The sulphonylurea herbicide monosulphuron ester sodium as a special male gametocide in Brassica napus L.

https://doi.org/10.17221/233/2014-CJGPBCitation:Cheng Y.-., Cui J.-., Li Z.-., Hu Z.-., Xing Z.-., Wang J., Zhao H.-., Hu S.-. (2015): The sulphonylurea herbicide monosulphuron ester sodium as a special male gametocide in Brassica napus L. Czech J. Genet. Plant Breed., 51: 16-22.
download PDF
The sulphonylurea herbicide monosulphuron ester sodium (MES) was first used to induce male sterility in rapeseed (Brassica napus L.). Nearly 100% male sterility was achieved after a single treatment with 10 ml of 0.05–0.10 μg/ml MES solution per plant to the cultivars Zhongshuang No.9 and Zhongshuang No.11 at the uninucleate stage of the longest buds. No adverse effects on main agronomic traits and female fertility were observed. In the subsequent experiment, 48 out of 49 different accessions showed male sterility after spraying with 10 ml of 0.10 μg/ml MES solution per plant. Seven out of these 48 accessions were male sterile for the entire flowering period. Seed number per plant of these MES-treated plants was not significantly reduced compared to the controls. The hybridity was 95.5% in the case of seeds harvested from the ZS09 inbred line if sprayed twice with 10 ml of 0.10 µg/ml MES solution per plant and pollinated by the SH11 male line. These results suggest that MES is an efficient chemical hybridising agent for B. napus.
References:
Bus Anja, Körber Niklas, Snowdon Rod J., Stich Benjamin (2011): Patterns of molecular variation in a species-wide germplasm set of Brassica napus. Theoretical and Applied Genetics, 123, 1413-1423  https://doi.org/10.1007/s00122-011-1676-7
 
Chen X.J., Qin C.Q., Zhang J.F., Pu H.M., Gao J.Q., Fu S.Z. (2002): Application of chemical gametocide No. 2 to B. napus L. Jiangsu Agricultural Sciences, 6: 19–21. (in Chinese)
 
Guan C.Y. (1995): Chemical induced male sterility and other ways of heterosis breeding. In: Fu T.D. (ed.): Breeding and Utilization of Hybrid Rapeseed. Wuhan, Hubei Science and Technology Press: 124–133. (in Chinese)
 
Guan C.Y. (2012): New Technologies of Rapeseed Heterosis Utilization: the Use of Chemical Hybridization Agent. Beijing, Science Press. (in Chinese)
 
Guan C.Y., Stringam G.R. (1998): The effect of ZMA on inducing male sterility on spring canola. Cruciferae Newsletter, 20: 55–56.
 
Guan C.Y., Wang G.D., Zhao J.T., Li H.Z. (1981): A preliminary study on male-sterility of rapeseed induced by male gametocide No.1. Hereditas (Beijing), 3: 15–17. (in Chinese)
 
Guan C.Y., Li X., Wang G.H., Chen S.Y., Yuan Y.S. (1998): Studies of mechanism on male sterility in rape induced by chemical hybridization agent II. The effect of KMS-1 on fertility in rapeseed. Chinese Journal of Oil Crop Sciences, 20: 1–4. (in Chinese)
 
Jing M., Wang K., Wang B., Wei C., Dong Z.S., Li Y.X. (2008): Further study on male sterilizing effect of BHL on rapeseed. Acta Agriculturae Boreali-occidentalis Sinica, 18: 150–152. (in Chinese)
 
Kou J.J., Li Z.M., Song H.B. (2006): Synthesis and crystal structure of a sodium monosulfuron-ester (N-[2'-(4-methyl) pyrimidinyl]-2-carbomethoxy benzyl sulfonyl-urea sodium). Chinese Journal of Structural Chemistry, 25: 1414–1417.
 
Kou J.J., Ju G.D., Li Z.M. (2013): Synthesis and herbicidal activity of sodium monosulfuron-ester. Chinese Journal of Pesticide Science, 15: 356–358. (in Chinese)
 
Liu X.X., Dong J.G., Liu C.S., Dong Z.S., Yan Z.B., Gao X.L., Gao C.Y. (2007): Effect and utility of a novel chemical hybridizing agent EN on Brassica napus. Journal of Northwest A&F University (Nat. Sci. Ed.), 35: 81–85. (in Chinese)
 
Piquemal J., Cinquin E., Couton F., Rondeau C., Seignoret E., doucet I., Perret D., Villeger M.-J., Vincourt P., Blanchard P. (2005): Construction of an oilseed rape (Brassica napus L.) genetic map with SSR markers. Theoretical and Applied Genetics, 111, 1514-1523  https://doi.org/10.1007/s00122-005-0080-6
 
Singh V., Chauhan S. V. S. (2003): Bud pollination and hybrid seed production in detergent-induced male sterile plants of Brassica juncea. Plant Breeding, 122, 421-425  https://doi.org/10.1046/j.1439-0523.2003.00880.x
 
Song L.H., Zhi W.L., Li W., Hu S.W. (2011): Genetic diversity of Chinese cultivars in Brassica napus revealed by SSR markers. Journal of Northwest A&F University (Nat. Sci. Ed.), 39: 110–118. (in Chinese)
 
SPSS Inc. (2001): SPSS (Satistical Product and Service Solutions) 11.0 for windows. Available at http://www.spss.com/spss
 
Tang Q.Y., Feng M.G. (1997): Practical Statistics and DPS Data Processing System. Beijing, Chinese Agricultrul University Press. (in Chinese)
 
Wang M.Y., Kou J.J., Ju G.D., Liu B.S. (2008): Applied research of a new sulfonylurea herbicide monosulfuron. Agrochemicals, 47: 412–422.
 
Yan Z.B., Liu C.S., Dong J.G., Chen R.X., Liu X.X., Gao X.L. (2006): Effects of chemical hybridizing agent ESP on Brassica napus L. Acta Agriculturae Boreali-occidentalis Sinica, 15: 81–84. (in Chinese)
 
Yu C.Y., He P.R. (2014): Evaluation of male-sterility induction effect of various amino acid biosynthesis inhibiting-herbicides on rapeseed (Brassica napus). Acta Agronomica Sinica, 40: 264–272. (in Chinese)
 
Yu C.Y., Hu S.W., Zhang C.H., Yu Y.J., He P.R. (2005): The effect of chemical hybridizing agent EXP on oilseed rape. Acta Agronomica Sinica, 31: 1455–1459. (in Chinese)
 
Yu C., Hu S., He P., Sun G., Zhang C., Yu Y. (2006): Inducing male sterility in Brassica napus L. by a sulphonylurea herbicide, tribenuron-methyl. Plant Breeding, 125, 61-64  https://doi.org/10.1111/j.1439-0523.2006.01180.x
 
Yu C.-Y., Dong J.-G., Hu S.-W., He P.-R. (2009): Efficiency of a novel gametocide amidosulfuron on rapeseed ( Brassica napus ). Plant Breeding, 128, 538-540  https://doi.org/10.1111/j.1439-0523.2008.01594.x
 
Zhang X.K., Li J.N., Tang Z.L., Li C., Chen Y.P. (1999): Effects of a chemical hybridization agent on the fertility of Polima male sterile lines of rape (Brassica napus L.). Journal of Southwest Agricultural University, 21: 140–143. (in Chinese)
 
Zhang B.J., Zhao H.X., Hu S.W. (2010): Male sterile-inducing ability of tribenuron-methyl to rapeseed cultivar Zhongshuang 9. Chinese Journal of Oil Crop Sciences, 32: 467–471. (in Chinese)
 
download PDF

© 2020 Czech Academy of Agricultural Sciences