Characterization of a naturally occurring early-flowering rice mutant resulting from a novel variation in the Ghd7 locus

https://doi.org/10.17221/36/2021-CJGPBCitation:

Li W., Zheng C., Zhou J., Zhang Z., Zhou G., Xie X. (2021): Characterization of a naturally occurring early-flowering rice mutant resulting from a novel variation in the Ghd7 locus. Czech J. Genet. Plant Breed., 57: 166−169.

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Rice is one of the main staple food crops and is consumed by more than half of the global population. Optimizing the flowering time may help to maximize grain production. In this study, we characterized a rice mutant, Zixiangnuo early-flowering mutant (ZXN-E). Compared with the wild-type variety, Zixiangnuo (ZXN), the mutant plants flowered earlier, were shorter and produced smaller panicles. Bulked segregant analysis revealed a 7-bp deletion in the first exon of the Grain number, plant height, and heading date 7 (Ghd7) locus. The allele with the 7-bp deletion was not detected in the rice varieties included in the 3 000 Rice Genomes Project. Thus, the 7-bp deletion identified in the naturally occurring mutant ZXN-E is a novel variation. Our findings suggest that ZXN-E may be a useful germplasm for breeding new rice varieties.

References:
Cai M.H., Chen S.H., Wu M.M., Zheng T.H., Zhou L., Li C.N., Zhang H., Wang J.C., Xu X.Y., Chai J.T., Ren Y.L., Guo X.P., Zhang X., Lei C.L., Cheng Z.J., Wang J., Jiang L., Zhai H.Q., Wang H.Y., Zhu S.S., Wan J.M. (2019): Early heading 7 interacts with DTH8, and regulates flowering time in rice. Plant Cell Reports, 38: 521–532. https://doi.org/10.1007/s00299-019-02380-7
 
Hori K., Matsubara K., Yano M. (2016): Genetic control of flowering time in rice: integration of Mendelian genetics and genomics. Theoretical and Applied Genetics, 129: 2241–2252.  https://doi.org/10.1007/s00122-016-2773-4
 
Li X.M., Chao D.Y., Wu Y., Huang X.H., Chen K., Cui L.G., Su L., Ye W.W., Chen H., Chen H.C., Dong N.Q., Guo T., Shi M., Feng Q., Zhang P., Han B., Shan J.X., Gao J.P., Lin H.X. (2015): Natural alleles of a proteasome alpha2 subunit gene contribute to thermotolerance and adaptation of African rice. Nature Genetics, 47: 827–833. https://doi.org/10.1038/ng.3305
 
Lu L., Yan W.H., Xue W.Y., Shao D., Xing Y.Z. (2012): Evolution and association analysis of Ghd7 in rice. PLoS One, 7: e34021.
 
Wang W.S., Mauleon R., Hu Z.Q., Chebotarov D., Tai S., Wu Z., Li M., Zheng T.Q., Fuentes R.R., Zhang F., Mansueto L., Copetti D., Sanciangco M., Palis K.C., Xu J., Sun C., Fu B.Y., Zhang H., Gao Y., Zhao X., Shen F., Cui X., Yu H., Li Z., Chen M., Detras J., Zhou Y., Zhang X., Zhao Y., Kudrna D., Wang C., Li R., Jia B., Lu J., He X., Dong Z., Xu J., Li Y., Wang M., Shi J., Li J., Zhang D., Lee S., Hu W., Poliakov A., Dubchak I., Ulat V.J., Borja F.N., Mendoza J.R., Ali J., Li J., Gao Q., Niu Y., Yue Z., Naredo M.E.B., Talag J., Wang X., Li J., Fang X., Yin Y., Glaszmann J.C., Zhang J., Li J., Hamilton R.S., Wing R.A., Ruan J., Zhang G., Wei C., Alexandrov N., McNally K.L., Li Z.K., Leung H. (2018): Genomic variation in 3,010 diverse accessions of Asian cultivated rice. Nature, 557: 43–49. https://doi.org/10.1038/s41586-018-0063-9
 
Wu S., Qiu J., Gao Q. (2019): QTL-BSA: A bulked segregant analysis and visualization pipeline for QTL-seq. Interdisciplinary Sciences, Computational Life Sciences, 11: 730–737.
 
Xue W.Y., Xing Y.Z., Weng X.Y., Zhao Y., Tang W.J., Wang L., Zhou H.J., Yu S.B., Xu C.G., Li X.H., Zhang Q.F. (2008): Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice. Nature Genetics, 40: 761–767. https://doi.org/10.1038/ng.143
 
Zheng X.M., Feng L., Wang J., Qiao W.H, Zhang L., Cheng Y., Yang Q.W. (2016): Nonfunctional alleles of long-day suppressor genes independently regulate flowering time. Journal of Integrative Plant Biology, 58: 540–548. https://doi.org/10.1111/jipb.12383
 
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