Comprehensive genomic analysis and expression profiling of the BTB and TAZ (BT) genes in cucumber (Cucumis sativus L.) Y., Li G., Zhang L., Xu J., Hu L., Jiang L., Liu S. (2020): Comprehensive genomic analysis and expression profiling of the BTB and TAZ (BT) genes in cucumber (Cucumis sativus L.). Czech J. Genet. Plant Breed., 56: 15-23.
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BTB-TAZ (BT) proteins are plant-specific transcription factors containing a BTB domain and a TAZ domain. They play vital roles in various biological processes and stress responses. In this study, a total of three BT genes (CsBT13) were identified from cucumber genome, and they were unevenly distributed in two of the seven chromosomes. Phylogenetic analysis of the BT proteins from cucumber, Arabidopsis, apple, tomato, and rice revealed that these proteins could be distinctly divided into two groups in accordance with their motif distributions. We also determined the structures of BT genes from cucumber, Arabidopsis, and rice to demonstrate their differences. The quantitative real-time PCR (qRT-PCR) results showed that the CsBT genes displayed differential expression patterns in cucumber tissues, and their expression was regulated by cold, salt, and drought stresses. These findings suggest that CsBT genes may participate in cucumber development and responses to various abiotic stresses.

An J.P., An X.H., Yao J.F., Wang X.N., You C.X., Wang X.F., Hao Y.J. (2018a): BTB protein MdBT2 inhibits anthocyanin and proanthocyanidin biosynthesis by triggering MdMYB9 degradation in apple. Tree Physiology, 38: 1578–1587.
An J.P., Li R., Qu F.J., You C.X., Wang X.F., Hao Y.J. (2018b): R2R3-MYB transcription factor MdMYB23 is involved in the cold tolerance and proanthocyanidin accumulation in apple. The Plant Journal, 96: 562–577.
An J.P., Yao J.F., Xu R.R., You C.X., Wang X.F., Hao Y.J. (2018c): Apple bZIP transcription factor MdbZIP44 regulates abscisic acid-promoted anthocyanin accumulation. Plant, Cell & Environment, 41: 2678–2692.
An J.P., Zhang X.W., Bi S.Q., You C.X., Wang X.F., Hao Y.J. (2019): MdbHLH93, an apple activator regulating leaf senescence, is regulated by ABA and MdBT2 in antagonistic ways. New Phytologist, 222: 735–751.
Araus V., Vidal E.A., Puelma T., Alamos S., Mieulet D., Guiderdoni E., Gutierrez R.A. (2016): Members of BTB gene family of scaffold proteins suppress nitrate uptake and nitrogen use efficiency. Plant Physiology, 171: 1523–1532.
Bai Y., Zhu W., Hu X., Sun C., Li Y., Wang D., Wang Q., Pei G., Zhang Y., Guo A., Zhao H., Lu H., Mu X., Hu J., Zhou X., Xie C.G. (2016): Genome-wide analysis of the bZIP gene family identifies two ABI5-like bZIP transcription factors, BrABI5a and BrABI5b, as positive modulators of ABA signalling in Chinese cabbage. PLOS ONE, 11: e0158966.
Cao J., Jiang M., Li P., Chu Z. (2016): Genome-wide identification and evolutionary analyses of the PP2C gene family with their expression profiling in response to multiple stresses in Brachypodium distachyon. BMC Genomics, 17: 175.
Chaharbakhshi E., Jemc J.C. (2016): Broad-complex, tramtrack, and bric-a-brac (BTB) proteins: Critical regulators of development. Genesis, 54: 505–518.
Chu Z., Wang X., Li Y., Yu H., Li J., Lu Y., Li H., Ouyang B. (2016): Genomic organization, phylogenetic and expression analysis of the B-BOX gene family in tomato. Frontiers in Plant Science, 7: 1552.
Du L., Poovaiah B.W. (2004): A novel family of Ca2+/calmodulin-binding proteins involved in transcriptional regulation: interaction with fsh/Ring3 class transcription activators. Plant Molecular Biology, 54: 549–569.
Figueroa P., Gusmaroli G., Serino G., Habashi J., Ma L., Shen Y., Feng S., Bostick M., Callis J., Hellmann H., Deng X.W. (2005): Arabidopsis has two redundant Cullin3 proteins that are essential for embryo development and that interact with RBX1 and BTB proteins to form multisubunit E3 ubiquitin ligase complexes in vivo. The Plant Cell, 17: 1180–1195.
Gingerich D.J., Gagne J.M., Salter D.W., Hellmann H., Estelle M., Ma L., Vierstra R.D. (2005): Cullins 3a and 3b assemble with members of the broad complex/tramtrack/bric-a-brac (BTB) protein family to form essential ubiquitin-protein ligases (E3s) in Arabidopsis. Journal of Biological Chemistry, 280: 18810–18821.
Gingerich D.J., Hanada K., Shiu S.H., Vierstra R.D. (2007): Large-scale, lineage-specific expansion of a bric-a-brac/tramtrack/broad complex ubiquitin-ligase gene family in rice. The Plant Cell, 19: 2329–2348.
Hao C.C., Jia J., Chen Z., Xing J.H., Weng Q.Y., Wang F.R., Dong J.G., Han J.M. (2013): Functional analysis of BT4 of Arabidopsis thaliana in resistance against Botrytis cinerea. Australasian Plant Pathology, 42: 393–401.
Li J., Su X., Wang Y., Yang W., Pan Y., Su C., Zhang X. (2018): Genome-wide identification and expression analysis of the BTB domain-containing protein gene family in tomato. Genes & Genomics, 40: 1–15.
Livak K.J., Schmittgen T.D. (2001): Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods, 25: 402–408.
Mandadi K.K., Misra A., Ren S., McKnight T.D. (2009): BT2, a BTB protein, mediates multiple responses to nutrients, stresses, and hormones in Arabidopsis. Plant Physiology, 150: 1930–1939.
Misra A., McKnight T.D., Mandadi K.K. (2018): Bromodomain proteins GTE9 and GTE11 are essential for specific BT2-mediated sugar and ABA responses in Arabidopsis thaliana. Plant Molecular Biology, 96: 393–402.
Ren S., Mandadi K.K., Boedeker A.L., Rathore K.S., McKnight T.D. (2007): Regulation of telomerase in Arabidopsis by BT2, an apparent target of TELOMERASE ACTIVATOR1. The Plant Cell, 19: 23–31.
Robert H.S., Quint A., Brand D., Vivian-Smith A., Offringa R. (2009): BTB and TAZ domain scaffold proteins perform a crucial function in Arabidopsis development. The Plant Journal, 58: 109–121.
Sato T., Maekawa S., Konishi M., Yoshioka N., Sasaki Y., Maeda H., Ishida T., Kato Y., Yamaguchi J., Yanagisawa S. (2017): Direct transcriptional activation of BT genes by NLP transcription factors is a key component of the nitrate response in Arabidopsis. Biochemical and Biophysical Research Communications, 483: 380–386.
Stogios P.J., Downs G.S., Jauhal J.J., Nandra S.K., Prive G.G. (2005): Sequence and structural analysis of BTB domain proteins. Genome Biology, 6: R82.
Wang X.F., An J.P., Liu X., Su L., You C.X., Hao Y.J. (2018a): The nitrate-responsive protein MdBT2 regulates anthocyanin biosynthesis by interacting with the MdMYB1 transcription factor. Plant Physiology, 178: 890–906.
Wang Y., Zhang Y., Zhou R., Dossa K., Yu J., Li D., Liu A., Mmadi M.A., Zhang X., You J. (2018b): Identification and characterization of the bZIP transcription factor family and its expression in response to abiotic stresses in sesame. PLOS ONE, 13: e0200850.
Xu G., Guo C., Shan H., Kong H. (2012): Divergence of duplicate genes in exon-intron structure. Proceedings of the National Academy of Sciences of the United States of America, 109: 1187–1192.
Zhao Q., Ren Y.R., Wang Q.J., Wang X.F., You C.X., Hao Y.J. (2016): Ubiquitination-related MdBT scaffold proteins target a bHLH transcription factor for iron homeostasis. Plant Physiology, 172: 1973–1988.
Zheng X., Xing J., Zhang K., Pang X., Zhao Y., Wang G., Zang J., Huang R., Dong J. (2019): Ethylene response factor ERF11 activates BT4 transcription to regulate immunity to Pseudomonas syringae. Plant Physiology, 180: 1132–1151.
Zhou Y., Hu L., Ye S., Jiang L., Liu S. (2018): Genome-wide identification and characterization of cysteine-rich polycomb-like protein (CPP) family genes in cucumber (Cucumis sativus) and their roles in stress responses. Biologia, 73: 425–435.
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