Response of selected winter wheat cultivars to inoculation with different Mycosphaerella graminicola isolates

https://doi.org/10.17221/44/2015-CJGPBCitation:Šíp V., Chrpová J., Palicová J. (2015): Response of selected winter wheat cultivars to inoculation with different Mycosphaerella graminicola isolates. Czech J. Genet. Plant Breed., 51: 86-95.
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Five winter wheat cultivars, differing in resistance to Septoria tritici blotch (STB), were spray inoculated under field conditions for two years and at two locations with nine Mycosphaerella graminicola isolates and a mixture of isolates that were obtained from different regions of the Czech Republic. Main aims of this study were (i) to compare isolate, host cultivar and environmental effects on five traits indicative of STB severity and (ii) to analyse pathogen aggressiveness and host-pathogen relations for improving evaluation of cultivar resistance. ANOVA showed in all traits, except the reduction in 1000 grain weight, significant isolate effects. However, the effects of isolate and genotype by isolate interactions were much lower (2.3–4%) than cultivar (19.1–53.7%) and environmental (11.9–58.6%) effects. Cultivar resistance to the disease limited much the loss in grain weight per spike to a halve, from 34.7% in the susceptible Bakfis to 17.3% in the resistant Arina. Visual scoring of symptoms in the middle and at the end of disease development (performed on the 1–9 scale), reflecting the disease progress and infected leaf area, showed the highest cultivar effect (54%) and could be recommended for evaluation of cultivar resistance in breeding practice. All examined traits were significantly interrelated, but significant differences between all the five cultivars were only detected after examination of the % coverage of flag leaves with lesions bearing pycnidia. Resistance in the cultivar Arina was detected by all isolates and the isolate mixture. In spite of significant differences in classification of resistances in the cultivars Bohemia and Mulan after inoculation with one isolate (1081), specific interactions between cultivars and isolates collected in this Central European region are rare. The study leads to a conclusion that investigation into stability of STB resistance across a wide range of environments is more valuable for breeding purposes than the study of cultivar response to different isolates. Combination of important isolate properties in a mixture of isolates is stressed as well.
References:
Abrinbana Masoud, Mozafari Javad, Shams-bakhsh Masoud, Mehrabi Rahim (2012): Resistance spectra of wheat genotypes and virulence patterns of Mycosphaerella graminicola isolates in Iran. Euphytica, 186, 75-90  https://doi.org/10.1007/s10681-011-0493-z
 
Arraiano L. S., Brown J. K. M. (2006): Identification of isolate-specific and partial resistance to septoria tritici blotch in 238 European wheat cultivars and breeding lines. Plant Pathology, 55, 726-738  https://doi.org/10.1111/j.1365-3059.2006.01444.x
 
Arraiano L. S., Chartrain L., Bossolini E., Slatter H. N., Keller B., Brown J. K. M. (2007): A gene in European wheat cultivars for resistance to an African isolate of Mycosphaerella graminicola. Plant Pathology, 56, -  https://doi.org/10.1111/j.1365-3059.2006.01499.x
 
Brading Penny A., Verstappen Els C. P., Kema Gert H. J., Brown James K. M. (2002): A Gene-for-Gene Relationship Between Wheat and Mycosphaerella graminicola , the Septoria Tritici Blotch Pathogen. Phytopathology, 92, 439-445  https://doi.org/10.1094/PHYTO.2002.92.4.439
 
Brown J. K. M., Kema G. H. J., Forrer H.-R., Verstappen E. C. P., Arraiano L. S., Brading P. A., Foster E. M., Fried P. M., Jenny E. (2001): Resistance of wheat cultivars and breeding lines to septoria tritici blotch caused by isolates of Mycosphaerella graminicola in field trials. Plant Pathology, 50, 325-338  https://doi.org/10.1046/j.1365-3059.2001.00565.x
 
Chartrain L., Brading P.A., Widdowson J.P., Brown J.K.M. (2004a): Partial resistance to Septoria tritici blotch (Mycosphaerella graminicola) in wheat cultivars Arina and Riband. Phytopathology, 94: 497–504.
 
Chartrain L., Brading P.A., Makepeace J.C., Brown J.K.M. (2004b): Sources of resistance to Septoria tritici blotch and implications for wheat breeding. Plant Pathology, 53: 454–460.
 
Chartrain L., Sourdille P., Bernard M., Brown J. K. M. (2009): Identification and location of Stb9 , a gene for resistance to septoria tritici blotch in wheat cultivars Courtot and Tonic. Plant Pathology, 58, 547-555  https://doi.org/10.1111/j.1365-3059.2008.02013.x
 
Cools Hans J, Fraaije Bart A (2008): Are azole fungicides losing ground against Septoria wheat disease? Resistance mechanisms inMycosphaerella graminicola. Pest Management Science, 64, 681-684  https://doi.org/10.1002/ps.1568
 
Drabešová Jana, Ryšánek Pavel, Brunner Patrick, McDonald Bruce A., Croll Daniel (2013): Population genetic structure of Mycosphaerella graminicola and Quinone Outside Inhibitor (QoI) resistance in the Czech Republic. European Journal of Plant Pathology, 135, 211-224  https://doi.org/10.1007/s10658-012-0080-8
 
Eberhart S. A., Russell W. A. (1966): Stability Parameters for Comparing Varieties1. Crop Science, 6, 36-  https://doi.org/10.2135/cropsci1966.0011183X000600010011x
 
Eyal Z., Scharen A.L., Prescott J.M., van Ginkel M. (1987): The Septoria Diseases of Wheat: Concepts and Methods of Disease Management. Mexico, CIMMYT.
 
Tabib Ghaffary S. Mahmod, Faris Justin D., Friesen Timothy L., Visser Richard G. F., van der Lee Theo A. J., Robert Olivier, Kema Gert H. J. (2012): New broad-spectrum resistance to septoria tritici blotch derived from synthetic hexaploid wheat. Theoretical and Applied Genetics, 124, 125-142  https://doi.org/10.1007/s00122-011-1692-7
 
Goudemand Ellen, Laurent Valérie, Duchalais Laure, Tabib Ghaffary Seyed Mahmod, Kema Gert H. J., Lonnet Philippe, Margalé Eric, Robert Olivier (2013): Association mapping and meta-analysis: two complementary approaches for the detection of reliable Septoria tritici blotch quantitative resistance in bread wheat (Triticum aestivum L.). Molecular Breeding, 32, 563-584  https://doi.org/10.1007/s11032-013-9890-4
 
Keller B., Feuillet C., Messmer M. (2000): Genetics of disease resistance. In: Slusarenko A.J., Fraser R.S.S., Loon L.C. (eds): Mechanisms of Resistance to Plant Diseases. Dordrecht, Kluwer Academic Publishers: 101–160.
 
Kemma G.H.J., Annone J.G., Sayoud R., van Silfhout C.H., van Ginkel M., de Bree J. (1996): Genetic variation for virulence and resistance in the wheat-Mycosphaerella graminicola pathosystem I. Interactions between pathogen isolates and host cultivars. Pathology, 86: 200–212.
 
ORTON ELIZABETH S., DELLER SIAN, BROWN JAMES K. M. (2011): Mycosphaerella graminicola: from genomics to disease control. Molecular Plant Pathology, 12, 413-424  https://doi.org/10.1111/j.1364-3703.2010.00688.x
 
Risser P., Ebmeyer E., Korzun V., Hartl L., Miedaner T. (2011): Quantitative Trait Loci for Adult-Plant Resistance to Mycosphaerella graminicola in Two Winter Wheat Populations. Phytopathology, 101, 1209-1216  https://doi.org/10.1094/PHYTO-08-10-0203
 
Schilly Ayla, Risser Peter, Ebmeyer Erhard, Hartl Lorenz, Reif Jochen C., Würschum Tobias, Miedaner Thomas (2011): Stability of Adult-plant Resistance to Septoria tritici blotch in 24 European Winter Wheat Varieties Across Nine Field Environments. Journal of Phytopathology, , no-no  https://doi.org/10.1111/j.1439-0434.2010.01783.x
 
Šíp V., Stuchlíková E., Chrpová J. (2001): The response of selected winter wheat cultivars to artificial infection with Septoria tritici under field conditions. Czech Journal of Genetics and Plant Breeding, 37: 73–81.
 
Šíp V., Chrpová J., Štočková L. (2011): Evaluation of resistance to Fusarium head blight in wheat using different sources of inoculum. Czech Journal of Genetics and Plant Breeding, 47: 131–139.
 
Šíp V., Chrpová J., Palicová J., Hanzalová A., Čejka L. (2014): Methodology of Testing Wheat Resistance to Brown Leaf Spot Diseases under Field Conditions. Prague-Ruzyně, Crop Research Institute. (in Czech)
 
Suffert F., Sache I., Lannou C. (2013): Assessment of quantitative traits of aggressiveness in Mycosphaerella graminicola on adult wheat plants. Plant Pathology, 62, 1330-1341  https://doi.org/10.1111/ppa.12050
 
Torriani Stefano FF, Brunner Patrick C, McDonald Bruce A, Sierotzki Helge (2009): QoI resistance emerged independently at least 4 times in European populations of Mycosphaerella graminicola. Pest Management Science, 65, 155-162  https://doi.org/10.1002/ps.1662
 
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