Variation in ploidy level and morphological traits in the progeny of the triploid apple variety Jonagold
Variation with respect to both ploidy level and morphology was characterized for a set of 690 seedlings of the triploid apple variety Jonagold, of which 481 were obtained via in vitro culture of mature embryos, and 209 via conventional germination. Their ploidy level was determined by a combination of flow cytometry and root tip chromosome counting. The assessed morphological traits were leaf length, width and shape, stomatal density, guard cell length and chloroplast width and number. A total of 452 seedlings were aneuploid, 225 diploid, nine triploid and four tetraploid. All four tetraploid seedlings were derived by in vitro culture. When the triploid seedlings were genotyped at the S-locus and at selected microsatellite loci, we found that the Jonagold stigma was compatible with pollen which shared some of the maternal parent S-locus alleles.
Bassam Brant J., Caetano-Anollés Gustavo, Gresshoff Peter M. (1991): Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry, 196, 80-83 https://doi.org/10.1016/0003-2697(91)90120-I
Bingham E. T. (1968): Stomatal Chloroplasts in Alfalfa at Four Ploidy Levels1. Crop Science, 8, 509- https://doi.org/10.2135/cropsci1968.0011183X000800040036x
Einset J. (1952): Spontaneous polyploidy in cultivated apples. Proceedings of the American Society for Horticaltural Science, 59: 291–302.
Jackson Scott, Chen Z Jeffrey (2010): Genomic and expression plasticity of polyploidy. Current Opinion in Plant Biology, 13, 153-159 https://doi.org/10.1016/j.pbi.2009.11.004
Jiao Yuannian, Wickett Norman J., Ayyampalayam Saravanaraj, Chanderbali André S., Landherr Lena, Ralph Paula E., Tomsho Lynn P., Hu Yi, Liang Haiying, Soltis Pamela S., Soltis Douglas E., Clifton Sandra W., Schlarbaum Scott E., Schuster Stephan C., Ma Hong, Leebens-Mack Jim, dePamphilis Claude W. (2011): Ancestral polyploidy in seed plants and angiosperms. Nature, 473, 97-100 https://doi.org/10.1038/nature09916
Koller B., Tenzer I., Gessler C. (2000): SSR analysis of apple scab lesions. Integrated Control of Pome Fruit Diseases. IOBC/WPRS Bulletin, 23: 93–98.
KONDO KATSUHIKO, LAVARACK PETER S. (1984): A cytotaxonomic study of some Australian species of Drosera L. (Droseraceae). Botanical Journal of the Linnean Society, 88, 317-333 https://doi.org/10.1111/j.1095-8339.1984.tb01579.x
Laubscher F.X., Hurter N. (1960): The chromosome numbers of seedling progeny from triploid apples. South African Journal of Science, 3: 31–39.
LESPINASSE Y., ALSTON F. H., WATKINS R. (1976): Cytological techniques for use in apple breeding. Annals of Applied Biology, 82, 349-353 https://doi.org/10.1111/j.1744-7348.1976.tb00570.x
Levin D. (2002): The Role of Chromosomal Change in Plant Evolution. New York, Oxford University Press.
Michaelson Martin J., Price H. James, Johnston J. Spencer, Ellison John R. (1991): VARIATION OF NUCLEAR DNA CONTENT IN HELIANTHUS ANNUUS (ASTERACEAE). American Journal of Botany, 78, 1238-1243 https://doi.org/10.1002/j.1537-2197.1991.tb11416.x
Minamikawa Mai F., Koyano Ruriko, Kikuchi Shinji, Koba Takato, Sassa Hidenori, Quint Marcel (2014): Identification of SFBB-Containing Canonical and Noncanonical SCF Complexes in Pollen of Apple (Malus × domestica). PLoS ONE, 9, e97642- https://doi.org/10.1371/journal.pone.0097642
Mochizuki A., Sueoka N. (1955): Genetic Studies on the Number of Plastid in Stomata. CYTOLOGIA, 20, 358-366 https://doi.org/10.1508/cytologia.20.358
Murashige T., Skoog F. (1962:) A revised medium for rapid growth and bioassays wih tobacco tissue cultures. Plant Physiology, 15: 473–497.
Najčevska C. M., Speckmann G. J. (1968): Numbers of chloroplasts and pollen grain pores in diploid and tetraploid varieties of some Trifolium species. Euphytica, 17, 357-362 https://doi.org/10.1007/BF00056236
Passvetaeva E.G. (1985): Identification of stone fruit cultivars and their hybrids that forming unreduced pollen. Plant Breeding Abstracts, 55: 8910–8912.
Rensing Stefan A (2014): Gene duplication as a driver of plant morphogenetic evolution. Current Opinion in Plant Biology, 17, 43-48 https://doi.org/10.1016/j.pbi.2013.11.002
Riccardi Carlo, Nicoletti Ildo (2006): Analysis of apoptosis by propidium iodide staining and flow cytometry. Nature Protocols, 1, 1458-1461 https://doi.org/10.1038/nprot.2006.238
Sanford J.C. (1983): Ploidy manipulation. In: Methods in Fruit Breeding. West Lafayette, Purdue University Press: 100–123.
Soltis Douglas E., Soltis Pamela S., Tate Jennifer A. (2004): Advances in the study of polyploidy since Plant speciation. New Phytologist, 161, 173-191 https://doi.org/10.1046/j.1469-8137.2003.00948.x
Soltis Pamela S, Marchant D Blaine, Van de Peer Yves, Soltis Douglas E (2015): Polyploidy and genome evolution in plants. Current Opinion in Genetics & Development, 35, 119-125 https://doi.org/10.1016/j.gde.2015.11.003