Identification of a leaf rust resistance gene in the Chinese wheat line LB0288

https://doi.org/10.17221/2/2015-CJGPBCitation:Qi A., Li X., Shi L., Liu D., Li Z. (2015): Identification of a leaf rust resistance gene in the Chinese wheat line LB0288. Czech J. Genet. Plant Breed., 51: 43-49.
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Leaf rust (LR), caused by Puccinia triticina (Pt), is worldwide one of the most spread diseases in common wheat (Triticum aestivum L.). With the objective of identifying and mapping new genes for resistance to leaf rust, F1 and F2 plants from a cross between the resistant cultivar LB0288 and the susceptible cultivar Thatcher were inoculated with the Chinese P. triticina pathotype THTT in the greenhouse. A set of 1273 simple sequence repeat (SSR) primer pairs were used to test the parents and the resistant and susceptible bulks. The results indicated, that LB0288 carried a single dominant resistance gene LrLB88, closely linked to the CAPS marker of Lr1 (WR003) and the SSR marker Xbarc144, with genetic distances of 0 cM and 5.3 cM, respectively. Based on the chromosomal location and seedling reactions, it can be concluded that LrLB88 might be a new leaf rust resistance gene. These markers can be useful for marker assisted selection in breeding of leaf rust resistant wheat cultivars.
References:
Bassam B.J., Caetano-Anolles G., Gresshoff P.M. (1991): Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry, 196: 80–83.
 
Bryan G.J., Collins A.J., Stephenson P., Orry A., Smith J.B., Gale M.D. (1997): Isolation and characterization of microsatellites from hexaploid bread wheat. Theoretical and Applied Genetics, 94: 557–563.
 
Chen X.C., Li X., Li Z.F., Zhang H., Chen H., Gao M., Liu D.Q. (2010): Molecular mapping of leaf rust resistance gene in Chinese wheat line Guizhou98-18. Acta Phytopathologica Sinica, 40: 489–494.
 
Dong J.G. (2001): Agricultural Plant Pathology. Beijing, China Agriculture Press.
 
Dubin H. J. (1989): Postulated Genes for Resistance to Stripe Rust in Selected CIMMYT and Related Wheats. Plant Disease, 73, 472- https://doi.org/10.1094/PD-73-0472
 
Gupta P.K., Balyan H.S., Edwards K.J., Isaac P., Korzun V., Röder M., Gautier M.F., Joudrier P., Schlatter A.R., Dubcovsky J., De la Pena R.C., Khairallah M., Penner G., Hayden M.J., Sharp P., Keller B., Wang R.C.C., Hardouin J.P., Jack P., Leroy P. (2002): Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theoretical and Applied Genetics, 105: 413–422.
 
Järve K, Peusha H O, Tsymbalova J, Tamm S, Devos K M, Enno T M (2000): Chromosomal location of a Triticum timopheevii - derived powdery mildew resistance gene transferred to common wheat. Genome, 43, 377-381 https://doi.org/10.1139/g99-141
 
Knott D.R. (1989): The wheat rusts – breeding for resistance. Theoretical and Applied Genetics, 12: 162–181.
 
Kolmer J. A. (2003): Postulation of Leaf Rust Resistance Genes in Selected Soft Red Winter Wheats. Crop Science, 43, 1266- https://doi.org/10.2135/cropsci2003.1266
 
KOSAMBI D. D. (1943): THE ESTIMATION OF MAP DISTANCES FROM RECOMBINATION VALUES. Annals of Eugenics, 12, 172-175 https://doi.org/10.1111/j.1469-1809.1943.tb02321.x
 
Li X., Li Z.F., Li Y.N., Zhao Z.Q., Liu D.Q., Wang C.F., Gao L.J. (2010a): Genetic analysis and molecular mapping of leaf rust resistance gene in wheat line Xinong 1163-4. Scientia Agricultura Sinica, 43: 2397–2402.
 
Li Z.F., Xia X.C., He Z.H., Li X., Zhang L.J., Wang H.Y., Meng Q.F., Yang W.X., Li G.Q., Liu D.Q. (2010b): Seedling and slow rusting resistance to leaf rust in Chinese wheat cultivars. Plant Disease, 94: 45–53.
 
Liu Z.Y., Sun Q.X., Ni Z.F., Nevo E., Yang T. (2002): Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer. Euphytica, 123: 21–29.
 
Long D.L., Kolmer J.A. (1989): A North American system of nomenclature for Puccinia triticina. Phytopathology, 79: 525–529.
 
Manly Kenneth F., Cudmore, Robert H., Meer Jane M. (2001): Map Manager QTX, cross-platform software for genetic mapping. Mammalian Genome, 12, 930-932 https://doi.org/10.1007/s00335-001-1016-3
 
McIntosh R.A., Yamazaki Y., Dubcovsky J., Rogers W.J., Morris C., Appels R., Xia X.C. (2013): Catalogue of gene symbols for wheat: 2013 supplement. Available at http://www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/2013/GeneCatalogueIntroduction.pdf
 
Mebrate Sewalem A., Dehne Heinz-W., Pillen Klaus, Oerke Erich-C. (2008): Postulation of Seedling Leaf Rust Resistance Genes in Selected Ethiopian and German Bread Wheat Cultivars. Crop Science, 48, 507- https://doi.org/10.2135/cropsci2007.03.0173
 
Michelmore R.W., Paran I., Kesseli R.V. (1991): Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proceedings of the National Academy of Sciences of the United States of America, 88: 9828–9832.
 
Oelke L.M., Kolmer J.A. (2004): Characterization of leaf rust resistance in hard red spring wheat cultivars. Plant Disease, 88: 1127–1133.
 
Person C. (1959): Gene-for-gene relationships in host: Parasite systems. Canadian Journal of Botany – Revue Canadienne de Botanique, 37: 1101–1130.
 
Qiu J.W., Schürch A.C., Yahiaoui N., Dong L.L., Fan H.J., Zhang Z.J., Keller B., Ling H.Q. (2007): Physical mapping and identification of a candidate for the leaf rust resistance gene Lr1 of wheat. Theoretical and Applied Genetics, 115: 159–168.
 
Röder M.S., Korzun V., Wendehake K., Plaschke J., Tixier M.H., Leroy P., Ganal M.W. (1998): A microsatellite map of wheat. Genetics, 149: 2007–2023.
 
Roelfs A.P., Singh R.P., Saari E.E. (1992): Rust Diseases of Wheat: Concepts and Methods of Disease Management. Mexico, CIMMYT.
 
Samborski D.J. (1963): A mutation in Puccinia recondita Rob. ex Desm. f.sp. tritici to virulence on transfer, Chinese Spring × Aegilops umbellulata Zhuk. Canadian Journal of Botany – Revue Canadienne de Botanique, 41: 475–479.
 
Sharp P. J., Kreis M., Shewry P. R., Gale M. D. (1988): Location of ?-amylase sequences in wheat and its relatives. Theoretical and Applied Genetics, 75, 286-290 https://doi.org/10.1007/BF00303966
 
Song Q.J., Fickus E.W., Cregan P.B. (2002): Characterization of trinucleotide SSR motifs in wheat. Theoretical and Applied Genetics, 104: 286–293.
 
Sourdille P., Singh S., Cadalen T., Brown-Guedira G.L., Gay G., Qi L., Gill B.S., Dufour P., Murigneux A., Bernard M. (2004): Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Functional and Integrative Genomics, 4: 12–25.
 
Wamishe Yeshi A., Milus Eugene A. (2004): Genes for Adult-Plant Resistance to Leaf Rust in Soft Red Winter Wheat. Plant Disease, 88, 1107-1114 https://doi.org/10.1094/PDIS.2004.88.10.1107
 
Xing L.F., Wang C.F., Xia X.C., He Z.H., Chen W.Q., Liu T.G., Li Z.F., Liu D.Q. (2014): Molecular mapping of leaf rust resistance gene LrFun in Romanian wheat line Fundulea 900. Molecular Breeding, 33: 931–937.
 
Yuan J.H., Liu T.G., Chen W.Q. (2007): Postulation of leaf rust resistance genes in 47 new wheat cultivars at seedling stage. Scientia Agricultura Sinica, 40: 1925–1935.
 
Zhang H., Xia X.C., He Z.H., Li X., Li Z.F., Liu D.Q. (2011): Molecular mapping of leaf rust resistance gene LrBi16 in Chinese wheat cultivar Bimai 16. Molecular Breeding, 28: 527–534.
 
Zhao X. L., Zheng T. C., Xia X. C., He Z. H., Liu D. Q., Yang W. X., Yin G. H., Li Z. F. (2008): Molecular mapping of leaf rust resistance gene LrZH84 in Chinese wheat line Zhou 8425B. Theoretical and Applied Genetics, 117, 1069-1075 https://doi.org/10.1007/s00122-008-0845-9
 
Zhou Huixin, Xia Xianchun, He Zhonghu, Li Xing, Wang Cuifen, Li Zaifeng, Liu Daqun (2013): Molecular mapping of leaf rust resistance gene LrNJ97 in Chinese wheat line Neijiang 977671. Theoretical and Applied Genetics, 126, 2141-2147 https://doi.org/10.1007/s00122-013-2124-7
 
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