Molecular mapping of leaf rust resistance gene LrL224 in Chinese wheat cultivar L224-3 Y., Li H., Zhang P., Wang L., Li Z. (2018): Molecular mapping of leaf rust resistance gene LrL224 in Chinese wheat cultivar L224-3. Czech J. Genet. Plant Breed., 54: 65-70.
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Leaf rust, caused by Puccinia triticina, is a major wheat disease worldwide. The chinese wheat cultivar L224-3 showed high resistance to most of P. triticina pathotypes in the seedling and adult stage. Identifying and mapping the leaf rust resistance gene(s) in L224-3 is very useful for breeding leaf rust resistant wheat cultivars. In the present study, the wheat cultivar L224-3 and thirty-six lines with known leaf rust resistance genes were inoculated with 15 pathotypes at the seedling stage for gene postulation. A total of 144 F2:3 lines from the cross L224-3 × Zhengzhou 5389 were inoculated with the pathotype FHBQ for leaf rust genetic analysis at the seedling stage. A total of 1276 SSR (simple sequence repeat) markers and the STS (sequence tagged-site) marker ω-secali/Glu-B3 were used to test the parents, resistant and susceptible bulks. The polymorphic markers were used to genotype the F2:3 populations. L224-3 was highly resistant to all Lr26 avirulent pathotypes, showing the presence of Lr26 in L224-3. The presence of Lr26 in L224-3 was also confirmed by the molecular marker ω-secalin/Glu-B3. Due to resistance to some Lr26 virulent pathotypes, L224-3 may carry another resistance gene. Based on the genetic analysis using the pathotype FHBQ with virulence to Lr26 the resistance of L224-3 was controlled by a single dominant gene, tentatively designated LrL224. Four SSR markers (barc8, gwm582, wmc419, and wmc694) and one STS marker (ω-secali/Glu-B3) on 1B were closely linked to LrL224. The two flanking SSR loci were barc8 and gwm582, with the genetic distances of 4.3 and 4.6 cM, respectively. LrL224 was located on 1BL, and it showed different seedling reactions with other genes on 1B. Therefore LrL224 is likely to be a new leaf rust resistance gene.

Bassam Brant J., Caetano-Anollés Gustavo, Gresshoff Peter M. (1991): Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry, 196, 80-83
Bryan G. J., Collins A. J., Stephenson P., Orry A., Smith J. B., Gale M. D. (1997): Isolation and characterisation of microsatellites from hexaploid bread wheat. TAG Theoretical and Applied Genetics, 94, 557-563
Chai J. F., Zhou R. H., Jia J. Z., Liu X. (2006): Development and application of a new codominant PCR marker for detecting 1BL.1RS wheat-rye chromosome translocations. Plant Breeding, 125, 302-304
Chen X.C., Li X., Li Z.F., Zhang H., Chen H., Gao M., Liu D.Q. (2010): Molecular mapping of leaf rust resistance gene in Chinese wheat line Guizhou98-18. Acta Phytopathologica Sinica, 40: 489–494.
de Froidmont D. (1998): A Co-dominant Marker for the 1BL/1RS Wheat-rye Translocation via Multiplex PCR. Journal of Cereal Science, 27, 229-232
Dong J.G. (ed) (2001): Agricultural Plant Pathology. Beijing, China Agriculture Press.
P. Gupta, H. Balyan, K. Edwards, P. Isaac, V. Korzun, M. R�der, M.-F. Gautier, P. Joudrier, A. Schlatter, J. Dubcovsky, Pena R. De la, M. Khairallah, G. Penner, M. Hayden, P. Sharp, B. Keller, R. Wang, J. Hardouin, P. Jack, P. Leroy (2002): Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. TAG Theoretical and Applied Genetics, 105, 413-422
Hiebert Colin, Thomas Julian, McCallum Brent (2005): Locating the broad-spectrum wheat leaf rust resistance gene Lr52 (LrW) to chromosome 5B by a new cytogenetic method. Theoretical and Applied Genetics, 110, 1453-1457
Li X., Li Z.F., Li Y.N., Zhao Z.Q., Liu D.Q., Wang C.F., Gao L.J. (2010): Genetic analysis and molecular mapping of leaf rust resistance gene in wheat line Xinong 1163-4. Scientia Agriculturae Sinica, 43: 2397–2402.
Li Zaifeng, Lan Caixia, He Zhonghu, Singh Ravi P., Rosewarne Garry M., Chen Xinmin, Xia Xianchun (2014): Overview and Application of QTL for Adult Plant Resistance to Leaf Rust and Powdery Mildew in Wheat. Crop Science, 54, 1907-
Long D. L. (1989): A North American System of Nomenclature for Puccinia recondita f. sp. tritici. Phytopathology, 79, 525-
Manly Kenneth F., Cudmore, Robert H., Meer Jane M. (2001): Map Manager QTX, cross-platform software for genetic mapping. Mammalian Genome, 12, 930-932
McIntosh R.A., Devos K.M., Dubcovsky J., Rogers W.J., Morris C.F., Appels R., Anderson O.D. (2005): Catalogue of Gene Symbols for Wheat: 2005 Supplement. Available at
McIntosh R.A., Dubcovsky J., Rogers W.J., Morris C., Appels R., Xia X.C. (2016): Catalogue of Gene Symbols for Wheat: 2015−2016 Supplement. Available at
Michelmore R. W., Paran I., Kesseli R. V. (1991): Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations.. Proceedings of the National Academy of Sciences, 88, 9828-9832
Röder M.S., Korzun V., Wendehake K., Plaschke J., Tixier M.H., Leroy P., Ganal M.W. (1998): A microsatellite map of wheat. Genetics, 149: 2007–2023.
Roelfs A.P., Singh R.P., Saari E.E. (1992): Rust Diseases of Wheat: Concepts and Methods of Disease Management. Mexico, CIMMYT.
Rosewarne G. M., Singh R. P., Huerta-Espino J., William H. M., Bouchet S., Cloutier S., McFadden H., Lagudah E. S. (2006): Leaf tip necrosis, molecular markers and β1-proteasome subunits associated with the slow rusting resistance genes Lr46/Yr29. Theoretical and Applied Genetics, 112, 500-508
Sharp P. J., Kreis M., Shewry P. R., Gale M. D. (1988): Location of ?-amylase sequences in wheat and its relatives. Theoretical and Applied Genetics, 75, 286-290
Singh R. P., Mujeeb-Kazi A., Huerta-Espino J. (1998): Lr46 : A Gene Conferring Slow-Rusting Resistance to Leaf Rust in Wheat. Phytopathology, 88, 890-894
Somers Daryl J., Isaac Peter, Edwards Keith (2004): A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 109, 1105-1114
Song Q. J., Fickus E. W., Cregan P. B. (2002): Characterization of trinucleotide SSR motifs in wheat. TAG Theoretical and Applied Genetics, 104, 286-293
Sourdille Pierre, Singh Sukhwinder, Cadalen Thierry, Brown-Guedira Gina L., Gay Georges, Qi Lili, Gill Bikram S., Dufour Philippe, Murigneux Alain, Bernard Michel (2004): Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat ( Triticum aestivum L.). Functional & Integrative Genomics, 4, 12-25
Wang Cuifen, Yin Guihong, Xia Xianchun, He Zhonghu, Zhang Peipei, Yao Zhanjun, Qin Jinyan, Li Zaifeng, Liu Daqun (2016): Molecular mapping of a new temperature-sensitive gene LrZH22 for leaf rust resistance in Chinese wheat cultivar Zhoumai 22. Molecular Breeding, 36, -
Xing Lifang, Wang Cuifen, Xia Xianchun, He Zhonghu, Chen Wanquan, Liu Taiguo, Li Zaifeng, Liu Daqun (2014): Molecular mapping of leaf rust resistance gene LrFun in Romanian wheat line Fundulea 900. Molecular Breeding, 33, 931-937
Zhang Hai, Xia Xianchun, He Zhonghu, Li Xing, Li Zaifeng, Liu Daqun (2011): Molecular mapping of leaf rust resistance gene LrBi16 in Chinese wheat cultivar Bimai 16. Molecular Breeding, 28, 527-534
Zhao X. L., Zheng T. C., Xia X. C., He Z. H., Liu D. Q., Yang W. X., Yin G. H., Li Z. F. (2008): Molecular mapping of leaf rust resistance gene LrZH84 in Chinese wheat line Zhou 8425B. Theoretical and Applied Genetics, 117, 1069-1075
Zhou H.X., Xia X.C., He Z.H., Li X., Wang C.F., Li Z.F., Liu D.Q. (2013a): Molecular mapping of leaf rust resistance gene LrNJ97 in Chinese wheat line Neijiang 977671. Theoretical and Applied Genetics, 126: 2141–2147.
Zhou Y., Xia X.C., He Z.H. Li X., Li Z.F., Liu D.Q. (2013b): Fine mapping of leaf rust resistance gene LrZH84 using expressed sequence tag and sequence-tagged site markers, and allelism with other genes on wheat chromosome 1B. Phytopathology, 103: 169–174.
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