Marzougui S. (2021): Allelic variations at the HvSNF2 and HvBM5 loci are associated with the heading date and growth habit of barley (Hordeum vulgare L.) under a semi-arid climate. Czech J. Genet. Plant Breed., 57: 76−79.
The heading date and growth habit are key factors that regulate the transition from the vegetative to the reproductive stage in barley. In this study, we used PCR based markers to identify the allelic variations in the Vrn-H1 (HvMB5) and Vrn-H2 (HvSNF2) genes and to predict the heading date and growth habit of a collection of Tunisian barley assessed under a semi-arid climate. The allelic variation at HvBM5 revealed two PCR fragments at 830 and 344 bp. Primer sets used to amplify the HvSNF2 gene have resulted in different alleles size of 543, 623, and 700 bp. Different allelic combinations of HVBM5 and HvSNF2 were associated with the heading date and growth habit. The spring and early heading accessions were only characterised by the amplification of the HvSNF2 fragment at 700 bp. All the winter accessions yielded the PCR product HvBM5 at 830 bp, but the variation in the heading date was determined by the HvSNF2 alleles. These DNA markers will be a powerful tool to predict the heading date and growth habit and can be used as markers for the assisted selection to speed up the national breeding programme.
allelic combinations; days to heading; growth habit; long-day conditions; vernalisation; Vrn-H loci
Cockram J., Norris C., O’Sullivan D.M. (2009): PCR-based markers diagnostic for spring and winter seasonal growth habit in barley. Crop Science, 49: 403–410. https://doi.org/10.2135/cropsci2008.07.0398
Dubcovsky H., Chen C., Yan L. (2005): Molecular characterization of the allelic variation at the Vrn-H2 vernalization locus in barley. Molecular Breeding, 15: 395–407. https://doi.org/10.1007/s11032-005-0084-6
Karsai I., Szcs P., Mészáros K., Filichkina T., Hayes P.M., Skinner J.S., Láng L., Bed Z. (2005): The Vrn-H2 locus is a major determinant of flowering time in a facultative × winter growth habit barley (Hordeum vulgare L.) mapping population. Theoretical and Applied Genetics, 110: 1458–1462. https://doi.org/10.1007/s00122-005-1979-7
Mohammadi M., Torkamaneh D., Nikkhah H.R. (2013): Correlation of vernalization loci VRN-H1 and VRN-H2 and growth habit in barley germplasm. International Journal of Plant Genomics, 2013: 924043. https://doi.org/10.1155/2013/924043
Szucs P., Skinner J.S., Karsai I., Cuesta-Marcos A., Haggard K.G., Corey A.E., Chen T.H., Hayes P.M. (2007): Validation of the Vrn-H2/Vrn-H1 epistatic model in barley reveals that intron length variation in Vrn-H1 may account for a continuum of vernalization sensitivity. Molecular Genetics and Genomics, 277: 249–261. https://doi.org/10.1007/s00438-006-0195-8
Taheripourfard Z.S., Izadi-Darbandi A., Ghazvini H., Ebrahimi M., Mortazavian S.M.M. (2018): Characterization of specific DNA markers at VRN-H1 and VRN-H2 loci for growth habit of barley genotypes. Journal of Genetics, 97: 87–95. https://doi.org/10.1007/s12041-018-0886-z
Takahashi R., Yasuda S. (1971): Genetics of earliness and growth habit in barley. In: Nilan R.A. (ed.): Proc. 2nd Int. Barley Genetic Symposium, Pullman: 388–408.
Trevaskis B., Hemming M.N., Peacock W.J., Dennis E.S. (2006): HvVrn2 responds to day length, whereas HvVrn1 is regulated by vernalization and developmental status. Plant Physiology, 140: 1397–1405. https://doi.org/10.1104/pp.105.073486
von Zitzewitz J., Szucs P., Dubcovsky J., Yan L., Francia E., Pecchioni N., Casas A., Chen T.H., Hayes P.M., Skinner J.S. (2005): Molecular and structural characterization of barley vernalization genes. Plant Molecular Biology, 59: 449–467. https://doi.org/10.1007/s11103-005-0351-2