Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection T., Chankaew S., Monkham T., Saksirirat W., Sanitchon J. (2017): Pyramiding of four blast resistance QTLs into Thai rice cultivar RD6 through marker-assisted selection. Czech J. Genet. Plant Breed., 53: 1-8.
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Thai rice cultivar RD6 is well known for its cooking and eating qualities. However it is susceptible to blast disease, a major rice disease caused by the fungus Magnaporthe oryzae. This study focused on the pyramiding of four QTLs for blast resistance located on chromosomes 1, 2, 11 and 12, from two RD6 introgression lines. Marker-assisted selection was performed and facilitated the selection with 8 microsatellite flanking markers to enable the selection in BC2F2:3 lines. All possible combinations of the four QTL alleles were assessed for blast resistance by artificial inoculation using 8 diverse isolates in a greenhouse and under field conditions using the upland short row method. The results showed that the RD6 introgression lines carrying a high number of QTLs for blast resitance achieved from pyramiding have high levels of blast resistance and broad spectrum of resistance to the blast pathogens prevalent in the region. Only one of the M. oryzae isolates, THL185, was virulent to all the breeding lines, suggesting that the identification of new blast resistance genes or QTLs and pyramiding them into RD6 for durable blast resistance and no yield penalty should be the focus of further research.
Ahn S.W. (1994): International collaboration on breeding for resistance to rice blast. In: Zeigler R.S., Leong S.A., Teng P.S. (eds): Rice Blast Disease. Wallingford, Madison, CABI, CABI UK: 137–153.
Arunakanthi B., Srinivasprasad M., Madhanmohan K., Balachandran S.M., Madhav M.S., Reddy C.S., Viraktamath B.C. (2008): Introgression of major blast resistance genes Pi-1, Pi-2 and Pi-kh in Indica rice cultivars Samba Mahsuri and Swarna. The Journal of Mycology and Plant Pathology, 38: 625–630.
Ashkani Sadegh, Rafii Mohd Yusop, Rusli Ibrahim, Sariah Meon, Abdullah Siti Nor Akmar, Abdul Rahim Harun, Latif M. A. (2012): SSRs for Marker-Assisted Selection for Blast Resistance in Rice (Oryza sativa L.). Plant Molecular Biology Reporter, 30, 79-86
Flor H H (1971): Current Status of the Gene-For-Gene Concept. Annual Review of Phytopathology, 9, 275-296
Hittalmani S., Parco A., Mew T. V., Zeigler R. S., Huang N. (2000): Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice. TAG Theoretical and Applied Genetics, 100, 1121-1128
IRRI (1996): Standard Evaluation System for Rice. 4th Ed. Manila, IRRI.
Ishizaki Kazuhiko, Hoshi Toyokazu, Abe Sei-ichi, Sasaki Yukio, Kobayashi Kazuyuki, Kasaneyama Hironobu, Matsui Taka-aki, Azuma Satoshi (2005): Breeding of Blast Resistant Isogenic Lines in Rice Variety “Koshihikari” and Evaluation of Their Characters. Breeding Science, 55, 371-377
Khambanonda P. (1978): Mutation breeding in rice for high yield and better blast resistance. Thai Journal of Agricultural Science, 11: 263–271.
Khush G.S., Jena K.K. (2009): Current status and future prospects for research on blast resistance in rice (Oryza sativa L.). In: Wang G.L., Valent B. (eds): Advances in Genetics. Genomics and Control of Rice Blast Disease. New York, Springer: 1–10.
KOIDE Yohei, KOBAYASHI Nobuya, XU Donghe, FUKUTA Yoshimichi (2009): Resistance Genes and Selection DNA Markers for Blast Disease in Rice (Oryza sativa L.). Japan Agricultural Research Quarterly: JARQ, 43, 255-280
Koizumi S. (2007): Durability of resistance to rice blast disease. Japan International Research Center for Agricultural Sciences Working Report, 53: 1–10.
Korinsak S.(2009): Identification of blast resistance QTLs in two rice RIL populations and marker-assisted selection for pyramiding of four QTLs in RD6 rice variety. [M.S. Thesis.] Bangkok, Kasetsart University.
LIU JINLING, WANG XUEJUN, MITCHELL THOMAS, HU YAJUN, LIU XIONGLUN, DAI LIANGYING, WANG GUO-LIANG (2010): Recent progress and understanding of the molecular mechanisms of the rice- Magnaporthe oryzae interaction. Molecular Plant Pathology, 11, 419-427
Miah G., Rafii M. Y., Ismail M. R., Puteh A. B., Rahim H. A., Asfaliza R., Latif M. A. (2013): Blast resistance in rice: a review of conventional breeding to molecular approaches. Molecular Biology Reports, 40, 2369-2388
Noenplab A., Vanavichit A., Toojinda T., Sirithunya P., Tragoonrung S., Sriprakhon S., Vongsaprom C. (2006): QTL mapping for leaf and neck blast resistance in Khao Dawk Mali105 and Jao Hom Nin recombinant inbred lines. Science Asia, 32: 133–142.
Ou S.H. (1985): Blast. In: Rice Disease. 2nd Ed., Kew, Commonwealth Mycological Institute: 109–201.
Roumen E., Levy M., Nottegham J.L. (1997): Characterization of the European pathogen population of Magnaporthe grisea by DNA fingerprinting and pathotype analysis. European Journal of Plant Pathology, 103: 363–371.
Ruengphayak Siriphat, Chaichumpoo Ekawat, Phromphan Supaporn, Kamolsukyunyong Wintai, Sukhaket Wissarut, Phuvanartnarubal Ekapol, Korinsak Siripar, Korinsak Siriporn, Vanavichit Apichart (2015): Pseudo-backcrossing design for rapidly pyramiding multiple traits into a preferential rice variety. Rice, 8, -
Saka Norikuni (2006): A Rice (Oryza sativa L.) Breeding for Field Resistance to Blast Disease (Pyricularia oryzae) in Mountainous Region Agricultural Research Institute, Aichi Agricultural Research Center of Japan. Plant Production Science, 9, 3-9
Servin B. (2004): Toward a Theory of Marker-Assisted Gene Pyramiding. Genetics, 168, 513-523
Silprakhon S. (2004): Identification and mapping genes controlling leaf blast resistance in double haploid lines IR64 × Azucena population. In: Vanavichit A. (ed.): Proc.1st Conf. Rice for the Future, Bangkok.
Sirithanya P. (1998): Mapping gene controlling blast resistance in rice (Oryza sativa L.). [Ph.D. Thesis.] Bangkok, Kasetsart University.
Sirithunya P., Sreewongchai T., Sriprakhon S., Toojinda T., Pimpisithavorn S., Kosawang C., Smitamana P. (2008): Assessment of Genetic Diversity in Thai Isolates of Pyricularia grisea by Random Amplification of Polymorphic DNA. Journal of Phytopathology, 156, 196-204
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