Molecular characterization of snowdrop lectin (GNA) and its comparison with reported lectin sequences of Amaryllidaceae S.D., Bakhsh A., Sancak C., Özcan S. (2016): Molecular characterization of snowdrop lectin (GNA) and its comparison with reported lectin sequences of Amaryllidaceae. Czech J. Genet. Plant Breed., 52: 94-100.
download PDF

Plant lectins have become efficient sources of insect resistance in crops. The present study was conducted to identify, amplify, clone and characterize the plant lectin gene GNA. The lectin, present in Galanthus nivalis (snowdrop), is an agglutinin toxic to hemiptera. The attempt was made to elucidate the relationship of the lectin gene trGNA (GNA isolated and characterized from Turkey) with other previously cloned lectins having insecticidal activity and to ensure the presence of the conserved mannose-binding region/site in the gene sequence. The full-length cDNA of trGNA was 477 bp that contained a 333 bp open reading frame encoding 157 amino acid proteins with 23 amino acids of signal peptide. BLAST results showed that trGNA has 89–97% similarity with previously reported GNA sequences while it has 84–96% similarity with earlier reported GNA protein sequences. No intron was detected within the region of genomic sequence corresponding to trGNA full-length cDNA. According to the search results from the NCBI (National Centrer for Biotechnology Information database), trGNA from Galanthus nivalis is most similar to the previously reported lectin sequences of Narcissus tazetta with a similarity percentage of 87%. The obtained results are useful for engineering of plants with enhanced insecticidal activity against chewing and sucking insects, causing crop pests. In addition, medical application of lectins may also be considered.

BAKHSH Allah, KHABBAZI Saber Delpasand, BALOCH Faheem Shahzad, DEMİREL Ufuk, ÇALIŞKAN Mehmet Emin, HATİPOĞLU Rüştü, ÖZCAN Sebahattin, ÖZKAN Hakan (2015): Insect-resistant transgenic crops: retrospect and challenges. TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, 39, 531-548
Czapla T. H., Lang B. A. (1990): Effect of Plant Lectins on the Larval Development of European Corn Borer (Lepidoptera: Pyralidae) and Southern Corn Rootworm (Coleoptera: Chrysomelidae). Journal of Economic Entomology, 83, 2480-2485
Gao Zhi M., Zheng Bo, Wang Wen Y., Li Qiang, Yuan Qi P. (2011): Cloning and functional characterization of a GNA-like lectin from Chinese Narcissus (Narcissus tazetta var. Chinensis Roem). Physiologia Plantarum, 142, 193-204
Gatehouse A.M.R., Hilder V.A., Boulter D. (1992): Potential of plant derived genes in the genetic manipulation of the crops for insect resistance. In: Gatehouse A.M.R., Hilder V.A., Boulter D. (eds): Plant Genetic Manipulation for Crop Protection. Wallingford, CAB International: 155–181.
Hayashi A., Saito T., Mukai Y., Kurita S., Hori T. (2005): Genetic variation in Lycoris radiata var. radiata in Japan. Genes & Genetic Systems, 80: 199–212.
Peumans W.J., Van Damme E.J.M. (1996): Prevalence, biological activity and genetic manipulation of lectins in foods. Trends in Food Science & Technology, 7: 132–138.
Pusztai Arpad, Ewen Stanley W. B., Grant George, Peumans Willy J., Damme Els J. M., Coates Marie E., Bardocz Susan (1995): Lectins and also bacteria modify the glycosylation of gut surface receptors in the rat. Glycoconjugate Journal, 12, 22-35
Pusztai Arpad, Koninkx Jos, Hendriks Henno, Kok Wouter, Hulscher Saskia, Van Damme Els J.M., Peumans Willy J., Grant George, Bardocz Susan (1996): Effect of the insecticidal Galanthus nivalis agglutinin on metabolism and the activities of brush border enzymes in the rat small intestine. The Journal of Nutritional Biochemistry, 7, 677-682
Smeets K., Van Damme E.J.M., Verhaert P., Barre A., Rouge P., Leuven F.V., Peumans W.J. (1997): Isolation, characterization and molecular cloning of the mannose-binding lectins from leaves and roots of garlic (Allium sativum L.). Plant Molecular Biology, 33: 223–234.
Van Damme Els J.M., Allen Anthony K., Peumans Willy J. (1987): Isolation and characterization of a lectin with exclusive specificity towards mannose from snowdrop (Galanthus nivalis) bulbs. FEBS Letters, 215, 140-144
DAMME Els J. M., KAKU Hanae, PERINI Fulvio, GOLDSTEIN Irwin J., PEETERS Ben, YAGI Fumio, DECOCK Benny, PEUMANS Willy J. (1991): Biosynthesis, primary structure and molecular cloning of snowdrop (Galanthus nivalis L.) lectin. European Journal of Biochemistry, 202, 23-30
DAMME Els J. M., SMEETS Koen, TORREKENS Sophie, LEUVEN Fred, PEUMANS Willy J. (1992): Isolation and characterization of alliinase cDNA clones from garlic (Allium sativum L.) and related species. European Journal of Biochemistry, 209, 751-757
Van Damme E.J.M., Smeets K., Engelborghs I., Aelbers H., Balzarini J., Pusztai A., Van Leuven F., Goldstein I.J., Peumans W.J. (1993a): Cloning and characterization of the lectin cDNA clones from onion, shallot and leek. Plant Molecular Biology, 23: 365–376.
Van Damme E.J.M., Smeets K., Torrekens S., Van Leuven F., Peumans W.J. (1993b): The mannose-specific lectins from ramsons (Allium ursinum L.) are encoded by three sets of genes. European Journal of Biochemistry, 217: 123–129.
Van Damme Els J. M., Smeets Koen, Van Leuven Fred, Peumans Willy J. (1994): Molecular cloning of mannose-binding lectins from Clivia miniata. Plant Molecular Biology, 24, 825-830
Van Damme E. (): The major tuber storage protein of araceae species is a lectin. Characterization and molecular cloning of the lectin from Arum maculatum L. PLANT PHYSIOLOGY, 107, 1147-1158
Vandenborre Gianni, Smagghe Guy, Van Damme Els J.M. (2011): Plant lectins as defense proteins against phytophagous insects. Phytochemistry, 72, 1538-1550
download PDF

© 2022 Czech Academy of Agricultural Sciences | Prohlášení o přístupnosti