Freezing tolerance and proline content of in vitro selected hydroxyproline resistant winter oilseed rape
A. Janská, S. Zelenková, M. Klíma, M. Vyvadilová, T.I. Prášilhttps://doi.org/10.17221/52/2009-CJGPBCitation:Janská A., Zelenková S., Klíma M., Vyvadilová M., Prášil T.I. (2010): Freezing tolerance and proline content of in vitro selected hydroxyproline resistant winter oilseed rape. Czech J. Genet. Plant Breed., 46: 35-40.
Twelve doubled haploid (DH) winter oilseed rape plants with altered levels of proline and/or freezing tolerance were obtained by in vitro selection for resistance to trans-4-hydroxy-l-proline (Hyp) in five segregating microspore populations. No significant response to selection either in proline content or in freezing tolerance, compared with the non-selected control populations, was observed. When data from all examined materials were combined, a weak correlation between proline content and freezing tolerance was observed.Keywords:
abiotic stress; Brassica napus; microspore culture; proline