Mapping of quantitative trait loci for purple stigma and purple apiculus in rice by using a Zhenshan 97B/Minghui 63 RIL population

Tong J.P., Han Z.-S., Han A.N. (2021): Mapping of quantitative trait loci for purple stigma and purple apiculus in rice by using a Zhenshan 97B/Minghui 63 RIL population. Czech J. Genet. Plant Breed., 57: 113118.

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Anthocyanin pigmentation is an important morphological marker that is commonly used to identify rice varieties and for linkage analysis. The following study investigates the genetic factors involved in the purple stigma (Ps) and purple apiculus (Pa) traits of an important indica rice cross between Zhenshan 97 (purple stigma and purple apiculus) and Minghui 63 (grey stigma and colourless apiculus). A recombinant inbred line (RIL) population derived from this cross was used for quantitative trait loci (QTL) mapping of the purple stigma and purple apiculus traits. As a result, one major QTL for the purple stigma trait, temporarily designated qPS-1-1, and one major QTL for the purple apiculus trait, temporarily designated qPA-1-1, were mapped to the short arm of chromosome 6 in the interval between the two markers Y4073L and *P. The LOD peaks of qPS-1-1 and qPA-1-1 were 44.0127 and 173.3585, respectively. In addition, qPS-1-1 and qPA-1-1 explained 66.7416% and 98.6441% of the total phenotypic variance, respectively. The Zhenshan 97 allele increased the purple stigma trait by approximately 8.0355% (for qPS-1-1) and 9.8863% (for qPA-1-1). Moreover, since qPS-1-1 and qPA-1-1 were strongly correlated, they were also located in the same vicinity of the C gene on the short arm of chromosome 6, which suggested that the two QTL might be the same. By comparing these and previous results, it was deduced that qPS-1-1 or qPA-1-1 was the C gene and was pleiotropic for both the colouration of the apiculus and the colouration of the stigma in rice.

Chandler V.L., Radicella J.P., RobbinsT.P., Chen J., Turks D. (1989): Two regulatory genes of the maize anthocyanin pathway are homologous: Isolation of B utilizing R genomic sequences. Plant Cell, 1: 1175–1183.
Collard B.C.Y., JahuferM Z.Z., Brouwer J.B., Pang E.C.K. (2005): An introduction to markers, quantitative trait loci (QTL) mapping and marker-assisted selection for crop improvement: The basic concepts. Euphytica, 142: 169–196.
Dooner H.K., Robbins T.P., Jorgensen R.A. (1991): Genetic and developmental control of anthocyanin biosynthesis. Annual Review of Genetics, 25: 173–199.
Fan F.J., Fan Y.Y., Du J.H., Zhuang J.Y. (2008): Fine mapping of C (Chromogen for anthocyanin) gene in rice. Rice Science, 15: 1–6.
Han L., Zhang T., Xu J., Di., Li Y., Wang X.D., Wu X.J. (2006): Genetic analysis and gene mapping of purple stigma in rice. Acta Genetica Sinica, 33: 642–646.
Hu J., Anderson B., Wessler R. (1996): Isolation and characterization of rice R genes: Evidence for distinct evolutionary paths in rice and maize. Genetics, 142: 1021–1031.
Khlestkina E.K. (2013): Genes determining coloration of different organs in wheat. Russian Journal of Genetics Applied Research, 3: 54–65.
Kinoshita T. (1984): Current linkage maps. Rice Genetics Newsletter, 1: 16–27.
Kishimoto N., Shimosaka E., Matsuura S., Saito A. (1992): A current RFLP linkage map of rice: Alignment of the molecular map with the classical map. Rice Genetics Newsletter, 9: 118–124.
Koes R.E., Quattrocchio F., Mol J.N.M. (1994): The flavonoid biosynthetic pathway in plants: Function and evolution. BioEssays, 16: 123–132.
Li W.C., Wang J.W., Yu J.J. (2012): Genetic analysis and molecular mapping of a purple-stigma mutant (ps-5) in rice. Journal of Henan Normal University (Natural Science Edition), 40: 121–124. (in Chinese with English abstract)
Li W.L., Faris J.D., Chittoor J.M., Leach J.E., Hulbert S.H., Liu D.J., Chen P.D., Gill B.S. (1999): Genomic mapping of defense response genes in wheat. Theoretical and Applied Genetics, 98: 226–233.
Li Z., Pinson S.R., Stansel J.W., Park W.D. (1995): Identification of quantitative trait loci (QTLs) for heading date and plant height in cultivated rice (Oryza sativa L.). Theoretical and Applied Genetics, 91: 374–381.
Liu K.D., Wang J., Li H.B., Xu C.G., Liu A.M., Li X.H., Zhang Q.F. (1997): A genome-wide analysis of wide compatibility in rice and the precise location of the S5 locus on the molecular map. Theoretical and Applied Genetics, 95: 809–814.
Liu X., Sun X., Wang W.Y., Ding H.F., Liu W., Li G.X., Jiang M.S., Zhu C.X., Yao F.Y. (2012): Fine mapping of Pa-6 gene for purple apiculus in rice. Journal of Plant Biology, 55: 218–225.
Ludwig S.R., Habera L.F., Dellaporta S.L., Wessler S.R. (1989): Lc, a member of the maize R gene family responsible for tissue-specific anthocyanin production, encodes a protein similar to transcription activators and contains the myc-homology region. Proceedings of the National Academy of Science USA, 86: 7092–7096.
Mackill D.J., Nguyen H.T., Zhan J. (1999): Use of molecular markers in plant improvement programs for rainfed lowland rice. Field Crops Research, 64: 177–185.
Maekawa M., Kita F. (1987): Genic analysis for weakness induced with gamma-ray treatment and mechanisms for the occurrence of triploid plants of rice. Japan Journal of Breeding, 37 (Suppl. 2): 308–309. (in Japanese with English abstract)
Martin C., Paz-Ares J. (1997): MYB transcription factors in plants. Trends in Genetics, 13: 67–73.
McCouch S.R., Doerge R.W. (1995): QTL mapping in rice. Trends in Genetics, 11: 482–487.
McCouch S.R., Kochert G., Yu ZH., Wang Z.Y., Khush G.S., Coffman W.R., Tanksley S.D. (1988): Molecular mapping of rice chromosomes. Theoretical and Applied Genetics, 76: 815–829.
Meng L., Li H., Zhang L., Wang J. (2015): QTL IciMapping: Integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations. Crop Journal, 3: 269–283.
Mikami I., Takahashi A., Khin-thidar A., Sano Y. (2000): A candidate for C (Chromogen for anthocyanin) gene. Rice Genetics Newsletter, 17: 54–56.
Mohan M., Nair S., Bhagwat A., Krishna T.G., Yano M., Bhatia C.R., Sasaki T. (1997): Genome mapping, molecular markers and marker-assisted selection in crop plants. Molecular Breeding, 3: 87–103.
Nagao S., Takahashi M. (1963): Trial construction of twelve linkage groups in Japanese rice (Genetical studies of rice plant, XXVII). Journal of the Faculty of Agriculture, Hokkaido University, 53: 72–130.
Paterson A.H. (1996a): Making genetic maps. In: Paterson A.H. (ed.): Genome Mapping in Plants. Austin, Academic Press: 23–39.
Paterson A.H. (1996b): Mapping genes responsible for differences in phenotype. In: Paterson A.H. (ed.): Genome Mapping in Plants. Austin, Academic Press: 41–54.
Paz-Ares J., Ghosal D., Wienand U., Petersont P.A., Saed-ler H. (1987): The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators. EMBO Journal, 6: 3553–3558.
Reddy A.R. (1996): Genetic and molecular analysis of the anthocyanin pigmentation pathway in rice. In: Khush G.S., Hettel G., Rola T. (eds.): Rice Genetics III, Manila, IRRI: 341–352.
Reddy A.R., Scheffler B., Madhuri G., Srivastava M.N., Kumar A., Sathyanarayanan P.V., Nair S., Mohan M. (1996): Chalcone synthase in rice (Oryza sativa L.): Detection of the CHS protein in seedlings and molecular mapping of the chs locus. Plant Molecular Biology, 32: 735–743.
Saitoh K., Onishi K., Mikami I., Thidar K., Sano Y. (2004): Allelic diversification at the C(OsC1) locus of wild and cultivated rice: Nucleotide changes associated with phenotypes. Genetics, 168: 997–1007.
Shoeva O.Y., Gordeeva E.I., Khlestkina E.K. (2014): The regulation of anthocyanin synthesis in the wheat pericarp. Molecules, 19: 20266–20279.
Takahashi M. (1957): Analysis on apiculus color genes essential to anthocyanin coloration in rice. Journal of the Faculty of Agriculture, Hokkaido University, 50: 266–362.
Takahashi M. (1982): Gene analysis and its related problems – Genetical studies on rice plant. Journal of the Faculty of Agriculture, Hokkaido University, 61: 91–142.
Tereshchenko O.Y., Arbuzova V.S., Khlestkina E.K. (2013): Allelic state of the genes conferring purple pigmentation in different wheat organs predetermines transcriptional activity of the anthocyanin biosynthesis structural genes. Journal of Cereal Science, 57: 10–13.
Wang C., Shu Q. (2007): Fine mapping and candidate gene analysis of purple pericarp gene Pb in rice (Oryza sativa L.). Chinese Science Bulletin, 52: 3097–3104.
Wang J., Liu K.D., Xu C.G., Li X.H., Zhang Q.F. (1998): The high level of wide-compatibility of variety “Dular” has a complex genetic basis. Theoretical and Applied Genetics, 97: 407–412.
Wu K.S., Tanksley S.D. (1993): Abundance, polymorphism and genetic mapping of microsatellites in rice. Molecular and General Genetics, 241: 225–235.
Xing Y.Z., Tan Y.F., Hua J.P., Sun X., Xu C.G., Zhang Q.F. (2002): Characterization of the main effects, epistatic effects and their environmental interactions of QTLs in the genetic basis of yield traits in rice. Theoretical and Applied Genetics, 105: 248–257.
Yue B., Cui K.H., Yu Sh.B., Xue W.Y., Luo L.J., Xing Y.Z. (2006): Molecular marker-assisted dissection of quantitative trait loci for seven morphological traits in rice (Oryza Sativa L.). Euphytica, 50: 131–139.
Zhao L.F. (1928): Linkage studies in rice. Genetics, 13: 133–169.
Zhao S., Wang C., Ma J., Wang S., Tian P., Wang J., Cheng Z., Zhang X., Guo X., Lei C. (2016): Map-based cloning and functional analysis of the chromogen gene C in rice (Oryza sativa L.). Journal of Plant Biology, 59: 496–505.
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