Lignin biosynthesis regulated by the antisense 4CL gene in alfalfa J., Li C., Zhao M., Wang C., Ru Y., Cui Z., Han Y.: (2018): Lignin biosynthesis regulated by the antisense 4CL gene in alfalfa. Czech J. Genet. Plant Breed., 54: 26-29.
download PDF

The Antisense 4CL gene was transfected into alfalfa through Agrobacterium-mediated transfer. The test results indicated that the antisense 4CL gene was successfully integrated into the genome DNA of alfalfa and was stably transmitted to the offspring. Compared to the wild-type plants, the lignin content of T0 and T1 generation plants was reduced by 45.77% and 31.97%, respectively; there were no significant differences in height and weight of T0 and T1 plants, compared to the wild-type plants. However, the transgenic plant differed from the wild-type plant by softer stems and leaves, larger leaves, fewer flowers and a fewer seeds. The T0 line was susceptible to disease infection, but significantly improved in the second year. The results suggest that the 4CL gene from Amorpha fruticosa can be used to regulate lignin biosynthesis in transgenic forage crops.

Baucher M., Bernard-Vailhe M.A., Chabbert B., Besle J.M., Opsomer C., Montagu M.V., Botterman J. (1999): Down-regulation of cinnamyl alcohol dehydrogenase in transgenic alfalfa (Medicago sativa L.) and the effect on lignin composition and digestibility. Plant Molecular Biology, 39: 437–447.
Holsters M., de Waele D., Depicker A., Messens E., van Montagu M., Schell J. (1978): Transfection and transformation of Agrobacterium tumefaciens. Molecular and General Genetics MGG, 163, 181-187
Hu Wen-Jing, Harding Scott A., Lung Jrhau, Popko Jacqueline L., Ralph John, Stokke Douglas D., Tsai Chung-Jui, Chiang Vincent L. (1999): Repression of lignin biosynthesis promotes cellulose accumulation and growth in transgenic trees. Nature Biotechnology, 17, 808-812
Van Huan Ha, Van Giang Ho, Van Thanh Ngo, Zhang Siliang, Wang Yong (2012): Identification and functional analysis of the Pm4CL1 gene in transgenic tobacco plant as the basis for regulating lignin biosynthesis in forest trees. Molecular Breeding, 29, 173-180
Kajita S., Katayama Y., Omori S. (1996): Alterations in the Biosynthesis of Lignin in Transgenic Plants with Chimeric Genes for 4-Coumarate: Coenzyme A Ligase. Plant and Cell Physiology, 37, 957-965
Lu Hai, Zeng Qingyin, Zhao YanLing, Wang Shasheng, Jiang Xiangning (2003): Xylem-specific expression of a GRP1.8 promoter::4CL gene construct in transgenic tobacco. Plant Growth Regulation, 41, 279-286
Murashige Toshio, Skoog Folke (1962): A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497
Song Y., Wu Y.C., Zhang Y., Wang Z.Z. (2011): Determination of lignin content and lignin monomer composition in Salvia miltiorrhiza Bge. Journal of Analytical Science, 27: 586–590.
Vanholme R., Demedts B., Morreel K., Ralph J., Boerjan W. (2010): Lignin Biosynthesis and Structure. PLANT PHYSIOLOGY, 153, 895-905
Voo K. S., Whetten R. W., O'Malley D. M., Sederoff R. R. (1995): 4-Coumarate:Coenzyme A Ligase from Loblolly Pine Xylem (Isolation, Characterization, and Complementary DNA Cloning). Plant Physiology, 108, 85-97
Yuan Zi-Qiang, Yu Kai-Liang, Epstein Howard, Fang Chao, Li Jun-Ting, Liu Qian-Qian, Liu Xue-Wei, Gao Wen-Juan, Li Feng-Min (2016): Effects of legume species introduction on vegetation and soil nutrient development on abandoned croplands in a semi-arid environment on the Loess Plateau, China. Science of The Total Environment, 541, 692-700
download PDF

© 2018 Czech Academy of Agricultural Sciences