Molecular identification of wheat leaf rust resistance genes in sixty Chinese wheat cultivars

https://doi.org/10.17221/6/2016-CJGPBCitation:Ren Z., Li Z., Shi L., Wang X., Zhu L., Li X., Liu D. (2018): Molecular identification of wheat leaf rust resistance genes in sixty Chinese wheat cultivars. Czech J. Genet. Plant Breed., 54: 1-8.
download PDF

Common wheat (Triticum aestivum L.) is the major crop cultivated in Xinjiang and Anhui provinces of China. The climate in these two provinces is favourable for wheat leaf rust (Puccinia triticina) (Pt) infection. Here, we demonstrate a detailed investigation on the leaf rust resistance of 60 major wheat cultivars cultivated in these two regions. A mixture of high virulent Pt races (THTT, THTS, THTQ and PHPS) were used to phenotype all the collected wheat cultivars at an adult plant stage. Phenotypic disease severity (FDS) and the area under the disease progress curve (AUDPC) for each of these wheat cultivars were calculated. Among all the tested wheat cultivars, three cultivars (Xindong20, Xindong 29 and 99AR142-1) with the lowest FDS and AUDPC may carry major resistance genes. Twenty-seven cultivars (45% of the total tested ones) showed a relatively lower resistance with an average of 12.52% FDS and 126.3 AUDPC. Minor resistance or slow rusting genes may be present in this group of cultivars. Molecular markers for leaf rust resistance genes Lr1, Lr9, Lr19, Lr24, Lr26 and Lr34 were further used for the genotypic screening. Lr1, Lr19, Lr26 and Lr34 were detected in 19 (31.7%), 1 (1.7%), 12 (20%) and 6 (10%) wheat cultivars, respectively. Neither Lr9 nor Lr24 could be detected in any of the tested cultivars. These results will greatly improve wheat molecular breeding for leaf rust resistance in these areas.

References:
Bartos P., Stuchlikova E., Kubova R. (1984): Wheat leaf rust epidemics in Czechoslovakia in 1983. Cereal Rusts Bulletin, 12: 40–41.
 
Broers L.H.M., Cuesta S.X., Lopez A.R.M. (1996): Field assessment of quantitative resistance to yellow rust in ten spring bread wheat cultivars. Euphytica, 90: 9–16.
 
Chai J. F., Zhou R. H., Jia J. Z., Liu X. (2006): Development and application of a new codominant PCR marker for detecting 1BL.1RS wheat-rye chromosome translocations. Plant Breeding, 125, 302-304 https://doi.org/10.1111/j.1439-0523.2006.01186.x
 
Chen W.Q., Qin Q.M., Chen Y.L., Yan S.B. (1998): Virulence dynamics of Puccinia recondita f.sp. tritici in China during 1992–1996. Acta Phytopathologica Sinica, 28: 101–106.
 
Cloutier Sylvie, McCallum Brent D., Loutre Caroline, Banks Travis W., Wicker Thomas, Feuillet Catherine, Keller Beat, Jordan Mark C. (2007): Leaf rust resistance gene Lr1, isolated from bread wheat (Triticum aestivum L.) is a member of the large psr567 gene family. Plant Molecular Biology, 65, 93-106 https://doi.org/10.1007/s11103-007-9201-8
 
Dong J.G. (2001): Agricultural Plant Pathology. China Agriculture Press, Beijing.
 
Dyck P. L. (1987): The association of a gene for leaf rust resistance with the chromosome 7D suppressor of stem rust resistance in common wheat. Genome, 29, 467-469 https://doi.org/10.1139/g87-081
 
Gupta Sudhir Kumar, Charpe Ashwini, Koul Sunita, Prabhu Kumble Vinod, Haq Qazi Mohd. Rizwanul (2005): Development and validation of molecular markers linked to an Aegilops umbellulata –derived leaf-rust-resistance gene, Lr9 , for marker-assisted selection in bread wheat. Genome, 48, 823-830 https://doi.org/10.1139/g05-051
 
Gupta S.K., Charpe A., Prabhu K.V., Haque Q.M. (2006a): Identification and validation of molecular markers linked to the leaf rust resistance gene Lr19 in wheat. Theoretical and Applied Genetics, 113: 1027–1036.
 
Gupta S.K., Charpe A., Koul S., Haque Q., Prabhu K. (2006b): Development and validation of SCAR markers cosegregating with an Agropyron elongatum derived leaf rust resistance gene Lr24 in wheat. Euphytica, 150: 233–240.
 
de Froidmont D. (1998): A Co-dominant Marker for the 1BL/1RS Wheat-rye Translocation via Multiplex PCR. Journal of Cereal Science, 27, 229-232 https://doi.org/10.1006/jcrs.1998.0194
 
Huerta-Espino J., Singh R. P., Germán S., McCallum B. D., Park R. F., Chen W. Q., Bhardwaj S. C., Goyeau H. (2011): Global status of wheat leaf rust caused by Puccinia triticina. Euphytica, 179, 143-160 https://doi.org/10.1007/s10681-011-0361-x
 
Knott D.R. (1989): The transfer of rust resistance from alien species to wheat. In: The Wheat Rust — Breeding for Resistance. Monographs on Theoretical and Applied Genetics, Berlin, Springer-Verlag: 162–181.
 
Lagudah E. S., McFadden H., Singh R. P., Huerta-Espino J., Bariana H. S., Spielmeyer W. (2006): Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theoretical and Applied Genetics, 114, 21-30 https://doi.org/10.1007/s00122-006-0406-z
 
Li Z.Q., Shang H.S. (2005): China’s Crop Disease Resistance and its Use. Beijing, China Agriculture Press: 208–209.
 
Li Z. F., Xia X. C., He Z. H., Li X., Zhang L. J., Wang H. Y., Meng Q. F., Yang W. X., Li G. Q., Liu D. Q. (2010): Seedling and Slow Rusting Resistance to Leaf Rust in Chinese Wheat Cultivars. Plant Disease, 94, 45-53 https://doi.org/10.1094/PDIS-94-1-0045
 
Line R.F., Chen X.M. (1995): Success in breeding for and managing durable resistance to wheat rusts. Plant Disease, 79: 1254–1255.
 
Liu D.Q., Yang W.X. (2004): Advances in mapping and molecular markers of resistance to leaf rust in wheat. Chinese Agricultural Sciences, 37: 65–71.
 
Long D. L. (1989): A North American System of Nomenclature for Puccinia recondita f. sp. tritici. Phytopathology, 79, 525- https://doi.org/10.1094/Phyto-79-525
 
Moore S.S., Sargeant L.L., King T.J., Mattick J.S., Georges M., Hetzel D.J.S. (1991): The conservation of dinucleotide microsatellites among mammalian genomes allows the use of heterologous PCR primer pairs in closely related species. Genomics, 10, 654-660 https://doi.org/10.1016/0888-7543(91)90448-N
 
Nayar S.K., Prashar M., Kumar J., Bhardwaz S.C., Bhatnagar R. (1991): Pathotypes of Puccinia recondita f.sp. tritici virulent for Lr26 (1BL/1RS translocation) in India. Cereal Research Communications, 19: 327–331.
 
Oelke L. M., Kolmer J. A. (2005): Genetics of Leaf Rust Resistance in Spring Wheat Cultivars Alsen and Norm. Phytopathology, 95, 773-778 https://doi.org/10.1094/PHYTO-95-0773
 
Peterson R. F., Campbell A. B., Hannah A. E. (1948): A DIAGRAMMATIC SCALE FOR ESTIMATING RUST INTENSITY ON LEAVES AND STEMS OF CEREALS. Canadian Journal of Research, 26c, 496-500 https://doi.org/10.1139/cjr48c-033
 
Pretorius Z.A., Roux J.L., Drijepondt S.C. (1990): Occurrence and pathogenicity of Puccinia recondita f.sp. tritici on wheat in South Africa during 1988. Phytophylactica, 22: 225–228.
 
Qiu Ji-Wen, Schürch Anita Christina, Yahiaoui Nabila, Dong Ling-Li, Fan Hua-Jie, Zhang Zhong-Juan, Keller Beat, Ling Hong-Qing (2007): Physical mapping and identification of a candidate for the leaf rust resistance gene Lr1 of wheat. Theoretical and Applied Genetics, 115, 159-168 https://doi.org/10.1007/s00122-007-0551-z
 
Schachermayr G., Siedler H., Gale M.D., Winzeler H., Winzeler M., Keller B. (1994): Identification and localization of molecular markers linked to the Lr9 leaf rust resistance gene of wheat. Theoretical and Applied Genetics, 88, - https://doi.org/10.1007/BF00222402
 
Schachermayr G.M., Messmer M.M., Feuillet C., Winzeler H., Winzeler M., Keller B. (1995): Identification of molecular markers linked to the Agropyron elongatum-derived leaf rust resistance gene Lr24 in wheat. Theoretical and Applied Genetics, 90, - https://doi.org/10.1007/BF00222911
 
Sharp P. J., Kreis M., Shewry P. R., Gale M. D. (1988): Location of ?-amylase sequences in wheat and its relatives. Theoretical and Applied Genetics, 75, 286-290 https://doi.org/10.1007/BF00303966
 
Yan H.F., Yang W.X., Chen Y.F. (2009): Oatgrass E chromosome specific SCAR marker for Lr19 specificity and stability. Journal of Plant Pathology, 39: 42–49.
 
Yuan J.H., Liu T.G., Chen W.Q. (2007): Postulation of leaf rust resistance genes in 47 new wheat cultivars at seedling stage. Scientia Agricultura Sinica, 40: 1925–1935.
 
Zhao X. L., Zheng T. C., Xia X. C., He Z. H., Liu D. Q., Yang W. X., Yin G. H., Li Z. F. (2008): Molecular mapping of leaf rust resistance gene LrZH84 in Chinese wheat line Zhou 8425B. Theoretical and Applied Genetics, 117, 1069-1075 https://doi.org/10.1007/s00122-008-0845-9
 
Zhou Huixin, Xia Xianchun, He Zhonghu, Li Xing, Wang Cuifen, Li Zaifeng, Liu Daqun (2013): Molecular mapping of leaf rust resistance gene LrNJ97 in Chinese wheat line Neijiang 977671. Theoretical and Applied Genetics, 126, 2141-2147 https://doi.org/10.1007/s00122-013-2124-7
 
download PDF

© 2018 Czech Academy of Agricultural Sciences