Stable inheritance of excellent agricultural traits induced by 12C6+ heavy-ions in lentil (Lens culinaris Medik.) W., Wu Z., Zhang Y., Wu D., Liu D., Wang Y., Gao Q., Dang B., Li W., Hou S. (2015): Stable inheritance of excellent agricultural traits induced by 12C6+ heavy-ions in lentil (Lens culinaris Medik.). Czech J. Genet. Plant Breed., 51: 29-35.
download PDF
The lentil (Lens culinaris Medik.) belongs to the most important leguminous plants in Asia. It is a very popular and highly nutritious food. However, small pod size and low yield limit its agricultural use. Through irradiation mutagenesis of dry lentil seeds by 12C6+ ion beam, we found some lines with excellent agricultural traits in M2 progeny at doses of 90 Gy (grey), such as height increase and improvement of the number of branches and three-seed pods as well as seeds with increased grain yield. Six generations of irradiated lentils were screened for these traits. In the M8 generation, the grain yield of three high-yielding lentil lines (lines hyl-1, hyl-2, and hyl-3) reached 212.4%, 195.3% and 190.8%, respectively, of the non-irradiated controls. The results indicated that crop improvement was stable inherited across the generations. Statistical analysis revealed that the increase in grain yield was due to increased total pod number and seeds per pod. Fortunately, adverse effects of irradiation (e.g. reduced germination rates and poor pollen vitality) disappeared over the eight generations. In conclusion, we present a practical method for the improvement of lentils through radiation breeding, leading to high yielding cultivars, which could support the use of this crop in agriculture.
ABE TOMOKO, MATSUYAMA TOMOKI, SEKIDO SHIGEKO, YAMAGUCHI ISAMU, YOSHIDA SHIGEO, KAMEYA TOSHIAKI (2002): Chlorophyll-deficient Mutants of Rice Demonstrated the Deletion of a DNA Fragment by Heavy-ion Irradiation. Journal of Radiation Research, 43, S157-S161
Cui H.B. (1998): Flora of China 42. Beijing, Science Press. (in Chinese)
Fedoruk Michael J., Vandenberg Albert, Bett Kirstin E. (2013): Quantitative Trait Loci Analysis of Seed Quality Characteristics in Lentil using Single Nucleotide Polymorphism Markers. The Plant Genome, 6, 0-
FENG H, YU Z, CHU P (2006): Ion implantation of organisms. Materials Science and Engineering: R: Reports, 54, 49-120
Huang Xianzhong, Qian Qian, Liu Zhengbin, Sun Hongying, He Shuyuan, Luo Da, Xia Guangmin, Chu Chengcai, Li Jiayang, Fu Xiangdong (): Natural variation at the DEP1 locus enhances grain yield in rice. Nature Genetics, 41, 494-497
Mondal M.M.A., Puteh A.B., Malek M.A., Roy S., Yusop M.R. (2013): Contribution of morpho-physiological traits on yield of lentil (Lens culinaris Medik L.). Australian Journal of Crop Science, 7: 1167–1172.
Podleśny J. (2002): Effect of laser irradiation on biochemical changes in seeds and accumulation of dry matter in the faba bean. International Agrophysics, 16: 209–213.
Roychowdhury R., Tah J. (2013): Mutagenesis – A potential approach for crop improvement. In: Hakeem K.R. (ed.): Crop Improvement: New Approaches and Modern Techniques. New York, Springer-Verlag, 149–187.
Shikazono N., Tanaka A., Watanabe H., Tano S. (2001): Rearrangements of the DNA in carbon ion-induced mutants of Arabidopsis thaliana. Genetics, 157: 379–387.
Shikazono N. (2005): Analysis of mutations induced by carbon ions in Arabidopsis thaliana. Journal of Experimental Botany, 56, 587-596
Suvorova G. (2014): Hybridization of cultivated lentil Lens culinaris Medik. and wild species Lens tomentosus Ladi-zinsky. Czech Journal of Genetics and Plant Breeding, 50: 130–134.
TANAKA Atsushi, SHIKAZONO Naoya, HASE Yoshihiro (2010): Studies on Biological Effects of Ion Beams on Lethality, Molecular Nature of Mutation, Mutation Rate, and Spectrum of Mutation Phenotype for Mutation Breeding in Higher Plants. Journal of Radiation Research, 51, 223-233
WADA SEIICHI, KOBAYASHI YASUHIKO, FUNAYAMA TOMOO, NATSUHORI MASAHIRO, ITO NOBUHIKO, YAMAMOTO KAZUO (2002): Detection of DNA Damage in Individual Cells Induced by Heavy-ion Irradiation with an Non-denaturing Comet Assay. Journal of Radiation Research, 43, S153-S156
Wu D.L., Hou S.W., Qian P.P., Sun L.D., Zhang Y.C., Li W.J. (2009): Flower color chimera and abnormal leaf mutants induced by 12C6+ heavy ions in Salvia splendens Ker-Gawl.. Scientia Horticulturae, 121, 462-467
Younis N., Hanif M., Sadiq S., Abbas G., Asghar M.J., Haq M.A. (2008): Estimation of genetic parameters and path analysis in lentil. Pakistan Journal of Agricultural Sciences, 45: 44–48.
download PDF

© 2020 Czech Academy of Agricultural Sciences