Detection of genetic relationships among spring and winter triticale (× Triticosecale Witt.) and rye cultivars (Secale cereale L.) by using retrotransposon-based markers
A. Trebichalský, R. Kalendar, A. Schulman, O. Stratula, Z. Gálová, Ž. Balážová, M. Chňapekhttps://doi.org/10.17221/56/2013-CJGPBCitation:Trebichalský A., Kalendar R., Schulman A., Stratula O., Gálová Z., Balážová Ž., Chňapek M. (2013): Detection of genetic relationships among spring and winter triticale (× Triticosecale Witt.) and rye cultivars (Secale cereale L.) by using retrotransposon-based markers. Czech J. Genet. Plant Breed., 49: 171-174.
In the present research, we aimed to detect and evaluate the level of long terminal repeat (LTR) retrotransposons (WIS2, Wilma, Daniela, and Wham) intraspecific variability and intron polymorphism for β-amylase (BAMY) genes in 37 winter and 25 spring triticale cultivars coming from European countries and the USA and 5 Finnish rye cultivars. The triticale and rye genotypes differ significantly with respect to the patterns of the four explored LTR retrotransposons. A neighbour-joining dendrogram has separated all triticale and rye cultivars into three principal clusters: all winter triticale, all spring triticale and all rye cultivars. We have proved that retrotransposon-based markers can be used for differentiation of triticale and rye cultivars.Keywords:
β-amylase (BAMY) genes; long terminal repeat (LTR) genes; genetic diversity; molecular markers; rye; triticale