterium tumefaciens was used in transformation studies. Inoculated hypocotyls were cultured on MS medium containing 1 mg/l BAP (6-benzylaminopurine) and 0.02 mg/l NAA (naphthaleneacetic acid) for 2 days by co-cultivation. Then, they were transferred to MS medium containing the same growth regulators, 100 mg/l kanamycin and 500 mg/l Duocid for selection. The presence of the nptII gene was verified by PCR (polymerase chain reaction) analysis in putative transgenic plants. The highest results with respect to shoot regeneration and transformation frequency were obtained from treatments of 75 mT MF strength.


Atak C., Emiroglu O., Alikamanoglu S., Rzakoulive A. (2003): Stimulation of regeneration by magnetic field in soybean (Glycine max L. Merrill) tissue cultures. Journal of Cell and Molecular Biology, 2: 113–119.
Belyavskaya N.A., Fomicheva V.M., Govorun R.D., Danilov V.I. (1992): Structural-functional organization of the meristem cells of pea, lentin and flax roots in conditions of screening the geomagnetic field. Biophysics, 37: 657–666.
Carbonnel M.V., Martínez E., Flórez M., Maqueda R., Pintor-López A., Amaya J.M. (2008): Magnetic field treatments improve germination and seedling growth in Festuca arundinacea Schreb. and Lolium perenne L.. Seed Science and Technology, 36, 31-37
Chakrabarty R., Viswakarma N., Bhat S. R., Kirti P. B., Singh B. D., Chopra V. L. (2002): Agrobacterium-mediated transformation of cauliflower: Optimization of protocol and development of Bt-transgenic cauliflower. Journal of Biosciences, 27, 495-502
Dayal S., Shing R.P. (1986): Effect of seed exposure to magnetic field on the height of tomato plants. Indian Journal of Agricultural Science, 56: 483–486.
De Clercq J., Zambre M., Van Montagu M., Dillen W., Angenon G. (2002): An optimized Agrobacterium -mediated transformation procedure for Phaseolus acutifolius A. Gray. Plant Cell Reports, 21, 333-340
Flórez M., Carbonell M. V., Martínez E. (2009): Early Sprouting and First Stages of Growth of Rice Seeds Exposed to a Magnetic Field. Electromagnetic Biology and Medicine, 23, 157-166
FLOREZ M, CARBONELL M, MARTINEZ E (2007): Exposure of maize seeds to stationary magnetic fields: Effects on germination and early growth. Environmental and Experimental Botany, 59, 68-75
Funk Richard H.W., Monsees Thomas, Özkucur Nurdan (2009): Electromagnetic effects – From cell biology to medicine. Progress in Histochemistry and Cytochemistry, 43, 177-264
Galland Paul, Pazur Alexander (2005): Magnetoreception in plants. Journal of Plant Research, 118, 371-389
Goodman E.M., Greenebaum B., Marron M.T. (1995): Effects of electromagnetic fields on molecules and cells. International Review of Cytology, 158: 279–338.
Harris Sue-Re, Henbest Kevin B., Maeda Kiminori, Pannell John R., Timmel Christiane R., Hore P.J., Okamoto Haruko (2009): Effect of magnetic fields on cryptochrome-dependent responses in Arabidopsis thaliana. Journal of The Royal Society Interface, 6, 1193-1205
Heberle-Bors E.F., Moreno R.M.B., Alwen A., Stoger E., Vicente O. (1990): Transformation of pollen. In: Nijkamp H.J.J., Van Der Plas L.H.W., Van Aartrijk J. (eds.): Progress in Plant Cellular and Molecular Biology. Kluwer Academic Publications: 244–251.
Hooykaas Paul J. J., Schilperoort Rob A. (1992): Agrobacterium and plant genetic engineering. Plant Molecular Biology, 19, 15-38
Jordan Mark C., McHughen Alan (1988): Glyphosate tolerant flax plants from Agrobacterium mediated gene transfer. Plant Cell Reports, 7, 281-284
Joubert Philippe, Beaupère Daniel, Lelièvre Philippe, Wadouachi Anne, Sangwan Rajbir S, Sangwan-Norreel Brigitte S (2002): Effects of phenolic compounds on Agrobacterium vir genes and gene transfer induction—a plausible molecular mechanism of phenol binding protein activation. Plant Science, 162, 733-743
Lopez S. Joseph, Kumar R. Raj, Pius P. K., Muraleedharan N. (2004): Agrobacterium tumefaciens-Mediated genetic transformation in tea (Camellia sinensis [L.] O. Kuntze). Plant Molecular Biology Reporter, 22, 201-202
Mahmoudian Mehrzad, Yücel Meral, Öktem Hüseyin Avni (2002): Transformation of lentil (Lens culinaris M.) cotyledonary nodes by vacuum infiltration ofAgrobacterium tumefaciens. Plant Molecular Biology Reporter, 20, 251-257
Murashige Toshio, Skoog Folke (1962): A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497
Negishi Y., Hashimoto A., Tsushima M., Dobrota C., Yamashita M., Nakamura T. (1999): Growth of pea epicotyl in low magnetic field implication for space research. Advances in Space Research, 23, 2029-2032
Nester E.W., Amasino R., Akiyoshi D., Klee H., Montoya A., Gordon M.P. (1984): The molecular basis of plant cell transformation by Agrobacterium tumefaciens. Basic Life Science, 30: 815–822.
Okubo Hiroshi, Wada Koichi, Uemoto Shunpei (1991): In vitro morphogenetic response and distribution of endogenous plant hormones in hypocotyl segments of snapdragon (Antirrhinum majus L.). Plant Cell Reports, 10, -
Podlesny J., Misiak L., Podlesna A. (2004): Concentration of free radicals in pea seeds after pre-sowing treatment with magnetic field. International Agrophysics, 18: 261–267.
Reina Francisco Garc�a, Pascual Luis Arza (2001): Influence of a stationary magnetic field on water relations in lettuce seeds. Part I: Theoretical considerations. Bioelectromagnetics, 22, 589-595
Savostin P.W. (1930): Magnetic growth relations in plant. Planta, 12: 327.
Simkó Myrtill (2004): Induction of Cell Activation Processes by Low Frequency Electromagnetic Fields. The Scientific World JOURNAL, 4, 4-22
Snedecor G.W., Cochran W.G. (1989): Statistical Methods. 8th Ed., Ames, Iowa State University Press: 217–235.
Soltani F., Kashi A., Arghavani M. (2006): Effect of magnetic field on Asparagus officinalis L. seed germination and seedling growth. Seed Science and Technology, 34, 349-353
Spokevicius Antanas V., Van Beveren Kim, Leitch Mathew A., Bossinger Gerd (2005): Agrobacterium-mediated in vitro transformation of wood-producing stem segments in eucalypts. Plant Cell Reports, 23, 617-624
Welbaum G. E., Bradford K. J., Yim Kyu-Ock, Booth D. T., Oluoch M. O. (1998): Biophysical, physiological and biochemical processes regulating seed germination. Seed Science Research, 8, -
Yang L., Wang C., Wang L., Xu C., Chen K. (2013): An efficient multiplex PCR assay for early detection of Agrobacterium tumefaciens in transgenic plant material. Turkish Journal of Agriculture and Forestry, 37: 157–162.
YAO Y, LI Y, YANG Y, LI C (2005): Effect of seed pretreatment by magnetic field on the sensitivity of cucumber (Cucumis sativus) seedlings to ultraviolet-B radiation. Environmental and Experimental Botany, 54, 286-294
Yildiz Mustafa, Er Celâl (2002): The effect of sodium hypochlorite solutions on in vitro seedling growth and shoot regeneration of flax ( Linum usitatissimum ). Naturwissenschaften, 89, 259-261
Yildiz M., Özcan S., Er C. (2002): The effect of different explant sources on adventitious shoot regeneration in flax (Linum usitatissimum L.) Turkish Journal of Biology, 26: 37–40.
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