Karyotype analysis of Lablab purpureus (L.) Sweet using fluorochrome banding and fluorescence in situ hybridisation with rDNA probes

https://doi.org/10.17221/32/2015-CJGPBCitation:She C.-., Jiang X.-. (2015): Karyotype analysis of Lablab purpureus (L.) Sweet using fluorochrome banding and fluorescence in situ hybridisation with rDNA probes. Czech J. Genet. Plant Breed., 51: 110-116.
download PDF
The mitotic chromosomes of Lablab purpureus (L.) Sweet were characterised using sequential combined propidium iodide (PI) and 4',6-diamidino-2-phenylindole (DAPI) (CPD) staining and fluorescence in situ hybridisation (FISH) with 5S and 45S rDNA probes. The detailed karyotype of this species was established using prometaphase chromosomes. After CPD staining, CPD and DAPI+ bands were shown simultaneously. CPD bands occurred in the proximal regions of the long arms of all chromosome pairs and at all 45S rDNA sites, while the DAPI+ bands appeared in all centromeres. FISH with rDNA probes revealed one 5S locus and eight 45S loci. The single 5S locus was interstitially located on the long arms of the shortest chromosome pair. Among the 45S loci, two large loci were located in the secondary constrictions of the short arms of two chromosome pairs; six small or minimal loci were proximally located on the short or long arms of six chromosome pairs. Each prometaphase chromosome pair could be identified using the CPD and DAPI+ bands, the rDNA-FISH signals in combination with the chromosome measurements and condensation patterns. The karyotype was formulated as 2n = 2x = 22 = 14m (2SAT) + 6sm + 2st (2SAT), and the asymmetry indices, CI, A1, A2, As K%, AI and the Stebbins category were 38.23 ± 7.06, 0.36, 0.31, 61.99, 5.68 and 2B, respectively.
Ali MA, Hasan MM, Mia MS, Ahmad QN (2011): Karyotype analysis in lignosus bean (<i>Dipogon lignosus</i>) and lablab bean (<i>Lablab purpureus</i>). Journal of the Bangladesh Agricultural University, 9, -  https://doi.org/10.3329/jbau.v9i1.8740
Bonifácio Eliene Mariano, Fonsêca Artur, Almeida Cícero, dos Santos Karla G. B., Pedrosa-Harand Andrea (2012): Comparative cytogenetic mapping between the lima bean (Phaseolus lunatus L.) and the common bean (P. vulgaris L.). Theoretical and Applied Genetics, 124, 1513-1520  https://doi.org/10.1007/s00122-012-1806-x
Chaowen She, Liu Jingyu, Xiong Zhiyong, Song Yunchun (2004): Karyotype analysis of Psophocarpus tetragonolobus (L.) DC by chromosome banding and fluorescence in situ hybridization. Caryologia, 57, 387-394  https://doi.org/10.1080/00087114.2004.10589421
Chen R.Y. (2003): Chromosome Atlas of Major Economic Plants Genome in China (II). Beijing, Science Press. (in Chinese)
Cheng Z., Buell C.R., Wing R.A., Jiang J. (2002): Resolution of fluorescence in-situ hybridization mapping on rice mitotic prometaphase chromosomes, meiotic pachytene chromosomes and extended DNA fibers. Chromosome Research, 10: 379–387. https://doi.org/10.1023/A:1016849618707
Chinese Pharmacopoeia Commission (2010): Pharmacopoeia of the People’s Republic of China 2010 (Volume I). Beijing, Chinese Medicine Science and Technology Press. (in Chinese)
Chung Mei-Chu, Lee Yung-I, Cheng Yueh-Yun, Chou Yi-Jia, Lu Chia-Fu (2008): Chromosomal polymorphism of ribosomal genes in the genus Oryza. Theoretical and Applied Genetics, 116, 745-753  https://doi.org/10.1007/s00122-007-0705-z
Datson P.M., Murray B.G. (2006): Ribosomal DNA locus evolution in Nemesia: transposition rather than structural rearrangement as the key mechanism? Chromosome Research, 14: 845–857.
de A. Bortoleti Kyria Cilene, Benko-Iseppon Ana Maria, de Melo Natoniel Franklin, Brasileiro-Vidal Ana Christina (2012): Chromatin differentiation between Vigna radiata (L.) R. Wilczek and V. unguiculata (L.) Walp. (Fabaceae). Plant Systematics and Evolution, 298, 689-693  https://doi.org/10.1007/s00606-011-0551-y
de Moraes Ana Paula, dos Santos Soares Filho Walter, Guerra Marcelo (2007): Karyotype diversity and the origin of grapefruit. Chromosome Research, 15, 115-121  https://doi.org/10.1007/s10577-006-1101-2
Fuchs J&#x000F6;rg, K&#x000FC;hne Martina, Schubert Ingo (1998): Assignment of linkage groups to pea chromosomes after karyotyping and gene mapping by fluorescent in situ hybridization. Chromosoma, 107, 272-276  https://doi.org/10.1007/s004120050308
Fukui K., Iijima K. (1991): Somatic chromosome map of rice by imaging methods. Theoretical and Applied Genetics, 81, -  https://doi.org/10.1007/BF00226723
FUKUI Kiichi, MUKAI Yasuhiko (1988): Condensation pattern as a new image parameter for identification of small chromosomes in plants.. The Japanese journal of genetics, 63, 359-366  https://doi.org/10.1266/jjg.63.359
Fukui K., Nakayama S., Ohmido N., Yoshiaki H., Yamabe M. (1998): Quantitative karyotyping of three diploid Brassica species by imaging methods and localization of 45s rDNA loci on the identified chromosomes. Theoretical and Applied Genetics, 96, 325-330  https://doi.org/10.1007/s001220050744
Gerlach W.L., Dyer T.A. (1980): Sequence organization of the repeating units in the nucleus of wheat which contain 5S rRNA genes. Nucleic Acids Research, 8, 4851-4865  https://doi.org/10.1093/nar/8.21.4851
Guimaraes G., Cardoso L., Oliveira H., Santos C., Duarte P., Sottomayor M. (): Cytogenetic characterization and genome size of the medicinal plant Catharanthus roseus (L.) G. Don. AoB Plants, 2012, pls002-pls002  https://doi.org/10.1093/aobpla/pls002
Hasterok R., Jenkins G., Langdon T., Jones R. N., Maluszynska J. (2001): Ribosomal DNA is an effective marker of Brassica chromosomes. TAG Theoretical and Applied Genetics, 103, 486-490  https://doi.org/10.1007/s001220100653
Iwata Aiko, Greenland Christopher M., Jackson Scott A. (2013): Cytogenetics of Legumes in the Phaseoloid Clade. The Plant Genome, 6, 0-  https://doi.org/10.3835/plantgenome2013.03.0004
LEVAN ALBERT, FREDGA KARL, SANDBERG AVERY A. (1964): NOMENCLATURE FOR CENTROMERIC POSITION ON CHROMOSOMES. Hereditas, 52, 201-220  https://doi.org/10.1111/j.1601-5223.1964.tb01953.x
Li M.X., Chen R.Y. (1985): A suggestion on the standardization of karyotype analysis in plants. Journal of Wuhan Botanical Research, 3: 297–302. (in Chinese)
Li R.Q. (1989): Studies on Karyotypes of Vegetables in China. Wuhan, Wuhan University Press. (in Chinese)
Moscone Eduardo A, Klein Franz, Lambrou Maria, Fuchs Jörg, Schweizer Dieter (1999): Quantitative karyotyping and dual-color FISH mapping of 5S and 18S-25S rDNA probes in the cultivated Phaseolus species (Leguminosae). Genome, 42, 1224-1233  https://doi.org/10.1139/g99-070
Paszko B. (2006): A critical review and a new proposal of karyotype asymmetry indices. Plant Systematics and Evolution, 258, 39-48  https://doi.org/10.1007/s00606-005-0389-2
Perry Keith L., Palukaitis Peter (1990): Transcription of tomato ribosomal DNA and the organization of the intergenic spacer. MGG Molecular & General Genetics, 221, 102-112  https://doi.org/10.1007/BF00280374
Peterson D.G., Lapitan N.L.V., Stack S.M. (1999): Localization of single- and low-copy sequences on tomato synaptonemal complex spreads using fluorescence in situ hybridization (FISH). Genetics, 152: 427–439.
Raskina Olga, Belyayev Alexander, Nevo Eviatar (2004): Activity of the En/Spm-like transposons in meiosis as a base for chromosome repatterning in a small, isolated, peripheral population of Aegilops speltoides Tausch.. Chromosome Research, 12, 153-161  https://doi.org/10.1023/B:CHRO.0000013168.61359.43
Schubert I., Wobus U. (1985): In situ hybridization confirms jumping nucleolus organizing regions in Allium. Chromosoma, 92, 143-148  https://doi.org/10.1007/BF00328466
She C.W., Liu J.Y., Song Y.C. (2006): CPD staining: an effective technique for detection of NORs and other GC-rich chromosomal regions in plants. Biotechnic & Histochemistry, 81: 13–21.
She C.-W., Jiang X.-H., Ou L.-J., Liu J., Long K.-L., Zhang L.-H., Duan W.-T., Zhao W., Hu J.-C., Keurentjes J. (2015): Molecular cytogenetic characterisation and phylogenetic analysis of the seven cultivated Vigna species (Fabaceae). Plant Biology, 17, 268-280  https://doi.org/10.1111/plb.12174
Smartt J. (1990): Grain Legumes: Evolution and Genetic Resources. Cambridge, Cambridge University Press.
Zoldos V., Papes D., Cerbah M., Panaud O., Besendorfer V., Siljak-Yakovlev S. (1999): Molecular-cytogenetic studies of ribosomal genes and heterochromatin reveal conserved genome organization among 11 Quercus species. TAG Theoretical and Applied Genetics, 99, 969-977  https://doi.org/10.1007/s001220051404
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti